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中国农学通报

中国农学通报 ›› 2018, Vol. 34 ›› Issue (28): 78-81.doi: 10.11924/j.issn.1000-6850.casb17070130

所属专题： 生物技术

• 林学 园艺 园林 • 上一篇    下一篇

钩藤愈伤组织的诱导与植株再生

吴顺,田维雅,周凯,胡俊宏,伍信红,刘坤   

  1. 中南林业科技大学生命科学与技术学院,中南林业科技大学生命科学与技术学院,中南林业科技大学生命科学与技术学院,中南林业科技大学生命科学与技术学院,中南林业科技大学生命科学与技术学院,中南林业科技大学生命科学与技术学院
  • 收稿日期:2017-07-26 修回日期:2018-09-06 接受日期:2017-12-15 出版日期:2018-10-11 发布日期:2018-10-11
  • 通讯作者: 吴顺
  • 基金资助:
    湖南省教育厅科学研究项目“钩藤毛状根的诱导及其代谢调控研究”(16C1677)；中南林业科技大学大学生研究性计划与创新性项目“钩藤 毛状根的诱导与植株再生”[(Z)2017-00983-10]。

Uncaria rhynchophylla (Mip.) Jack: Callus Induction and Plantlet Regeneration

  • Received:2017-07-26 Revised:2018-09-06 Accepted:2017-12-15 Online:2018-10-11 Published:2018-10-11

摘要： 为了建立钩藤愈伤组织诱导与植株再生体系。以药用植物钩藤[Uncaria rhynchophyllai (Mip.)Jack]嫩茎、嫩枝、嫩叶为外植体，通过优化激素组合，进行愈伤组织诱导和分化、幼苗生根和移栽，初步建立了钩藤愈伤组织诱导和植株再生体系。结果表明，以钩藤嫩枝作为外植体出愈情况较好，污染率低，继代后愈伤组织生长快。培养基成分为WPM+2.0 mg/L 2,4-D+0.5 mg/L 6-BA较适宜愈伤组织的诱导和增殖，诱导率可达83.3%。培养基成分为WPM+2.0 mg/L KT+0.2 mg/L NAA能分化出芽，愈伤组织率分化率达到82.3%；而1/2WPM+1.0 mg/L IBA适宜于试管苗生根，生根率达92.0%。以钩藤嫩枝为外植体诱导获得了质量良好的愈伤组织，并成功再生成植株，结果的获得可为解决钩藤人工栽培过程的资源和品质问题以及进行细胞培养和分子遗传改良奠定基础。

Abstract: The paper aims to build callus induction and plantlet regeneration system for Uncaria rhynchophylla (Mip.) Jack. The authors took the fresh stems, shoots and leaves as explants to conduct the induction and differentiation of callus, and the rooting and transplantation of seedlings by optimizing hormone combination, to establish the callus induction and plantlet regeneration system of Uncaria rhynchophylla (Mip.) Jack. The results showed that: the callus of the fresh shoot of Uncaria rhynchophylla (Mip.) Jack as explant was better, its pollution rate was low, and the callus growth was faster after subculture; the optimal medium for callus induction was WPM+2.0 mg/L 2,4-D+0.5 mg/L 6-BA; for re-differentiation WPM+2.0 mg/L KT+0.2 mg/L NAA; and for rooting 1/2WPM+1.0 mg/L IBA; the callus induction, re-differentiation and rooting rate was 83.3%, 82.3% and 92%, respectively. In conclusion, the callus is induced by the explants of fresh shoots and the plants are regenerated successfully, and the results obtained can be used to solve the resource and quality problems of the artificial cultivation of Uncaria rhynchophylla (Mip.) Jack, and to lay a foundation for cell culture and molecular genetic improvement.