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中国农学通报 ›› 2022, Vol. 38 ›› Issue (25): 120-124.doi: 10.11924/j.issn.1000-6850.casb2021-0919

所属专题: 生物技术 烟草种植与生产

• 植物保护·农药 • 上一篇    下一篇

烟蚜取食对抗、感烟草品种信号分子和CAT活性的影响

孙计平(), 李雪君, 孙焕   

  1. 河南省农业科学院烟草研究所/黄淮烟区烟草病虫害绿色防控重点实验室,河南许昌 461000
  • 收稿日期:2021-09-26 修回日期:2022-06-03 出版日期:2022-09-05 发布日期:2022-08-26
  • 作者简介:孙计平,女,1978 年出生,河北玉田人,助理研究员,博士,研究方向为烟草遗传育种。通信地址:461000 河南省许昌市永昌路与青梅路交叉口烟草所,Tel:0374-3176781,E-mail:sunjiping2002@126.com
  • 基金资助:
    河南省农业科学院自主创新专项基金项目“烟草抗蚜虫种质资源筛选和抗虫机理的研究”(2020ZC17);河南省科技攻关项目“NTH202 基因的抗蚜功能分析和验证”。

Response of Signal Molecules and CAT Activities to Aphid in Resistant and Susceptible Tobacco Varieties

SUN Jiping(), LI Xuejun, SUN Huan   

  1. Tobacco Research Institute, Henan Academy of Agricultural Sciences/ Key Laboratory for Green Preservation & Control of Tobacco Diseases and Pests in Huanghuai Growing Area, Xuchang, Henan 461000
  • Received:2021-09-26 Revised:2022-06-03 Online:2022-09-05 Published:2022-08-26

摘要:

为研究烟草品种抗蚜机制,选用6个不同烟蚜抗性的烟草品种,采用盆栽试验接种蚜虫,研究烟蚜侵袭后第0、3、6、9 h烟草叶片茉莉酸(JA)、一氧化氮(NO)含量以及CAT活性的变化。结果表明,受到蚜虫为害后,叶片JA含量均表现为先增加后降低的趋势,烟蚜取食6 h后叶片JA含量达到峰值,且抗蚜品种JA增量大于感蚜品种。受到蚜虫为害后,抗蚜品种叶片NO含量表现为先增加后减少的趋势,烟蚜取食3 h后NO含量达到峰值,感蚜品种烟蚜取食3 h后的NO含量与取食前差异不显著,但6 h和9 h后显著低于0 h。受到烟蚜侵袭9 h后,参试品种CAT活性均表现为增加趋势,烟蚜取食9 h后,抗蚜品种‘竖把老母鸡2113’的CAT活性增量最多,增幅最大。JA和NO含量以及CAT活性均可以作为烟草品种蚜虫抗性鉴定的生理指标,烟蚜取食5~6 h叶片茉莉酸含量的增幅可以用于鉴定烟草品种抗蚜性。

关键词: 烟蚜, 烟草品种, 防御酶, 信号分子, 抗蚜

Abstract:

To study the mechanism of aphid resistance in tobacco varieties, six tobacco varieties with different aphid resistance were selected and inoculated with aphid in pot experiment. The content change of jasmine acid (JA) and nitric oxide (NO), and CAT activities in tobacco leaves were determined 0, 3, 6 and 9 hours after feeding aphid. The results showed that: after being infested by aphids, the JA content in tobacco leaves increased first, peaked at 6 hours and then decreased. The increment of JA content in resistant varieties was higher than that in susceptible varieties. The NO content in leaves of aphid resistant varieties increased first, peaked at 3 hours and then decreased. The difference of the NO content in leaves of susceptible varieties was not significant between 3 hours’ aphid feeding and 0 h, but decreased significantly after 6 hours and 9 hours. The CAT activity of all tested tobacco varieties showed an increasing trend at 9 h, and CAT activity of aphid resistant variety ‘Shubalaomuji2113’ increased the most. Therefore, JA and NO contents and CAT activity could be used as physiological indicators for aphid resistance identification in tobacco varieties. The increase of JA content in leaves 5-6 hours after inoculation could be used to identify the resistance of tobacco varieties to aphids.

Key words: Myzus persicae, tobacco variety, defensive enzyme, signal molecules, aphid resistance

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