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中国农学通报

中国农学通报 ›› 2013, Vol. 29 ›› Issue (7): 6-11.doi: 10.11924/j.issn.1000-6850.2012-3489

所属专题： 生物技术

• 林学 园艺 园林 • 上一篇    下一篇

黧蒴栲种源种子发芽的生理生化特性研究

宋倩 李志辉 童方平 刘振华 李贵 陈瑞   

  • 收稿日期:2012-10-27 修回日期:2012-11-16 出版日期:2013-03-05 发布日期:2013-03-05
  • 基金资助:
    国家林业公益专项 “高热能固体燃料树种新品种选育技术研究” (200904036)。

Physiological and Biochemical Characteristics in Seed Germination of Castanopsis fissa Rehd.et Wils Provenance

li chihui   

  • Received:2012-10-27 Revised:2012-11-16 Online:2013-03-05 Published:2013-03-05

PDF (PC)

603

摘要： 为了研究黧蒴栲种子发芽的生理生化特性，以7个种源地的黧蒴栲种子为试验材料，研究其千粒重、发芽率和一系列酶活性在种源间的差异，为种源选择与遗传资源保护提供理论依据。结果表明，黧蒴栲种子千粒重、发芽率和一系列酶活性在种源间差异显著，7个种源地种子发芽率最高的为通道县，达92.0%；最低为平果县，仅85.1%；且种子发芽率与种源地年降水量、种子千粒重、抗氧化酶保护活性、多酚氧化酶活性呈显著正相关，种子发芽率与种源地年均温、种子丙二醛含量则呈显著负相关。因此，通过黧蒴栲种子发芽率与各项生理指标间的相关关系，利用种子酶活性可判断种子发芽率。

关键词: 生物碱, 生物碱

Abstract: In order to study the physiological and biochemical characteristics in seed germination of Castanopsis fissa Rehd.et Wils, with seven provenances’seeds of Castanopsis fissa Rehd.et Wils as the test materials, the author studied the differences of grain-weight, germination rate and a series of enzyme activity between provenances, which was providing a basis theory in provenance selection and genetic resources protection. The results showed that: there was significant difference in seed germination rate, grain-weight and a series of enzyme activity between provenances and that the Tongdao County presented the highest seed germination rate (92.0% ) and the Pingguo County appeared the lowest seed germination rate (85.1% ). Seed germination percentage was related the significant positive correlation in annual precipitation, grain-weight, antioxidant enzymes protection activity and PPO activity had a related the significant negative correlation in annual average temperature and the content of MDA. Therefore, depending on the seed germination rate and the physiological parameter, people could estimate the seed germinate from the liveness of enzyme.