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中国农学通报 ›› 2013, Vol. 29 ›› Issue (13): 114-119.doi: 10.11924/j.issn.1000-6850.2012-3401

所属专题: 生物技术 园艺

• 林学 园艺 园林 • 上一篇    下一篇

单一及复合AM真菌初侵染对番茄苗的生理影响

唐超 李敏 刘永举 刘润进   

  • 收稿日期:2012-10-17 修回日期:2012-10-31 出版日期:2013-05-05 发布日期:2013-05-05
  • 基金资助:
    青岛科技局资助项目

Physiological Responds of Tomato Seedlings to Early Colonization by Single Strain and Compound Strains Arbucular Mycorrhizal Fungi

  • Received:2012-10-17 Revised:2012-10-31 Online:2013-05-05 Published:2013-05-05

摘要: 为明确丛枝菌根(arbuscular mycorrhizae,AM)真菌侵染番茄初期的生理生化动态变化趋势,于温室盆栽条件下设接种AM真菌摩西球囊霉(Glomus mosseae)侵染(S)、接种复合菌种[幼套球囊霉(Glomus etunicatum)+摩西球囊霉(Glomus mosseae)+地表球囊霉(Glomus versiforme)](M)和不接种对照(CK)。结果表明,复合菌种处理的番茄根系菌根侵染率、根长、根鲜重、叶片可溶性蛋白含量、叶片POD活性、叶片SOD活性显著高于单一菌株,而叶绿素含量则低于后者。受G. m+ G. e+ G. v复合菌株侵染的M组侵染率在约1个月时间内始终高于受单菌株G. m侵染的S组,AM真菌侵染8天内侵染率增长速率最大,8~20天之间出现缓慢增长的平台期,28天时侵染率出现下降趋势。由此可知,AM真菌侵染番茄的效应是多方面的,单菌株与复合菌株侵染效应有明显差异。关于复合菌剂与单一菌剂侵染番茄动态变化差异的研究,可以为综合开发利用复合菌剂、了解AM真菌相互作用提供一定的理论基础。

关键词: 广州市, 广州市

Abstract: The aim of the present research was to determine early physiological and biochemical dynamic change in tomato seedlings inoculated arbuscular mycorrhizal (AM) fungi. Under greenhouse conditions total 3 treatments were designed: inoculation with Glomus mosseae(S), inoculation with Glomus etunicatum, Glomus mosseae and Glomus versiforme(M), and non-inoculation control(CK). The results showed that the colonization percentage, root length, the root of tomatoes fresh weight, leaf soluble protein content, POD and SOD activity in leaves in M were significantly higher than that of S, while the leaf chlorophyll content was lower in the former than in the latter. By single strain G. m invaded and by the group S, G. m+ G. e+ G. v composite strains infect M to evaluate the effect of infecting significant difference between the time period for CK group in eight days after infection, and a certain degree of sustainability. Predictably, AM fungi of tomato effect was infecting many aspects, single strain and compound strains infect effect had obvious difference. It is suggested that the physiological responds of tomato seedlings to early colonization by arbucular mycorrhizal fungi should be different from the responds in middle or later growth period. This needed to be further studied.