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中国农学通报

中国农学通报 ›› 2022, Vol. 38 ›› Issue (32): 22-31.doi: 10.11924/j.issn.1000-6850.casb2021-1119

所属专题： 生物技术； 植物保护

• 林学·园艺·园林 • 上一篇    下一篇

响应辣椒疫霉菌诱导的CaWRKY转录因子筛选及其信号通路分析

徐晓美(), 李颖, 衡周, 徐小万, 李涛, 王恒明()   

  1. 广东省农业科学院蔬菜研究所,蔬菜新技术研究重点实验室,广州 510640
  • 收稿日期:2021-11-23 修回日期:2022-03-05 出版日期:2022-11-15 发布日期:2022-11-09
  • 通讯作者: 王恒明
  • 作者简介:徐晓美,女,1983年出生,江西吉安人,副研究员,博士,研究方向：茄果类蔬菜疫病及分子育种研究。通信地址：530460 广东省广州市天河区五山镇金颖路66号 蔬菜研究所,Tel：020-38469456,E-mail： xiaomeixu@gdaas.cn。
  • 基金资助:
    广东省基础与应用基础研究基金（粤桂联合基金）项目“辣椒根腐疫病候选抗性基因CaRLK功能验证及其与Avr蛋白互作的分子机制研究”(2020A1515410005);广东省基础与应用基础研究基金项目“辣椒根腐疫病候选抗性基因功能验证及其抗病分子机制研究”(2021A1515012132);广东省国际科技合作领域项目“中巴合作蔬菜种质资源创新与新品种推广”(2018A050506052)

CaWRKY Transcription Factors Induced by Phytophthora capsici: Screening and Signal Pathway Analysis

XU Xiaomei(), LI Ying, HENG Zhou, XU Xiaowan, LI Tao, WANG Hengming()   

  1. Key Laboratory for New Technology Research of Vegetables, Vegetable Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640
  • Received:2021-11-23 Revised:2022-03-05 Online:2022-11-15 Published:2022-11-09
  • Contact: WANG Hengming

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摘要：

在辣椒基因组中全面鉴定WRKY转录因子并筛选出受辣椒疫霉菌诱导的CaWRKY基因,并分析关键CaWRKY基因参与的信号通路。以CM334和NMCA10399为材料,基于辣椒基因组和RNA-seq数据,并在水杨酸(SA)和茉莉酸甲酯(MeJA)处理下通过qRT-PCR技术检测基因表达并分析。全基因组共鉴定出69个CaWRKY基因。接菌后12 h,抗、感材料中分别鉴定出7个和3个差异表达基因;接菌后36 h,分别有13个和22个差异表达基因,表明抗病材料能更快速应答。在筛选出的8个关键CaWRKY基因中,CaWRKY19和CaWRKY65受SA诱导上调表达;CaWRKY50受MeJA诱导上调表达;CaWRKY25受SA诱导上调表达同时受到MeJA抑制下调表达,而CaWRKY49同时受SA和MeJA抑制下调表达,推测CaWRKY19和CaWRKY65通过SA,CaWRKY50通过JA,而CaWRKY25和CaWRKY49则通过SA和JA信号途径参与辣椒抗疫病防御反应。

关键词: 辣椒, 疫病, WRKY转录因子, 诱导, 信号通路

Abstract:

The aims of this study are to identify WRKY transcription factors in the whole genome of pepper, select the CaWRKYs induced by Phytophthora capsici and analyze the signal pathways involved in the key CaWRKY genes. CM334 and NMCA10399 were used as experimental materials. Based on genome and RNA-seq data, gene expressions were obtained by qRT-PCR under the treatment of salicylic acid (SA) and methyl jasmonate (MeJA). The results showed that 69 CaWRKYs were identified in the whole genome of pepper. At the time point of 12 hpi, there were 7 and 3 CaWRKYs significantly and differentially expressed in resistant and susceptible materials, respectively. At 36 hpi, 13 and 22 genes were significantly and differentially expressed in resistant and susceptible materials, respectively. These results indicated that resistant material could respond more quickly to the pathogens. Among the 8 key CaWRKYs selected, CaWRKY19 and CaWRKY65 were induced by SA and up-regulated expressed, while CaWRKY50 was induced by MeJA and up-regulated expressed. CaWRKY25 was up-regulated by SA and down-regulated by MeJA, while CaWRKY49 was down-regulated by both SA and MeJA. Signal pathway analysis speculated that CaWRKY19 and CaWRKY65 were involved in pepper defense against Phytophthora through SA signal pathway, CaWRKY50 through JA signal pathway, while CaWRKY25 and CaWRKY49 through both SA and JA signal pathway.

Key words: pepper, Phytophthora blight, WRKY transcription factor, induce, signal pathway

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