甜菜TIFY基因家族的全基因组鉴定与生物信息学分析

doi:10.11924/j.issn.1000-6850.casb2021-0831

中国农学通报 ›› 2022, Vol. 38 ›› Issue (8): 17-24.doi: 10.11924/j.issn.1000-6850.casb2021-0831

所属专题：生物技术；园艺

甜菜TIFY基因家族的全基因组鉴定与生物信息学分析

巩永永¹^,²^,³(), 端木慧子¹^,²^,³()

¹黑龙江大学农业微生物技术教育部工程研究中心,哈尔滨 150500
²黑龙江大学生命科学学院黑龙江省寒地生态修复与资源利用重点实验室,哈尔滨 150080
³黑龙江大学生命科学学院黑龙江省普通高校分子生物学重点实验室,哈尔滨 150080

收稿日期:2021-08-31 修回日期:2021-11-03 出版日期:2022-03-15 发布日期:2022-04-06
通讯作者: 端木慧子
作者简介:巩永永,男,1998年出生,陕西商洛人,硕士,研究方向：植物分子生态学。通信地址：150080 黑龙江省哈尔滨市学府路74号黑龙江大学生命科学学院320室,E-mail： gongyyong@163.com。
基金资助:
国家自然基金青年项目“甜菜M14品系BvM14-RIN4蛋白盐胁迫响应蛋白互作网络的研究”(31801426);黑龙江省省属高等学校基本科研业务费基础研究项目(科技创新类专项重点项目)“甜菜M14品系与二倍体栽培甜菜的比较转录组学研究”(KJCXZD201717);黑龙江省普通高等学校青年创新人才培养计划“甜菜M14品系BvM14-RIN4基因耐盐功能及其蛋白调控机理研究”(UNPYSCT-2018008)

TIFY Gene Family in Sugar Beet: Whole Genome Identification and Bioinformatics Analysis

GONG Yongyong¹^,²^,³(), DUANMU Huizi¹^,²^,³()

¹Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang University, Harbin 150500
²Heilongjiang Provincial Key Laboratory of Ecological Restoration and Resource Utilization for Cold Region, School of Life Sciences, Heilongjiang University, Harbin 150080
³Key Laboratory of Molecular Biology, College of Heilongjiang Province, Harbin 150080

Received:2021-08-31 Revised:2021-11-03 Online:2022-03-15 Published:2022-04-06
Contact: DUANMU Huizi

摘要/Abstract

摘要：

TIFY转录因子对植物体生长发育和胁迫响应有重要调控作用,本研究目的在于鉴定分析甜菜(Beta vulgaris L.)中的TIFY转录因子。试验以甜菜基因组数据为基础,利用生物信息学技术在全基因组水平鉴定并分析甜菜TIFY家族成员。结果表明：甜菜中共有21条TIFY基因,基因间序列相似性较低;共线性分析发现只有BvTIFY15与BvTIFY18之间存在基因复制事件;BvTIFY转录因子家族包括2个TIFY蛋白、8个JAZ蛋白、6个ZML蛋白、5个PPD蛋白;蛋白互作预测发现,JAZ家族成员对JA信号有重要的调控作用。本研究对BvTIFY基因的结构与功能进行分析,发掘出甜菜根中应答盐胁迫基因BvTIFY13与BvTIFY15,为后续BvTIFY基因的功能研究提供一定理论基础。

关键词: 甜菜, 转录因子, TIFY家族, 全基因组鉴定, 生物信息学

Abstract:

TIFY transcription factors play an important role in regulating plant growth and development as well as stress response, therefore, the purpose of this study is to identify and analyze TIFY transcription factors in sugar beet (Beta vulgaris L.). Based on the genome data of sugar beet, bioinformatics techniques were used to identify and analyze the members of sugar beet TIFY family at the whole genome level. The results showed that there were 21 TIFY genes in sugar beet with low sequence similarity. Collinearity analysis showed that only BvTIFY15 and BvTIFY18 had gene replication events. The family of BvTIFY transcription factors included 2 TIFY proteins, 8 JAZ proteins, 6 ZML proteins and 5 PPD proteins. Protein-protein interaction prediction showed that members of the JAZ family played an important role in the regulation of JA signaling. This study analyzed the structure and function of BvTIFY genes and discovered the salt stress responsive genes BvTIFY13 and BvTIFY15 in sugar beet roots. It can provide a theoretical basis for subsequent functional studies on BvTIFY genes.

Key words: sugar beet, transcription factors, TIFY family, whole genome identification, bioinformatics

中图分类号:

S566.3

巩永永, 端木慧子. 甜菜TIFY基因家族的全基因组鉴定与生物信息学分析[J]. 中国农学通报, 2022, 38(8): 17-24.

