关键词: 西番莲, 细胞悬浮系, 愈伤组织, 诱导, 生长曲线

Abstract:

The aim of this study is to establish a rapid and stable cell suspension culture system for passion fruit callus, and to optimize the culture conditions, which may lay a foundation for genetic engineering of passion fruit. The seedlings of passion fruit were used as plant materials and their different parts were used as explants to induce callus, which were then used to develop cell suspension culture. Single factor and orthogonal experiment were carried out to optimize the cell suspension culture conditions. Results showed that the basic medium MS with 2 mg/L 2,4-D was suitable for the induction of callus of passion fruit while the seedling hypocotyl was suitable as explants,with a callus induction rate of 83.3%. Under the optimal culture condition of MS-based medium supplemented with 2 mg/L 2,4-D, 0.2 g/L Glutamine and 30 g/L sucrose, and pH of 6.1, a rapid and stable suspension culture system for passion fruit was established. The initial cell dosage was 6.0 mg/mL and shaker speed was 100 r/min, and 28℃ dark culture was adopted. Under the above growth condition, the suspension cells grew uniformly and vigorously. The growth curve of the suspension cells was S-shaped,and the logarithmic phase was on the 12-18 d after inoculation and the cells were cultured under suitable conditions. This study lays a material and technology base for cell engineering breeding and genetic transformation system of passion fruit, which is important for the development of passion fruit industry.

Key words: passion fruit, cell suspension culture, callus, induction, growth curve