中国农学通报 ›› 2022, Vol. 38 ›› Issue (28): 136-142.doi: 10.11924/j.issn.1000-6850.casb2022-0201
唐玲1,2(), 孙思琦1,2, 闫桦1,2, 刘亚昕1,2, 刘大丽1,2()
收稿日期:
2022-03-24
修回日期:
2022-06-15
出版日期:
2022-10-05
发布日期:
2022-09-28
通讯作者:
刘大丽
作者简介:
唐玲,女,1997年出生,四川巴中人,硕士在读,研究方向:作物。通信地址:150080 黑龙江省哈尔滨市南岗区学府路74号 黑龙江大学现代农业与生态环境学院,Tel:0451-86609494,E-mail: 基金资助:
TANG Ling1,2(), SUN Siqi1,2, YAN Hua1,2, LIU Yaxin1,2, LIU Dali1,2()
Received:
2022-03-24
Revised:
2022-06-15
Online:
2022-10-05
Published:
2022-09-28
Contact:
LIU Dali
摘要:
甜菜红素是一种无毒无害的天然色素,因高水溶性、高效着色性及多种与健康相关的生物学特性而广受欢迎。但是甜菜红素的提取率极不稳定,且提取出来的色素纯度低。为了进一步明晰甜菜红素的提取及纯化技术,笔者总结了近几年国内外学者对甜菜红素提取及纯化技术的研究探索。发现传统提取技术如溶剂提取法、连续扩散法,提取效率低、色素损失大且污染环境。新兴技术如超声波、微波等辅助技术,结合大孔树脂吸附、膜分离等纯化手段,克服了传统提取技术的缺点,将是未来的重点发展方向。另外,笔者从食品、药品与保健品、工业等角度对甜菜红素的应用进行了归纳,并期待甜菜红素未来可以在医药、提取与纯化工艺以及红素来源等方面有所突破。
中图分类号:
唐玲, 孙思琦, 闫桦, 刘亚昕, 刘大丽. 甜菜红素提取与纯化技术的研究进展[J]. 中国农学通报, 2022, 38(28): 136-142.
TANG Ling, SUN Siqi, YAN Hua, LIU Yaxin, LIU Dali. A Review of Betacyanin Extraction and Purification[J]. Chinese Agricultural Science Bulletin, 2022, 38(28): 136-142.
[16] |
THAKUR V, GUPTA D K D. Studies on the clarification and concentration of beetroot juice[J]. Journal of food processing and preservation, 2006,30(2):194-207.
doi: 10.1111/j.1745-4549.2006.00059.x URL |
[17] | TIWARI B K, CULLEN P J. Extraction of red beet pigments[M]. Red beet biotechnology, Springer, Boston, MA, 2013:373-391. |
[18] |
MARAN J P, PRIYA B. Multivariate statistical analysis and optimization of ultrasound-assisted extraction of natural pigments from waste red beet stalks[J]. Journal of food science and technology, 2016,53:792-799.
doi: 10.1007/s13197-015-1988-8 pmid: 26788000 |
[19] |
TOMA M, VINATORU M, PANIWNYK L, et al. Investigation of the effects of ultrasound on vegetal tissues during solvent extraction[J]. Ultrasonics sonochemistry, 2001,8(2):137-142.
pmid: 11326609 |
[20] |
AZMIR J, ZAIDUL I S M, RAHMAN M M, et al. Techniques for extraction of bioactive compounds from plant materials: A review[J]. Journal of food engineering, 2013,117(4):426-436.
doi: 10.1016/j.jfoodeng.2013.01.014 URL |
[21] | FERNANDO G S N, WOOD K, PAPAIOANNOU E H, et al. Application of an ultrasound-assisted extraction method to recover betalains and polyphenols from red beetroot waste[J]. ACS sustainable chemistry & engineering, 2021,9(26):8736-8747. |
[22] | BESZéDES S, PONGRáCZ E, HODúR C. Microwave-assisted extraction of anthocyanins from black currant marc[J]. Food and bioprocess technology, 2012. |
[23] |
THIRUGNANASAMBANDHAM K, SIVAKUMAR V. Microwave assisted extraction process of betalain from dragon fruit and its antioxidant activities[J]. Journal of the Saudi society of agricultural sciences, 2017,16(1):41-48.
