中国荷斯坦奶牛TBX21基因与产奶性状及体细胞数的遗传效应分析

doi:10.11924/j.issn.1000-6850.casb15050173

中国农学通报 ›› 2015, Vol. 31 ›› Issue (26): 26-30.doi: 10.11924/j.issn.1000-6850.casb15050173

所属专题：生物技术；畜牧兽医

中国荷斯坦奶牛TBX21基因与产奶性状及体细胞数的遗传效应分析

孟洪¹,赵凤²,盛熙晖¹,齐晓龙¹,常迪¹,邢书涵¹,刘林²,李俊雅³,郭勇¹,倪和民¹

(¹北京农学院动物科技学院,北京 102206；²北京奶牛中心,北京 100192；³农科院北京畜牧兽医研究所,北京 100193）

收稿日期:2015-05-28 修回日期:2015-07-01 接受日期:2015-07-02 出版日期:2015-09-23 发布日期:2015-09-23
通讯作者: 倪和民
基金资助:
2013年度北京市教委北京市属高等学校创新团队建设与教师职业发展计划项目“体细胞转基因克隆肉牛新品系培育与利用”(PXM2013_014207_000067)；2015年度北京市奶牛产业技术体系北京市创新团队(5075237007/002)；2013年国家863科技支撑计划“奶牛养殖业节能减排关键技术研究与示范”(2013BAD21B01)。

Genetic Effects of TBX21 Gene on Milk Production Traits and SCC of Chinese Holstein Cows

Meng Hong¹, Zhao Feng², Sheng Xihui¹, Qi Xiaolong¹, Chang Di¹, Xing Shuhan¹, Liu Lin², Li Junya³, Guo Yong¹, Ni Hemin¹

(¹College of Animal Science and Technology, Beijing University of Agriculture, Beijing 102206; ²Beijing Dairy Cattle Center, Beijing 100192; ³Institute of Animal Sciences of CAAS, Beijing 100193)

Received:2015-05-28 Revised:2015-07-01 Accepted:2015-07-02 Online:2015-09-23 Published:2015-09-23

摘要/Abstract

摘要： 为鉴定与奶牛产奶性状和乳房炎抗性相关的遗传标记，为将来的分子育种提供理论基础，以383头中国荷斯坦母牛（均为2胎牛）为研究对象，利用直接测序法及飞行时间质谱法首次寻找TBX21基因5'和3'侧翼区的SNP位点，进而对这些多态性位点与中国荷斯坦牛4个泌乳性状（305天产奶量、乳脂率、乳蛋白率、乳糖率）和体细胞数进行关联分析。结果表明：在TBX21基因5'和3'侧翼区存在2个SNP位点，一个是在3'侧翼区1091 bp处A→G的突变，一个是3'侧翼区654 bp处A→C的突变；这2个位点均处于哈代-温伯格(Hardy-Weinberg)平衡状态。关联分析结果表明，rs207725545位点AA基因型个体的4种泌乳性状和体细胞数均高于CC型个体(P>0.05)；rs110420592位点GG基因型个体的产奶量和乳糖率高于AA型个体(P>0.05)，AA基因型个体的乳脂率和乳蛋白率高于GG型个体(P>0.05)，而GA基因型个体的体细胞数略高于其他2种基因型个体(P>0.05)。因此，在本试验群体中，TBX21基因的rs110420592和rs207725545两个位点与产奶性状和体细胞数无显著关联，后续需要扩大群体对TBX21基因的其他位点进行遗传效应分析。

关键词: 大肠杆菌, 大肠杆菌, 中草药, 抑制作用, 超微结构

Abstract: In order to identify the genetic markers associated with milk production and mastitis resistance, and provide theoretical basis for molecular breeding, 383 Chinese Holstein cows (all were the 2nd parity) were chosen as study animals. Direct sequencing and time of flight mass spectrometry were used to find polymorphisms in 5' and 3'-flanking region of TBX21 gene. Then association of the two polymorphic sites with the 4 milk production traits including milk yield, fat percentage, protein percentage and lactose percentage and SCC (somatic cell count) was analyzed. The results showed that two polymorphic sites were detected in 5' and 3'-flanking region of TBX21 gene. There were one A→G mutation at 1091 bp and one A→C mutation at 654 bp in 3'-flanking region. Both of them were in Hardy-Weinberg equilibrium. Association analysis showed that the 4 milk production traits and somatic cell count of rs207725545 loci AA genotype were higher than those of CC genotype (P>0.05). For rs110420592, milk yield and lactose percentage of GG genotype were higher than those of AA genotype (P>0.05), fat percentage and protein percentage of AA genotype were higher than those of GG genotype (P>0.05), and somatic cell count of GA genotype were higher than that of GG and AA genotype (P>0.05). The two loci of rs110420592 and rs207725545 in TBX21 gene had no significant association with the milk production traits and somatic cell count. Analysis of genetic effects on other polymorphic sites of TBX21 gene was needed in the future.

孟洪,赵凤,盛熙晖,齐晓龙,常迪,邢书涵,刘林,李俊雅,郭勇,倪和民. 中国荷斯坦奶牛TBX21基因与产奶性状及体细胞数的遗传效应分析[J]. 中国农学通报, 2015, 31(26): 26-30.

Meng Hong,Zhao Feng,Sheng Xihui,Qi Xiaolong,Chang Di,Xing Shuhan,Liu Lin,Li Junya,Guo Yong and Ni Hemin. Genetic Effects of TBX21 Gene on Milk Production Traits and SCC of Chinese Holstein Cows[J]. Chinese Agricultural Science Bulletin, 2015, 31(26): 26-30.

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中国荷斯坦奶牛TBX21基因与产奶性状及体细胞数的遗传效应分析

Genetic Effects of TBX21 Gene on Milk Production Traits and SCC of Chinese Holstein Cows

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