Abstract: The optimization of high-yield cultural practices under the system of rice (Oryza sativa L.) intensification was carried out in farmer’s field at Xianju in Zhejiang, used a super hybrid rice (Liangyoupeijiu) as material on orthogonal design (three factors and three levels) in the experiment. The results indicated the effects of major cultural practices of transplanting seedling, transplanting density, chemical N fertilizer input on the grain yield and its components significantly. The optimization of high-yield cultural practice under the system of rice intensification was: seedling of 15d from sowing date to transplant, transplanting density of 150000 hills/hm2, and total N application of 150kg/hm2 under basic fertilizer of 30000kg/hm2 green manure.