doi: 10.1016/j.jssas.2015.02.001 URL |
[24] |
CARDOSO-UGARTE G A, SOSA-MORALES M E, BALLARD T, et al. Microwave-assisted extraction of betalains from red beet (Beta vulgaris)[J]. LWT-food science and technology, 2014,59(1):276-282.
doi: 10.1016/j.lwt.2014.05.025 URL |
[25] | KOUBAA M, BARBA F J, GRIMI N, et al. Recovery of colorants from red prickly pear peels and pulps enhanced by pulsed electric field and ultrasound[J]. Innovative food science & emerging technologies, 2016,37:336-344. |
[26] |
LOGINOVA K V, LEBOVKA N I, VOROBIEV E. Pulsed electric field assisted aqueous extraction of colorants from red beet[J]. Journal of food engineering, 2011,106(2):127-133.
doi: 10.1016/j.jfoodeng.2011.04.019 URL |
[27] |
ZIMMERMANN U, PILWAT G, BECKERS F, et al. Effects of external electrical fields on cell membranes[J]. Bioelectrochemistry and bioenergetics, 1976,3(1):58-83.
doi: 10.1016/0302-4598(76)85007-6 URL |
[28] |
LUENGO E, MARTíNEZ J M, ÁLVAREZ I, et al. Effects of millisecond and microsecond pulsed electric fields on red beet cell disintegration and extraction of betanines[J]. Industrial crops and products, 2016,84:28-33.
doi: 10.1016/j.indcrop.2016.01.016 URL |
[29] | VISOCKIS M, BOBINAITė R, RUZGYS P, et al. Assessment of plant tissue disintegration degree and its related implications in the pulsed electric field (PEF)-assisted aqueous extraction of betalains from the fresh red beetroot[J]. Innovative food science & emerging technologies, 2021,73:102761. |
[30] |
FU Y, SHI J, XIE S Y, et al. Red beetroot betalains: Perspectives on extraction, processing, and potential health benefits[J]. Journal of agricultural and food chemistry, 2020,68(42):11595-11611.
doi: 10.1021/acs.jafc.0c04241 pmid: 33040529 |
[31] |
NAYAK C A, CHETHANA S, RASTOGI N K, et al. Enhanced mass transfer during solid-liquid extraction of gamma-irradiated red beetroot[J]. Radiation physics and chemistry, 2006,75(1):173-178.
doi: 10.1016/j.radphyschem.2005.03.015 URL |
[32] |
LATORRE M E, NARVAIZ P, ROjAS A M, et al. Effects of gamma irradiation on bio-chemical and physico-chemical parameters of fresh-cut red beet (Beta vulgaris L. var. conditiva) root[J]. Journal of food engineering, 2010,98(2):178-191.
doi: 10.1016/j.jfoodeng.2009.12.024 URL |
[33] | 于志云, 尹健. 超临界二氧化碳萃取技术在辣椒红色素精制工艺中的应用[J]. 中国食品添加剂, 2001(5):63-66. |
[34] | IDHAM Z, PUTRA N R, AZIZ A H A, et al. Improvement of extraction and stability of anthocyanins, the natural red pigment from roselle calyces using supercritical carbon dioxide extraction[J]. Journal of CO2 utilization, 2022,56:101839. |
[35] | 王成. 甜菜红色素的提取工艺及其理化性质研究[D]. 南京: 南京工业大学, 2003. |
[1] |
KHAN M I. Stabilization of betalains: a review[J]. Food chemistry, 2016,197(Part B):1280-1285.
doi: 10.1016/j.foodchem.2015.11.043 URL |
[2] | NEELWARNE B, HALAGUR S B. Red beet: An overview[M]. Red beet biotechnology, 2013:1-43. |
[36] |
ZIN M M, MáRKI E, BáNVöLGYI S. Evaluation of reverse osmosis membranes in concentration of beetroot peel extract[J]. Periodica polytechnica chemical engineering, 2020,64(3):340-348.
doi: 10.3311/PPch.15040 URL |
[37] | 王金亭, 鞠秀萍, 吴广庆. 大孔吸附树脂纯化紫荆花红色素的研究[J]. 食品研究与开发, 2009,30(4):49-53. |
[3] |
SVENSON J, SMALLFIELD B M, JOYCE N I, et al. Betalains in red and yellow varieties of the andean tuber crop ulluco (Ullucus tuberosus)[J]. Journal of agricultural and food chemistry, 2008,56(17):7730-7737.4
doi: 10.1021/jf8012053 pmid: 18662012 |
[4] |
SCHWARTZ S J, VON ELBE J H. Quantitative determination of individual betacyanin pigments by high-performance liquid chromatography[J]. Journal of agricultural and food chemistry, 1980,28(3):540-543.
