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中国农学通报 ›› 2018, Vol. 34 ›› Issue (28): 73-77.doi: 10.11924/j.issn.1000-6850.casb18050162

• 林学 园艺 园林 • 上一篇    下一篇

粗糙龙胆再生根发育形态学研究

张健男,孟庆艳,张欣欣,孙阎   

  1. 黑龙江大学 农业资源与环境学院,黑龙江大学农业资源与环境学院,哈尔滨师范大学 生命科学与技术学院,黑龙江大学 农业资源与环境学院
  • 收稿日期:2018-05-31 修回日期:2018-07-13 接受日期:2018-07-24 出版日期:2018-10-11 发布日期:2018-10-11
  • 通讯作者: 孙阎
  • 基金资助:
    黑龙江省自然科学基金项目“龙胆属不同生态类型植物个体发育早期研究”(C2015024);黑龙江省大学生创新创业训练计划项目“粗糙龙 胆地下器官再植技术研究”(201710212043)。

Developmental Morphology of Regenerative Roots of Gentiana scabra

  • Received:2018-05-31 Revised:2018-07-13 Accepted:2018-07-24 Online:2018-10-11 Published:2018-10-11

摘要: 为了解粗糙龙胆断根截面再生根的发育过程,本研究采用常规石蜡切片技术,对粗糙龙胆断根取药后再植发育过程进行了研究。结果表明:3年生粗糙龙胆不定根结构由皮层和维管柱组成,皮层外侧未见周皮,形成层也未构成完整环形,次生木质部不发达。断根面不定根是由形成层处的一些细胞分裂而形成的,这些细胞快速分裂形成分生细胞团,分生细胞团经单向极性生长,直接形成根原基,断根面可发育形成多个根原基;不定根发生时未见愈伤组织形成,为直接器官发生类型。不同植株再生根发生时间略有不同,同一根系内也有发育不同步现象。在室内条件下,再生根发生周期约为15天,这些再生根至当年秋季即可长到一定的长度和粗度。因此,粗糙龙胆断根面再生根的发生属直接器官发生类型,分生细胞团起源于形成层附近的一些细胞,这些再生根经过一个生长季的发育即可符合采收标准。

关键词: 大丽花、品种资源、产业链、发展现状、对策, 大丽花、品种资源、产业链、发展现状、对策

Abstract: To understand the development process of regenerative roots on the excised roots, this study used conventional paraffin sectioning techniques to explore the replantation development process on the excised root after taking medicinal ingredients in Gentiana scabra. The results showed that the adventitious root structure of three-year-old G. scabra was composed of cortex and vascular cylinder. There was no periderm on the lateral cortex, and the formation layer did not form a complete ring. The secondary xylem was not developed. The adventitious roots on the excised root were formed by some cambium cells division, and then these cells divided rapidly to form the meristematic cell clusters. These meristematic cell clusters grew in a unidirectional polarity and formed root primordia directly. Multiple root primordia were formed from the excised root surface. The occurrence of adventitious roots, without the formation of callus, belonged to the type of direct organogenesis. The regeneration time of different plants was slightly different, and there was also developmental unsynchronization in the same root system. Under indoor condition, the regeneration cycle of reproductive roots took about 15 days. These roots could grow to a certain length and thickness in the autumn of that year. Therefore, the regenerative roots of rough gentian roots belong to the type of direct organogenesis. The meristematic cell masses originate from some cells near the formation layer. These regenerative roots can meet the harvesting standards after a growing season.