体细胞克隆荷斯坦奶牛出生后死亡犊牛内脏器官的组织病理学观察

doi:10.11924/j.issn.1000-6850.casb20190500184

中国农学通报 ›› 2020, Vol. 36 ›› Issue (20): 114-118.doi: 10.11924/j.issn.1000-6850.casb20190500184

所属专题：生物技术；畜牧兽医

• 畜牧·动物医学·蚕·蜂 • 上一篇下一篇

体细胞克隆荷斯坦奶牛出生后死亡犊牛内脏器官的组织病理学观察

孙伟¹^,², 包向男¹, 乌云高娃¹, 王建国³, 李永胜¹, 李善铎¹, 张铁柱¹, 王飞飞¹, 李喜和¹^,⁴()

¹内蒙古赛科星繁育生物技术集团股份有限公司,呼和浩特 011517
²内蒙古大学省部共建草原家畜生殖调控与繁育国家重点实验室,呼和浩特 010070
³内蒙古大学哺乳动物生殖生物学及生物技术教育部重点实验室,呼和浩特 010021
⁴内蒙古大学生命科学学院蒙古高原动物遗传资源研究中心,呼和浩特 010070

收稿日期:2019-05-23 修回日期:2019-07-30 出版日期:2020-07-15 发布日期:2020-07-20
通讯作者: 李喜和
作者简介:孙伟,男,1979年出生,内蒙古乌兰察布卓资人,中级畜牧师,硕士,主要从事动物生殖生物学与生物技术方面的研究。通信地址：011517内蒙古自治区呼和浩特盛乐现代服务业集聚区金盛路与云谷大道东段交汇处内蒙古赛科星繁育生物技术集团股份有限公司,E-mail：swzyh769500@163.com。
基金资助:
内蒙古自治区“呼和浩特市科技计划项目”(2010-农-6)

The Internal Organs of Dead Calf of Somatic Cloning Holstein Cows: Histopathological Observation

Sun Wei¹^,², Bao Xiangnan¹, Wu Yungaowa¹, Wang Jianguo³, Li Yongsheng¹, Li Shanduo¹, Zhang Tiezhu¹, Wang Feifei¹, Li Xihe¹^,⁴()

¹Inner Mongolia Saikexing Reproductive Biotechnology Co., Ltd, Hohhot 011517
²The State key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot 010070
³The Key Laboratory of Mammalian Reproductive Biology and Biotechnology of the Ministry of Education, Inner Mongolia University, Hohhot 010021
⁴Research Center for Animal Genetic Resources of Mongolia Plateau, College of Life Sciences, Inner Mongolia University, Hohhot 010070

Received:2019-05-23 Revised:2019-07-30 Online:2020-07-15 Published:2020-07-20
Contact: Li Xihe

摘要/Abstract

摘要：

为探究体细胞克隆荷斯坦奶牛出生后死亡的原因,对新生后死亡的克隆荷斯坦公牛和自然繁殖的荷斯坦公犊的主要组织器官进行比较。通过解剖和石蜡切片-HE(hematoxylin-eosin staining)染色技术,对主要的组织器官结构进行观察和分析。结果表明,新生后死亡的克隆荷斯坦公牛肺部结构清晰;肝脏的肝细胞明显肿大,出现脂肪轻度变性;肾小管上皮细胞出现变性;心肌的肌纤维间空隙增大;骨骼肌纤维间隙明显,空泡变性,这可能导致该克隆牛犊肌肉无力并功能不全;淋巴结皮髓质界限不分明,淋巴小结细胞较稀疏,生发中心不明显,淋巴窦细胞较少;脾脏内红细胞较少,这说明该克隆牛的造血功能可能不完善;胸腺的皮质部分和髓质部分界限不明显,嗜酸性胸腺小体不易辨认,可能发育不全。该克隆公牛免疫器官出现不同程度的发育不全现象较严重,这有可能是其出生后死亡率高的主要原因。

关键词: 体细胞克隆, 器官发育异常, 组织病理学, 出生死亡, 荷斯坦奶牛

Abstract:

To investigate the death causes of cloned Holstein bulls, we compared major tissues and organs of the neonatal-death cloned holstein bull and natural propagation Holstein bull calf. We observed and analyzed the main tissues and organs by anatomy and HE (hematoxylin-eosin staining) staining. The results showed that the lung structure of the neonatal-death cloned Holstein bull was clear. Its liver cells were obviously swollen and the liver had slight degeneration. The renal tubular epithelial cells of the kidney had slight degeneration. The interstitial space between the myocardium fibers was enlarged. Skeletal muscle fiber gap was obvious, and vacuolar degeneration, which might lead to muscle weakness and insufficiency of the cloned bull. The boundaries of the lymphoid cortex and medulla were not distinct. Lymph node cells were sparse, germinal center was not obvious, and lymphatic sinus cells were less. There were fewer red blood cells in the spleen, which might indicate that the cloned bull had imperfect hematopoietic function. The boundary between the cortical and the medulla of the thymus was not obvious. The eosinophilic thymus gland was not easily identifiable and might be hypoplasia. The cloned Holstein bull’s immune organs had different degrees of hypoplasia, which might be the main reason for the high mortality after birth.

Key words: somatic cell cloning, organ abnormality, histopathology, neonatal death, Holstein cow

中图分类号:

孙伟, 包向男, 乌云高娃, 王建国, 李永胜, 李善铎, 张铁柱, 王飞飞, 李喜和. 体细胞克隆荷斯坦奶牛出生后死亡犊牛内脏器官的组织病理学观察[J]. 中国农学通报, 2020, 36(20): 114-118.

Sun Wei, Bao Xiangnan, Wu Yungaowa, Wang Jianguo, Li Yongsheng, Li Shanduo, Zhang Tiezhu, Wang Feifei, Li Xihe. The Internal Organs of Dead Calf of Somatic Cloning Holstein Cows: Histopathological Observation[J]. Chinese Agricultural Science Bulletin, 2020, 36(20): 114-118.

图/表 2

图1. 克隆犊牛重要组织器官病理组织学图片 a大脑(200×),b 肺(200×),c肝脏(200×),d睾丸(200×),e肌肉(200×),f淋巴结(200×),g脾脏(200×),h肾脏(200×),l小脑(200×),m心肌(200×),n胸腺(200×),o瓣胃(200×),p大肠(200×),q小肠(200×)

图2. 自然繁殖犊牛重要器官组织图片 a大脑(200×),b肺(200×),c肝脏(200×),d睾丸(200×),e肌肉(200×),f淋巴结(200×),g脾脏(200×),h肾脏(200×),l小脑(200×),m心肌(200×),n胸腺(200×),o瓣胃(200×),p大肠(200×),q小肠(200×)

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体细胞克隆荷斯坦奶牛出生后死亡犊牛内脏器官的组织病理学观察

The Internal Organs of Dead Calf of Somatic Cloning Holstein Cows: Histopathological Observation

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