抗雌二醇单克隆抗体的制备与鉴定

中国农学通报 ›› 2005, Vol. 21 ›› Issue (6): 83-83.

抗雌二醇单克隆抗体的制备与鉴定

戴小华,,吴国娟,沈红,赵小萌,陆彦,

出版日期:2005-06-05 发布日期:2005-06-05

Production and Characterization of Anti-estradiol Monoclonal Antibody

Dai Xiaohua,, Wu Guojuan, Shen Hong, Zhao Xiaomeng, Lu Yan,

Online:2005-06-05 Published:2005-06-05

摘要/Abstract

摘要： 活化雌二醇（E2）C3处的酚-OH，与牛血清白蛋白（BSA）偶联，制备得E2的完全抗原，利用完全抗原免疫动物，采用单克隆抗体技术，经过细胞融合、克隆筛选、扩大培养、动物体内诱生腹水等过程，最后获得了E2的单克隆抗体，对纯化的单克隆抗体的性质鉴定，结果显示：其抗体效价为1:106，抗体属于IgG2a型；分子量为179000Da；E2单克隆抗体的亲和常数Kaff＝2.9×108L/mol。

Abstract: Estradiol (E2) was bound to bovine serum albumin (BSA) to form a complete antigen (E2-BSA), after the hydroxy (-OH) at the position of C3 was activated. A BALB/c mouse was immunized with the mixture of E2-BSA and Freund adjuvant. Monoclonal antibody-producing hybridoma anti-E2 was prepared by fusion of SP2/0 murine myeloma cells with spleen cell isolated from the immunized mouse. A monoclonal antibody (McAb) specific for E2 was produced in vivo by the hybridoma anti-E2. Analysis revealed that anti-E2 McAb (E2-McAb) was of the IgG2a type, with the molecular weight179000 daltons. The titer of E2-McAb was 1:106, and the affinity constant of constant of E2-McAb was 2.9×108L/mol.

戴小华,,吴国娟,沈红,赵小萌,陆彦,. 抗雌二醇单克隆抗体的制备与鉴定[J]. 中国农学通报, 2005, 21(6): 83-83.

Dai Xiaohua,, Wu Guojuan, Shen Hong, Zhao Xiaomeng, Lu Yan,. Production and Characterization of Anti-estradiol Monoclonal Antibody[J]. Chinese Agricultural Science Bulletin, 2005, 21(6): 83-83.