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中国农学通报

中国农学通报 ›› 2022, Vol. 38 ›› Issue (25): 125-129.doi: 10.11924/j.issn.1000-6850.casb2021-0817

所属专题： 生物技术； 植物保护； 烟草种植与生产

• 植物保护·农药 • 上一篇    下一篇

3株枯草芽孢杆菌对烟草白粉病的防效

蔡永占1(), 王瑞宝1, 白涛1, 刘有才1, 韩小女2, 王斌3(), 华小兵3, 李诚3, 毛明林3, 字文霖3   

  1. 1云南省烟草公司曲靖市公司，云南曲靖 655000
    2宣威市农业技术推广中心，云南宣威 655400
    3微生物菌种筛选与应用国家地方联合工程研究中心，昆明 650217
    4云南省微生物发酵工程研究中心有限公司，昆明 650217
  • 收稿日期:2021-01-22 修回日期:2022-04-02 出版日期:2022-09-05 发布日期:2022-08-26
  • 通讯作者: 王斌
  • 作者简介:蔡永占，男，1984 年出生，河南开封人，农艺师，博士，主要从事烟草病虫害绿色防控。E-mail：caiyongzhan2010@163.com。
  • 基金资助:
    云南省烟草公司科技计划重点项目“曲靖烟草中后期白粉病、赤星病生物防控技术研究与示范”。(2019530000241008)

Control Effect of Three Bacillus subtilis Strains on Tobacco Powdery Mildew

CAI Yongzhan1(), WANG Ruibao1, BAI Tao1, LIU Youcai1, HAN Xiaonv2, WANG Bin3(), HUA Xiaobing3, LI Cheng3, MAO Minglin3, ZI Wenlin3   

  1. 1Qujing Branch of Yunnan Provincial Tobacco Company, Qujing, Yunnan 655000
    2Xuanwei Agricultural Technology Extension Center, Xuanwei, Yunnan 655400
    3National and Local Joint Engineering Research Center for Screening and Application of Microbial Strains, Kunming 650217
    4Yunnan Microbial Fermentation Engineering Research Center Co., Ltd., Kunming 650217
  • Received:2021-01-22 Revised:2022-04-02 Online:2022-09-05 Published:2022-08-26
  • Contact: WANG Bin

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摘要：

为探明3株枯草芽孢杆菌对‘云烟87’白粉病的防效，以枯草芽孢杆菌制得的悬浮剂、菌悬液和低毒化学农药为试验材料，开展其对白粉病防效的研究。室内结果表明，YWF-19-0068、YWF-19-0070、YWF-19-0071生防制剂10倍液对烟草白粉病的防效分别达70.49%、61.15%、69.71%，50倍菌悬液对白粉孢子的萌发抑制率分别达56.92%、56.92%、58.46%。室外结果表明，3株枯草芽孢杆菌制剂不同剂量用药10天后，50倍液的大田防效最优，分别可达46.85%、65.50%、51.70%;选取YWF-19-0070菌株悬浮制剂分别在甲基硫菌灵全量和半量用药一次后，每隔3天使用一次，最后一次用药10天后，甲基硫菌灵全量处理的防效达77.73%以上;甲基硫菌灵半量处理的防效达60.23%以上。

关键词: 枯草芽孢杆菌, 悬浮制剂, 烟草, 白粉病, 防效

Abstract:

To clarify the control effects of three Bacillus subtilis strains on powdery mildew of flue-cured tobacco variety ‘Yunyan 87’, suspensions, bacterial suspension, and suspension agent (SC) prepared by B. subtilis and low-toxic chemical pesticides were used as test materials to carry out research on their control effects on powdery mildew. The indoor results showed that: YWF-19-0068, YWF-19-0070 and YWF-19-0071 biocontrol agents of 10 times dilution had the control effect of 70.49%, 61.15% and 69.71%, respectively. And 50 times dilution of bacterial suspension agents had the germination inhibition rate on the powdery mildew spore of 56.92%, 56.92% and 58.46%, respectively. The outdoor results showed that: 10 days after the application of the three biocontrol agents at different dosages, the control effects of 50 times dilution of bacterial suspension agents were the best of 46.85%, 65.50%, and 51.70%, respectively. After full and half doses of thiophanate were used once, YWF-19-0070 (SC) was applied once every 3 days. 10 days after the last application, the control effect of the full dosage thiophanate methyl treatment was more than 77.73%, and the control effect of the half dosage thiophanate methyl treatment was more than 60.23%.

Key words: Bacillus subtilis, suspension agents, tobacco, powdery mildew, control effect

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