doi: 10.1021/jf60229a032 URL |
[38] | 陈宇, 林旺, 许琳. 大孔树脂分离纯化火龙果红色素的工艺研究[J]. 韶关学院学报, 2017,38(9):56-60. |
[39] | 楚朝霞. 红甜菜中甜菜红素的提取纯化及其微胶囊制备的研究[D]. 哈尔滨: 哈尔滨工业大学, 2021. |
[5] | 吕晓玲, 王玉平, 周平. 等. 甜菜红色素主要成分抗氧化能力[J]. 食品研究与开发, 2009,30(6):39-43. |
[6] |
RAVICHANDRAN K, PALANIRAj R, SAW N M M T, et al. Effects of different encapsulation agents and drying process on stability of betalains extract[J]. Journal of food science and technology, 2014,51(9):2216-2221.
doi: 10.1007/s13197-012-0728-6 pmid: 25190886 |
[40] | 吕思润. 甜菜红素的提取纯化及其生物活性研究[D]. 哈尔滨: 哈尔滨工业大学, 2016. |
[41] | 段迪. 盐地碱蓬甜菜红素提取分离及纯化工艺的研究[D]. 济南: 山东师范大学, 2009. |
[7] |
GANDíA-HERRERO F, GARCíA-CARMONA F. Biosynthesis of betalains: yellow and violet plant pigments[J]. Trends in plant science, 2013,18(6):334-343.
doi: 10.1016/j.tplants.2013.01.003 URL |
[8] |
GEORGIEV V G, WEBER J, KNESCHKE E M, et al. Antioxidant activity and phenolic content of betalain extracts from intact plants and hairy root cultures of the red beetroot Beta vulgaris cv. Detroit dark red[J]. Plant foods for human nutrition, 2010,65(2):105-111.
doi: 10.1007/s11130-010-0156-6 URL |
[42] |
WALKOWIAK-TOMCZAK D. Microbiological denitrification of red beet juice[J]. European food research and technology, 2002,215(5):401-406.
doi: 10.1007/s00217-002-0583-1 URL |
[43] |
GENGATHARAN A, DYKES G A, CHOO W S. Fermentation of red pitahaya extracts using Lactobacillus spp. and Saccharomyces cerevisiae for reduction of sugar content and concentration of betacyanin content[J]. Journal of food science and technology, 2021,58(9):3611-3621.
doi: 10.1007/s13197-021-05116-2 URL |
[9] |
TANAKA Y, SASAKI N, OHMIYA A. Biosynthesis of plant pigments: anthocyanins, betalains and carotenoids[J]. The plant journal, 2008,54(4):733-749.
doi: 10.1111/j.1365-313X.2008.03447.x pmid: 18476875 |
[10] | PAVOKOVIć D, KRSNIK-RASOL M. Complex biochemistry and biotechnological production of betalains[J]. Food technology and biotechnology, 2011,49(2):145-155. |
[44] |
MEREDDY R, CHAN A, FANNING K, et al. Betalain rich functional extract with reduced salts and nitrate content from red beetroot (Beta vulgaris L.) using membrane separation technology[J]. Food chemistry, 2017,215:311-317.
doi: 10.1016/j.foodchem.2016.07.132 URL |
[45] | 周宏璐, 王景会. 甜菜红色素提取、精制、稳定化研究[J]. 吉林农业科学, 2010,35(6):58-62. |
[11] | 刘冰. 火龙果果皮甜菜红素提取及抗氧化保护作用研究[D]. 锦州: 锦州医科大学, 2018. |
[12] | GUINé R, MENDES M, GONçALVES F. Optimization of bioactive compound’s extraction conditions from beetroot by means of artificial neural networks (ANN)[J]. Agricultural engineering international: CIGR Journal, 2019,21(4):216-223. |
[46] | 李崇瑛, 王安, 杨涛, et al. 食用天然色素的纯化与研究进展[J]. 中国调味品, 2007(9):18-22,38. |
[47] |
KANNER J, HAREL S, GRANIT R. Betalains a new class of dietary cationized antioxidants[J]. Journal of agricultural and food chemistry, 2001,49(11):5178-5185.
