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中国农学通报

中国农学通报 ›› 2022, Vol. 38 ›› Issue (19): 18-24.doi: 10.11924/j.issn.1000-6850.casb2021-0231

所属专题： 生物技术； 园艺

• 林学·园艺·园林 • 上一篇    下一篇

桃叶卫矛茎段愈伤组织诱导及不定芽再生体系的建立

李瑞静1(), 田菊1,2, 刘洋1,2()   

  1. 1内蒙古和盛生态科技研究院有限公司,呼和浩特 011517
    2内蒙古农业大学,呼和浩特 010019
  • 收稿日期:2021-03-09 修回日期:2022-05-26 出版日期:2022-07-05 发布日期:2022-07-13
  • 通讯作者: 刘洋
  • 作者简介:李瑞静,女,1993年出生,内蒙古杭锦旗人,技术员,硕士,研究方向为林木新品种繁殖技术研究。通信地址：011517 内蒙古呼和浩特市盛乐经济园区,E-mail： LRJ940124@163.com。
  • 基金资助:
    呼和浩特市重大科技专项“呼市生态脆弱区和生态保护区修复与保护的关键技术研究与示范”(2019-社-重-1)

The Callus Induction for Stem Segment and Adventitious Bud Regeneration System of Euonymus maackii

LI Ruijing1(), TIAN Ju1,2, LIU Yang1,2()   

  1. 1Inner Mongolia Hesheng Ecological Science and Technology Research Institute Company Limited, Hohhot 011517
    2Inner Mongolia Agricultural University, Hohhot 010019
  • Received:2021-03-09 Revised:2022-05-26 Online:2022-07-05 Published:2022-07-13
  • Contact: LIU Yang

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摘要：

为完善及优化桃叶卫矛组培繁殖再生体系,解决以腋芽萌发为再生途径进行组培增殖时增殖系数小的问题,以桃叶卫矛幼嫩茎段为材料,分别用20%的次氯酸钠和0.10%的升汞进行消毒处理,处理时间分别为8、10、12 min获取其消毒最佳方案;以桃叶卫矛茎段为材料,通过调节6-BA和NAA浓度,诱导桃叶卫矛茎段产生愈伤组织;以桃叶卫矛愈伤组织为材料,采用6-BA和NAA正交试验诱导产生不定芽;以桃叶卫矛不定芽为材料,调节6-BA和NAA浓度,提高继代增殖系数;以桃叶卫矛继代增殖苗为材料,调节IBA和NAA浓度,获取桃叶卫矛生根苗。研究结果表明：（1）桃叶卫矛幼嫩茎段消毒最佳方案为20%的次氯酸钠处理12 min,污染率2%;（2）茎段愈伤组织诱导最佳培养基为1.0 mg/L 6-BA+ 0.10 mg/L NAA,诱导率80.00%;（3）不定芽诱导最佳培养基为0.5 mg/L 6-BA+ 0.10 mg/L NAA,诱导率91.11%;（4）继代增殖最佳培养基为MS+ 1.0 mg/L 6-BA+ 0.10 mg/L NAA,增殖系数6;（5）生根培养最佳培养基为1/2MS+ IBA 0.3 mg/L,生根条数平均为5条。本研究利用桃叶卫矛无芽茎段诱导出愈伤组织,并将继代增殖系数提高至6,且完成了桃叶卫矛组培再生体系的优化。

关键词: 桃叶卫矛, 愈伤组织, 不定芽, 再生体系, 继代增殖

Abstract:

The study aims to optimize the tissue culture reproduction and regeneration system of Euonymus maackii, solve the problem of small multiplication coefficient when the axillary bud germination is used as the regeneration method for tissue culture. Taking the young stems of E. maackii as the materials, we sterilized them with 20% sodium hypochlorite and 0.1% mercuric chloride respectively, and set the treatment time as 8, 10 and 12 minutes, to obtain the best sterilization plan. Using E. maackii stems as the materials, by adjusting the concentration of 6-BA and NAA, the stems of E. maackii were induced to produce callus. The callus of E. maackii was used as the material, and the adventitious buds were induced by 6-BA and NAA orthogonal test. Using the adventitious buds of E. maackii as the materials, we adjusted the concentration of 6-BA and NAA to increase the proliferation coefficient of subsequent generations. Using E. maackii seedlings as the materials, we adjusted the concentration of IBA and NAA to obtain rooting seedlings of E. maackii. The results showed that: (1) the best solution for sterilizing the young stems of E. maackii was 20% sodium hypochlorite for 12 minutes, and the pollution rate was 2%; (2) the best medium for stem callus induction was 1.0 mg/L 6-BA+ 0.10 mg/L NAA, and the induction rate was 80.00%; (3) the best medium for adventitious bud induction was 0.5 mg/L 6-BA+ 0.10 mg/L NAA, and the induction rate was 91.11%; (4) the best medium for subsequent proliferation was MS+1.0 mg/L 6-BA+ 0.10 mg/L NAA, and the proliferation coefficient was 6; (5) the best medium for rooting culture was 1/2MS+IBA 0.3 mg/L, and the average number of roots was 5. The callus is induced from the budless stems of E. maackii, and the proliferation coefficient is increased to 6. The optimization of the tissue culture regeneration system of E. maackii is achieved.

Key words: Euonymus maackii, callus induction, adventitious bud, regeneration system, subsequent proliferation

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