Abstract: Wanmai38, a strong gluten wheat, was taken as an experimental material to study the effect of soil basic fertility and nitrogen application methods on grain yield and quality. The result showed that the correlation between the application rate of N-fertilizer, the proportion of topdressing nitrogen and the grain yield were quadric curved lines; and the correlation between the application rate of N-fertilizer, the proportion of topdressing nitrogen and protein content, wet gluten content were significantly positive on the range from 0 to 300 kg/hm2 of N application. To get the highest yield, the nitrogen application methods of applying 238.6kg/hm2, and 35.1% of the topdressing in jointing in field one (lower soil fertility foundation), and the nitrogen application methods of applying 274.2 kg/hm2, and 47.2% the topdressing in jointing or in jointing and booting in field two (higher soil fertility foundation). The key to techniques for strong gluten wheat cultivation with high yield, good quality was to apply N-fertilizer properly on higher soil fertility foundation