doi: 10.1021/jf010456f URL |
[13] | STURZOIU A, STROESCU M, STOICA A, et al. Betanine extraction from Beta vulgaris-experimental research and statistical modeling[J]. UPB scientific bulletin, series B: chemistry and materials science, 2011,73(1):145-56. |
[14] | 熊勇, 张水军, 李冬梅. 甜菜红色素提取工艺及其稳定性研究[J]. 中国食品添加剂, 2015(6):79-85. |
[48] |
SCHLIEMANN W, JOY IV R W, KOMAMINE A, et al. Betacyanins from plants and cell cultures of Phytolacca americana[J]. Phytochemistry, 1996,42(4):1039-1046.
doi: 10.1016/0031-9422(96)00100-8 URL |
[49] |
CHETHANA S, NAYAK C A, RAGHAVARAO K. Aqueous two phase extraction for purification and concentration of betalains[J]. Journal of food engineering, 2007,81(4):679-687.
doi: 10.1016/j.jfoodeng.2006.12.021 URL |
[15] | 晏兴珠, 毛琪, 王仕玉. 等. 藜麦甜菜红素的提取优化及稳定性研究[J]. 食品与发酵科技, 2021,57(3):44-48. |
[50] | AHMADI H, NAYERI Z, MINUCHEHR Z, et al. Betanin purification from red beetroots and evaluation of its anti-oxidant and anti-inflammatory activity on LPS-activated microglial cells[J]. Plos one, 2020,15(5):e023088. |
[51] |
GONçALVES L C P, DE SOUZA TRASSI M A, LOPES N B, et al. A comparative study of the purification of betanin[J]. Food chemistry, 2012,131(1):231-238.
doi: 10.1016/j.foodchem.2011.08.067 URL |
[52] | ESATBEYOGLU T, WAGNER A E, SCHINI-KERTH V B, et al. Betanin-A food colorant with biological activity[J]. Molecular nutrition & food research, 2015,59(1):36-47. |
[53] |
DELGADO-VARGAS F, JIMéNEZ A R, PAREDES-LóPEZ O. Natural pigments: carotenoids, anthocyanins, and betalains-characteristics, biosynthesis, processing, and stability[J]. Critical reviews in food science and nutrition, 2000,40(3):173-289.
doi: 10.1080/10408690091189257 URL |
[54] |
FALOWO A B, FAYEMI P O, MUCHENjE V. Natural antioxidants against lipid-protein oxidative deterioration in meat and meat products: A review[J]. Food research international, 2014,64:171-181.
doi: S0963-9969(14)00417-7 pmid: 30011637 |
[55] |
DOWNHAM A, COLLINS P. Colouring our foods in the last and next millennium[J]. International journal of food science and technology, 2000,35(1):5-22.
doi: 10.1046/j.1365-2621.2000.00373.x URL |
[56] |
KHAN M I. Plant betalains: Safety, antioxidant activity, clinical efficacy, and bioavailability[J]. Comprehensive reviews in food science and food safety, 2016,15(2):316-330.
doi: 10.1111/1541-4337.12185 URL |
[57] | 张忠平, 杨旭. 红甜菜果醋生产工艺的研究[J]. 中国调味品, 2018,43(8):101-103. |
[58] | 陈克宁, 付聪颖, 李建东. 甜菜红用于羊毛染色的研究[J]. 染料与染色, 2006(1):21-24. |
[59] | 张艳, 张伟, 刘德驹. 甜菜红色素对蚕丝织物染色工艺研究[J]. 丝绸, 2011,48(6):4-6,16. |
[60] |
PATNI N G, PILLAI S, SHARMA P. Effect of using betalain, anthocyanin and chlorophyll dyes together as a sensitizer on enhancing the efficiency of dye-sensitized solar cell[J]. International journal of energy research, 2020,44(13):10846-10859.
doi: 10.1002/er.5752 URL |
[61] |
OTONI C G, AVENA-BUSTILLOS R J, AZEREDO H M C, et al. Recent advances on edible films based on fruits and vegetables-a review[J]. Comprehensive reviews in food science and food safety, 2017,16(5):1151-1169.
doi: 10.1111/1541-4337.12281 URL |
[62] |
ASHASSI-SORKHABI H, ES’HAGHI M. Corrosion inhibition of mild steel in hydrochloric acid by betanin as a green inhibitor[J]. Journal of solid state electrochemistry, 2009,13(8):1297-1301.
