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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (33): 103-106.

• 农学 农业基础科学 • 上一篇    下一篇

芽孢杆菌E-1的鉴定及其抑菌活性

汪钱龙 陈新春 谢春琼 姬广海 魏兰芳   

  • 收稿日期:2012-07-13 修回日期:2012-08-23 出版日期:2012-11-25 发布日期:2012-11-25

Identification and Antagonistic Activity of Bacillus subtilis E-1

  • Received:2012-07-13 Revised:2012-08-23 Online:2012-11-25 Published:2012-11-25

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摘要：

从云南昆明市郊不同地点紫茎泽兰根际分离细菌，经平板对峙培养筛选出一株抑菌能力较强的菌株，命名为E-1。通过菌株的培养性状、常规生理生化特性和16S rDNA序列测定及其系统发育学分析，鉴定为枯草芽孢杆菌(Bacillus subtilis)。该菌株的16S rDNA序列已在GenBank中注册，登录号为JN648100。对E-1与供试的5株植物病原细菌和5株病原真菌进行对峙试验。结果显示：E-1对土传病原细菌和真菌都有不同程度的拮抗作用，是较好开发潜能的生防菌株。

关键词: 可追溯性, 可追溯性

Abstract:

Antagonistic strain E-1of rhizobacteria against the fungi and bacterial pathogen were selected on NA and potato dextrose agar with many rhizobacteria isolated from the rhizosphere soil of Ageratina adenophora Sprengel from Kunming suburbs in Yunnan, respectively. Identification on basis of phenotypic and physiological and biochemical properties from biolog data indicating that strain E-1 homologized with which descripted for genus Bacillus. 16s rDNA determination and analysis was used for further identification, which showed that 16s rDNA sequence of strain E-1 shared 100% homologies with published sequence of Bacillus subtilis GZB from Genbank, and belonged to the same branch in phylogenetic tree. In vitro antagonistic assay showed strain E-1 could suppress the growth of five plant bacterial and five plant fungal pathogens. The results showed that strain E-1 had different level antagonistic activity to the fungi and bacterial pathogen. And maybe potential used for biological control of plant diseases.