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中国农学通报 ›› 2005, Vol. 21 ›› Issue (6): 89-89.

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培养基酸度对皂荚茎段试管培养生长的影响

兰彦平,顾万春,周连第,李淑英,曹庆昌   

  • 出版日期:2005-06-05 发布日期:2005-06-05

Effect of Medium Acidity on Growth of Gleditsia sinensis Growing in Vitro

Lan Yanping, Gu Wanchun, Zhou Liandi, Li Shuying, Cao Qingchang   

  • Online:2005-06-05 Published:2005-06-05

摘要: 研究了培养基酸度对皂荚茎段试管苗生长的影响,结果表明,在初始pH值为3.5或5.8的MS培养基中,皂荚茎段培养一定时间后,培养基pH值均有所下降,降低的幅度与接种的试管苗数目有关。初始pH值不同(3.5或5.8)的MS培养基中茎段的分化率间存在差异。

Abstract: Micropropagated Gleditsia sinensis was found to decrease the pH of Murashige and Skoog’s plant tissue culture medium (initial pH 3.5 or 5.8) to different values depending on the numbers of plants per tub. The difference was found in the rate of differentiation after plants had been grown on medium initially adjusted or buffered to values 3.5 or 5.8.