doi: 10.1007/s10008-008-0673-5 URL |
[63] |
TANAKA Y, SASAKI N, OHMIYX A. Biosynthesis of plant pigments anthocyanins, betalains and carotenoids[J]. The plant journal, 2008,54(4):733-749.
doi: 10.1111/j.1365-313X.2008.03447.x URL |
[64] |
JERZ G, SKOTZKI T, FIEGE K, et al. Separation of betalains from berries of phytolacca americana by ion-pair high-peed counter-current chromatography[J]. Journal of chromatography A, 2008,1190(1-2):63-73.
doi: 10.1016/j.chroma.2008.02.093 URL |
[65] |
GANDIA-HERRERO F, Garcia-Carmona F. Biosynthesis of betalains: yellow and violet plant pigments[J]. Trends in plant science, 2013,18(6):334-343.
doi: 10.1016/j.tplants.2013.01.003 URL |
[66] | 王长泉, 刘涛, 王宝山. 植物甜菜素研究进展[J]. 植物学通报, 2006,23(3):302-311. |
[1] | 刘艳萍, 王莹, 金永福, 李嘉欣, 孙唯玉, 赵俊杰, 薛刚. 皂荚皂苷的絮凝脱色及膜分离纯化[J]. 中国农学通报, 2022, 38(35): 1-6. |
[2] | 番华彩, 曾莉, 李卫雁, 丁云秀, 郭志祥, 李舒, 徐胜涛, 郑泗军, 王永斌. 香蕉细菌性软腐病防控药剂筛选及田间应用效果评价[J]. 中国农学通报, 2022, 38(31): 113-118. |
[3] | 崔雪娇, 佟潇禹, 张彦龙, 曾伟民. 刺五加果多糖ASPF的结构表征及其体外抗肺癌活性研究[J]. 中国农学通报, 2022, 38(22): 157-164. |
[4] | 骆美, 郭龙, 费坤, 张天恩, 李陈, 马友华. 耕地质量提升技术及其应用[J]. 中国农学通报, 2022, 38(21): 76-81. |
[5] | 秦荣秀, 杨漓, 李桂珍, 谷瑶. 广西多穗石柯根皮苷的提取及纯化工艺研究[J]. 中国农学通报, 2022, 38(16): 139-144. |
[6] | 李文业, 徐放, 王海华, 朱报著. ‘广东含笑’研究进展[J]. 中国农学通报, 2022, 38(14): 47-52. |
[7] | 刘亚昕, 闫桦, 唐玲, 谭文勃, 兴旺, 刘大丽. 红甜菜和甜菜红素的综合应用研究进展[J]. 中国农学通报, 2022, 38(13): 157-164. |
[8] | 陈芳玲, 樊娅萍, 贺苗苗, 王倡宪. 丛枝菌根真菌在药用植物上的应用研究进展[J]. 中国农学通报, 2022, 38(12): 55-60. |
[9] | 房佳敏, 马成瑶, 张彦龙. 黑木耳凝集素对RAW264.7细胞免疫调节作用的研究[J]. 中国农学通报, 2021, 37(9): 125-130. |
[10] | 李玮. 新型除草剂氟咯草酮在青海高原马铃薯田的应用及安全性[J]. 中国农学通报, 2021, 37(9): 149-154. |
[11] | 李岩, 付瑶, 陈勇, 魏政. 奶牛围产前期钙的稳态和负DCAD日粮的应用[J]. 中国农学通报, 2021, 37(35): 88-92. |
[12] | 巫文鑫, 李斌, 刘小勇, 刘佳莉, 李永华. 寄生茶研究现状与发展前景[J]. 中国农学通报, 2021, 37(34): 141-146. |
[13] | 刘明, 唐佳文, 沙爽, 孟剑侠, 程丽, 李冲伟. 基于分子蒸馏技术紫苏油α-亚麻酸纯化效果的研究[J]. 中国农学通报, 2021, 37(33): 135-141. |
[14] | 蔡守峰, 萧优平, 贺文员, 陈枫, 宋福强. 园林绿化垃圾资源化利用产品在园林绿化中的应用效果评价[J]. 中国农学通报, 2021, 37(29): 64-70. |
[15] | 张旭, 胡宝贵. 中国农业节水灌溉技术应用研究进展[J]. 中国农学通报, 2021, 37(26): 153-158. |
阅读次数 | ||||||
全文 |
|
|||||
摘要 |
|
|||||