GONG Yongyong, DUANMU Huizi. TIFY Gene Family in Sugar Beet: Whole Genome Identification and Bioinformatics Analysis[J]. Chinese Agricultural Science Bulletin, 2022, 38(8): 17-24.

图/表 8

参考文献 13

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基因名	转录本编号	染色体	基因组位置	编码区/bp	蛋白质/aa	分子量/kDa	等电点pI
BvTIFY1	XM_010693544.2	1	177868-181625	1047	348	35.97	9.47
BvTIFY2	XM_010693545.2	1	177868-181625	1047	348	35.97	9.47
BvTIFY3	XM_010673165.2	1	6039574-6047256	1041	346	37.72	5.74
BvTIFY4	XM_019248305.1	1	6039574-6047256	1041	346	37.72	5.74
BvTIFY5	XM_010673216.2	1	6072808-6079282	894	297	32.88	5.94
BvTIFY6	XM_010673225.2	1	6072808-6079282	888	295	32.64	5.94
BvTIFY7	XM_010672448.2	2	39383795-39388815	1119	372	39.43	8.71
BvTIFY8	XM_010695474.2	3	1037984-1064949	1059	352	38.47	8.49
BvTIFY9	XM_010695475.2	3	1037984-1064949	1056	351	38.32	8.5
BvTIFY10	XM_010695476.2	3	1037984-1064949	1050	349	38.14	8.49
BvTIFY11	XM_010695477.2	3	1037984-1064949	1047	348	38.01	8.49
BvTIFY12	XM_010695478.2	3	1037984-1064949	999	332	36.2	8.78
BvTIFY13	XM_010684365.2	6	53385701-53390451	597	198	22.26	8.61
BvTIFY14	XM_010697784.2	7	241127-253491	900	299	32.87	5.94
BvTIFY15	XM_010686977.2	7	36091600-36094215	777	258	29.34	9.24
BvTIFY16	XM_010688927.2	8	19534025-19540778	1050	349	36.08	8.53
BvTIFY17	XM_010688928.2	8	19534025-19540778	1047	348	36.01	8.53
BvTIFY18	XM_010689246.2	8	27921059-27923721	756	251	26.84	9.9
BvTIFY19	XM_010690498.1	9	2254102-2256859	375	124	14.16	9.18
BvTIFY20	XM_010693082.2	9	42035878-42046926	1062	353	38.85	4.97
BvTIFY21	XM_010668415.1	Unknown	29321-30954	372	123	13.71	9.21

基因名	转录本编号	染色体	基因组位置	编码区/bp	蛋白质/aa	分子量/kDa	等电点pI
BvTIFY1	XM_010693544.2	1	177868-181625	1047	348	35.97	9.47
BvTIFY2	XM_010693545.2	1	177868-181625	1047	348	35.97	9.47
BvTIFY3	XM_010673165.2	1	6039574-6047256	1041	346	37.72	5.74
BvTIFY4	XM_019248305.1	1	6039574-6047256	1041	346	37.72	5.74
BvTIFY5	XM_010673216.2	1	6072808-6079282	894	297	32.88	5.94
BvTIFY6	XM_010673225.2	1	6072808-6079282	888	295	32.64	5.94
BvTIFY7	XM_010672448.2	2	39383795-39388815	1119	372	39.43	8.71
BvTIFY8	XM_010695474.2	3	1037984-1064949	1059	352	38.47	8.49
BvTIFY9	XM_010695475.2	3	1037984-1064949	1056	351	38.32	8.5
BvTIFY10	XM_010695476.2	3	1037984-1064949	1050	349	38.14	8.49
BvTIFY11	XM_010695477.2	3	1037984-1064949	1047	348	38.01	8.49
BvTIFY12	XM_010695478.2	3	1037984-1064949	999	332	36.2	8.78
BvTIFY13	XM_010684365.2	6	53385701-53390451	597	198	22.26	8.61
BvTIFY14	XM_010697784.2	7	241127-253491	900	299	32.87	5.94
BvTIFY15	XM_010686977.2	7	36091600-36094215	777	258	29.34	9.24
BvTIFY16	XM_010688927.2	8	19534025-19540778	1050	349	36.08	8.53
BvTIFY17	XM_010688928.2	8	19534025-19540778	1047	348	36.01	8.53
BvTIFY18	XM_010689246.2	8	27921059-27923721	756	251	26.84	9.9
BvTIFY19	XM_010690498.1	9	2254102-2256859	375	124	14.16	9.18
BvTIFY20	XM_010693082.2	9	42035878-42046926	1062	353	38.85	4.97
BvTIFY21	XM_010668415.1	Unknown	29321-30954	372	123	13.71	9.21

甜菜TIFY基因家族的全基因组鉴定与生物信息学分析

TIFY Gene Family in Sugar Beet: Whole Genome Identification and Bioinformatics Analysis

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