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中国农学通报 ›› 2012, Vol. 28 ›› Issue (32): 89-93.

所属专题: 生物技术 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

苜蓿多糖对小鼠淋巴细胞增殖和NK细胞活性影响的研究

李敬双 刘英姿 唐雨顺 王云浩 吴宗澄 于洋   

  • 收稿日期:2012-08-31 修回日期:2012-09-22 出版日期:2012-11-15 发布日期:2012-11-15
  • 基金资助:

    辽宁省自然科学基金“苜蓿多糖对小鼠树突状细胞和巨噬细胞功能的调节作用”

Research of the Effect of Alfalfa Polysaccharides on Mice Spleen Lymphocytes Proliferation and NK Cell Activity

  • Received:2012-08-31 Revised:2012-09-22 Online:2012-11-15 Published:2012-11-15

摘要:

为了探讨苜蓿多糖对小鼠免疫功能的影响,从苜蓿中提取苜蓿多糖,通过体外苜蓿多糖对淋巴细胞增殖和NK细胞活性影响的研究,初步确定其免疫调节活性。采用MTT法,检测苜蓿多糖对正常Balb/c小鼠体外脾淋巴细胞增殖的影响和NK细胞对K562细胞杀伤活性的影响。结果表明:苜蓿多糖可以单一激活促进小鼠脾淋巴细胞的增殖,可以协同ConA刺激T细胞增殖,或协同LPS刺激B细胞增殖,提示苜蓿多糖能够调节机体的细胞免疫和体液免疫功能;苜蓿多糖可以显著地增强NK细胞对K562靶细胞的杀伤活性,提示苜蓿多糖能显著地增强机体非特异性免疫功能。因此,说明苜蓿多糖具有很好的免疫调节效果。

关键词: 产权改革, 产权改革

Abstract:

In order to discuss the effect of alfalfa polysaccharides on mice immuno function, alfalfa polysaccharides were extracted from alfalfa, and the research of how lymphopoiesis were affected by alfalfa polysaccharides were set up to determine its immunoregulatory activity. MMT assay were adopted to detect the effect of alfalfa polysaccharides on the proliferation of normal Balb/c mice spleen lymphocytes in vitro as well as the cytotoxicity NK cell to K562 cell. The results indicated that, alfalfa polysaccharides could be activated to enhance the proliferation of mice spleen lymphocytes singly and stimulate T lymphocyte proliferation cooperated with ConA or stimulate B lymphocyte cooperated with LPS, which pointed that the organism’s cellular immunity and humoral immunity function could be regulated by alfalfa polysaccharides; alfalfa polysaccharides could significantly strengthen the killing activity of NK cell to K526 target cell, which indicated that alfalfa polysaccharides could markedly boost organism’s nonspecific immuno function. Consequently, alfalfa polysaccharides possess impressive immuno regulatory function.

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