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中国农学通报 ›› 2005, Vol. 21 ›› Issue (6): 86-86.

所属专题: 油料作物 园艺

• 目次 • 上一篇    下一篇

甘蓝型油菜花茎高效再生体系研究

韩德俊,曹 莉,袁本威,陈耀锋,李振岐   

  • 出版日期:2005-06-05 发布日期:2005-06-05

Efficient Regeneration from Peduncle Segments of Brassica napus L.

Han Dejun, Cao Li, Yuan Benwei, Chen Yaofeng, Li Zhenqi   

  • Online:2005-06-05 Published:2005-06-05

摘要: 研究了甘蓝型油菜花茎外植体高效诱导不定芽的再生体系,结果表明:以含有2,4-D(0.5~1.0mg/L)的培养基对外植体进行预培养,以及在分化培养基中加入AgNO3(2~6mg/L),可显著降低外植体褐化坏死的频率,提高了不定芽的发生频率及其再生能力;6BA(2.5 mg/L)与NAA(0.1mg/L)配合使用有利于提高不定芽发生频率;再生的不定芽90%可长根

Abstract: Excised peduncle segments of three Brassica napus L. cultivars were used to optimize conditions for high-frequency shoot regeneration. The presence of 2,4-D(0.5~1.0 mg/L) in pre-cultured medium markedly enhanced the subsequent frequency of responding explants and the number of shoots/responding explant. The use of AgNO3 was a prerequisite for efficient shoot regeneration due to it strongly increased morphogenesis and decrease the frequency of explants death. BA (2.5mg/L) in combination with a low concentration of NAA(0.1mg/L) gave optimum shoot bud differentiation. The regenerated shoots could be rooted at a frequency of 95%.