甘蔗和斑茅Ⅲ型过氧化物酶基因（SoPOD-1和SaPOD-1）cDNA克隆与分析

doi:10.11924/j.issn.1000-6850.casb15070177

中国农学通报 ›› 2015, Vol. 31 ›› Issue (36): 139-144.doi: 10.11924/j.issn.1000-6850.casb15070177

所属专题：生物技术

甘蔗和斑茅Ⅲ型过氧化物酶基因（SoPOD-1和SaPOD-1）cDNA克隆与分析

胡小文,姚艳丽,邢淑莲,徐磊,刘洋

(中国热带农业科学院湛江实验站,广东湛江 524000）

收稿日期:2015-07-30 修回日期:2015-12-07 接受日期:2015-10-22 出版日期:2015-12-30 发布日期:2015-12-30
通讯作者: 刘洋
基金资助:
中央公益性科研基本业务费项目“割手密干旱胁迫相关Ⅲ型过氧化物酶(Prxs)基因的筛选与遗传分析”(1630062015025)，“植物Ⅲ型过氧化物酶(Prxs)基因序列进化规律研究”(zjky201505)。

Isolation and Characterization of Class III Peroxidases Gene (SoPOD-1 and SaPOD-1) cDNA in Saccharum officinarum and Saccharum arundinaceum

Hu Xiaowen, Yao Yanli, Xing Shulian, Xu Lei, Liu Yang

(Zhanjiang Experiment Station, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang Guangdong 524000)

Received:2015-07-30 Revised:2015-12-07 Accepted:2015-10-22 Online:2015-12-30 Published:2015-12-30

摘要/Abstract

摘要： 研究旨在通过克隆甘蔗和斑茅Ⅲ型过氧化物酶基因(Prxs)，分析比较2个物种Prxs基因差异，探讨2个物种Prxs功能差异的遗传基础。采用同源克隆方法，以高粱Prxs基因序列为探针，克隆获得甘蔗和斑茅Prxs基因。本研究从斑茅和甘蔗中成功克隆获得了4个Prxs基因cDNA序列，其中斑茅2个基因长度均为1083 bp，存在一个差异位点，甘蔗2个基因长度均为1084 bp，存在11个碱基差异。4个基因开放阅读框均为996 bp，编码331个氨基酸。蛋白质保守结构域分析表明，克隆获得的4个基因均具有Prxs典型保守结构域，为目标基因。系统进化分析表明，克隆获得的基因与高粱、玉米和水稻的同源基因相似性最高，在一些双子叶植物，甚至裸子植物也能找到其同源基因，且氨基酸序列的相似程度与物种分化的程度基本一致，可供植物分类作参考。该试验从斑茅和甘蔗中成功克隆获得了4个Prxs基因cDNA序列，为深入研究Prxs基因在不同植物中功能差异提供了一定的参考。

关键词: 木薯, 木薯, AGPase小亚基基因, 克隆, 5’端, 改良RACE方法

Abstract: The aim of this study was to investigate the difference of class Ⅲ peroxidases (Prxs) genes between Saccharum officinarum and Saccharum arundinaceum and explore the genetic basis of the functional differences of Prxs genes in these two species by cloning and analyzing their Prxs gene. A Sorghum bicolor Prxs gene was used as probe, by using the homologous clonal strategy, Prxs genes were cloned from Saccharum officinarum and Saccharum arundinaceum. In this study, four Prxs genes cDNA were obtained, both of the two Prxs genes cDNA length in Saccharum arundinaceum were 1083 bp, there was only one different site between them, another two Saccharum officinarum Prxs genes cDNA length were 1084 bp, which had eleven bases difference. The four cloned genes had a 996 bp open reading frame, encoding 331 amino acid. The protein conservative structural domain analysis showed that all of the four cloned genes had typical well-conserved amino-acid residues of Prxs, and they were the aimed genes. The phylogeny analysis indicated that the cloned genes had high similarity with homologous genes in Sorghum bicolor, zea maize and oryza sativa, also had relationship to the dicotyledon plants, or even to some gymnosperms, and the similarity degree of amino acid sequences were consistent with species divergence, which provided an evidence for plant classification. Four Prxs genes were cloned from Saccharum officinarum and Saccharum arundinaceum in this study, it provided a reference for further study in distinguishing the functional difference among different plants.

中图分类号:

Q781

胡小文,姚艳丽,邢淑莲,徐磊,刘洋. 甘蔗和斑茅Ⅲ型过氧化物酶基因（SoPOD-1和SaPOD-1）cDNA克隆与分析[J]. 中国农学通报, 2015, 31(36): 139-144.

Hu Xiaowen,Yao Yanli,Xing Shulian,Xu Lei and Liu Yang. Isolation and Characterization of Class III Peroxidases Gene (SoPOD-1 and SaPOD-1) cDNA in Saccharum officinarum and Saccharum arundinaceum[J]. Chinese Agricultural Science Bulletin, 2015, 31(36): 139-144.

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甘蔗和斑茅Ⅲ型过氧化物酶基因（SoPOD-1和SaPOD-1）cDNA克隆与分析

Isolation and Characterization of Class III Peroxidases Gene (SoPOD-1 and SaPOD-1) cDNA in Saccharum officinarum and Saccharum arundinaceum

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