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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (26): 110-114.

所属专题： 生物技术； 资源与环境； 水产渔业

• 水产 渔业 • 上一篇    下一篇

低值水产品蛋白资源酶解产物的抗氧化性分析和评价

田宝玉 林丽萍 黄薇 蓝灿华 蔡婉玲 郭菁 黄建忠   

  • 收稿日期:2012-06-04 修回日期:2012-06-18 出版日期:2012-09-15 发布日期:2012-09-15
  • 基金资助:

    食线虫细菌侵染性蛋白酶的分子改造及其在线虫生防中的应用;水产品加工用蛋白酶基因资源的分子改造和DNA Shuffling

Antioxidant Analysis and Evaluation of Protease-digested Productions for two Kinds of Low-valuable Fish Proteins

  • Received:2012-06-04 Revised:2012-06-18 Online:2012-09-15 Published:2012-09-15

PDF (PC)

780

摘要：

为分析评价水产品蛋白酶解产物活性特性及合适水解蛋白酶类的选择。采用不同的商品化蛋白酶和枯草蛋白酶BPN对市场上常见的经济鱼类白鲢和彩云雕蛋白进行酶解，测定各种酶在水解过程中的水解度，并对酶解产物的抗氧化活性进行了比较分析。结果表明：蛋白酶在水解反应的前20 min内水解度迅速增加，20~120 min之间逐渐达到平衡。复合蛋白酶和水解蛋白酶具有较强的水解能力，其中水解蛋白酶对彩云雕2 h的水解产物最高可达15.92%。白鲢鱼枯草蛋白酶水解液的抗氧化活性最好，其DPPH清除能力为87.11%。不同蛋白酶水解不同的水产品蛋白产生的酶解产物活性肽成分具有明显的差异。

关键词: 阴沟肠杆菌NRRL B-414, 阴沟肠杆菌NRRL B-414

Abstract:

This experiment aimed to evaluate and study hydrolysis capacities of different commercial proteases on two kinds of low-valuable fish and activities of the resultant protease-digested productions. The results showed that the hydrolysis capacities of the tested proteases increased rapidly within first 20 min, and then began to a flatten curve out or declining in 2 hours. The complex protease dialogue showed the higher hydrolysis capacity on silver carp, and also with the biggest 15.92% hydrolysis degree of commercial hydrolysis protease on Red Tilapia. Hydrolysis products of subtilisin BPN on silver carp showed strongest antioxidant activity in all the enzyme-digested products, up to 87.11% ability to clear the DPPH. We concluded that the activities of the hydrolysis products were very different in different commercial proteases on two kinds of fish proteins.