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中国农学通报 ›› 2020, Vol. 36 ›› Issue (18): 39-43.doi: 10.11924/j.issn.1000-6850.casb19030018

所属专题: 生物技术 园艺

• 林学·园艺·园林 • 上一篇    下一篇

水杨酸诱导对苹果体内防御酶活性的影响

侯珲1,2, 周增强2(), 王丽2, 王生荣1()   

  1. 1甘肃农业大学植物保护学院,兰州 730000
    2中国农业科学院郑州果树研究所,郑州 450009
  • 收稿日期:2019-03-05 修回日期:2019-04-28 出版日期:2020-06-25 发布日期:2020-07-10
  • 通讯作者: 周增强,王生荣
  • 作者简介:侯珲,女,1977年出生,河南郑州人,副研究员,博士,主要从事果树病害研究。通信地址:450009 郑州市航海东路63中南郑州果树研究所,Tel:0371-65330953,E-mail: houhui@caas.cn。
  • 基金资助:
    中国农业科学院科技创新工程项目“果树病虫害防控”(CAAS-ASTIP);河南省基础与前沿技术研究计划“利用RNA-seq技术探索苹果轮纹病菌致病相关基因的功能”(152300410233);中国农业科学院基本科研业务费专项“苹果轮纹病绿色防控技术研究与示范”(1610192019506);河南省科技攻关项目“苹果生产中杀菌剂使用过程控制技术研究”(182102110451)

Effects Induced by Alicylic Acid on Defense Enzyme Activities in Apple

Hou Hui1,2, Zhou Zengqiang2(), Wang Li2, Wang Shengrong1()   

  1. 1Gansu Agricultural University, College of Plant Protection, Lanzhou 730000
    2Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009
  • Received:2019-03-05 Revised:2019-04-28 Online:2020-06-25 Published:2020-07-10
  • Contact: Zhou Zengqiang,Wang Shengrong

摘要:

为研究叶面喷施水杨酸(SA)对不同苹果品种防御酶活性的影响,以抗病品种‘北之幸’和感病品种‘礼泉短富’为试验材料,对不同浓度水杨酸处理前后的苹果叶片进行防御酶活性测定。结果表明,经水杨酸处理后,‘北之幸’和‘礼泉短富’的β-1,3-葡聚糖酶、POD、PAL和PPO活性均有提高,且高于对照植株。其中‘北之幸’的β-1,3-葡聚糖酶、POD、PAL和PPO活性分别是对照的2.04倍,6.19倍,2.73倍和2.32倍;‘礼泉短富’的β-1,3-葡聚糖酶和PAL活性分别是对照的1.82倍和1.70倍,POD活性是对照的5.68倍,PPO活性是对照的4.00倍。由此可见,水杨酸处理可以不同程度的提高植株体内抗性相关酶的活性,但不同品种间存在差异。

关键词: 苹果, 水杨酸, 酶活性, 诱导抗性

Abstract:

To study the activities of defense enzymes in apple by leaf-spraying salicylic acid (SA), the resistant variety ‘Kitanosach’ and the susceptible variety ‘Liquanduanfu’ were used as materials, the activities of defense enzymes in the leaves were detected before and after different-concentration SA treatments. The results showed that after treated by salicylic acid, the β-1,3-glucosidase, POD, PAL and PPO activities of ‘Kitanosach’ and ‘Liquanduanfu’ increased and were higher than those of the control. The activity of β-1,3-glucanase, POD, PAL and PPO in ‘Kitanosach’ was 2.04 times, 6.19 times, 2.73 times and 2.32 times of those of the control, respectively. The activity of β-1,3-glucanase, PAL POD and PPO in ‘Liquanduanfu’ was 1.82 times, 1.70 times, 5.68 times and 4.00 times of those of the control, respectively. Therefore, the activities of defense enzymes can be increased by different degrees after treated by salicylic acid, but the resistance of different varieties was different.

Key words: apple, salicylic acid, enzyme activity, induced resistance

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