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中国农学通报

中国农学通报 ›› 2014, Vol. 30 ›› Issue (4): 137-143.doi: 10.11924/j.issn.1000-6850.2013-1625

• 林学 园艺 园林 • 上一篇    下一篇

基于SSR标记的肇庆地区柑橘品种分类地位研究

郭雁君 吉前华   

  • 收稿日期:2013-06-13 修回日期:2013-07-23 出版日期:2014-02-05 发布日期:2014-02-05
  • 基金资助:
    广东省科技计划项目 “肇庆柑橘专业镇创新联盟建设” (2010B080201006)。

Classification of Zhaoqing Local Citrus Germplasm Resources based on Simple Sequence Repeat Molecular Marker Analysis

  • Received:2013-06-13 Revised:2013-07-23 Online:2014-02-05 Published:2014-02-05

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摘要： 评价广东省肇庆地区地方柑橘品种与其它部分柑橘属和近缘属植物的亲缘关系，为其分类和育种提供理论依据。从34对柑橘SSR引物中筛选到13对多态性较好的SSR引物，对8份肇庆地方柑橘品种和22份柑橘属、枳属、酒饼簕属植物材料进行PCR扩增，并采用1.5%琼脂糖凝胶电泳检测，用遗传相似性和聚类分析等方法分析检测结果。13对引物共扩增出83条谱带，多态检出率为100%，通过聚类分析能够有效的区分柑橘属、枳属和酒饼簕属，以及柑橘属内的种、品种甚至品系。结果表明SSR分子标记技术能够用于肇庆柑橘种质资源分类地位研究，并且供试的肇庆地方柑橘品种亲缘关系较近。

关键词: 地理分布, 地理分布

Abstract: Evaluation of genetic relationship between Zhaoqing local citrus germplasm resources and other citrus cultivars and related genera by simple sequence repeat (SSR) molecular marker technique, the results of present study may provide solid supporting evidence for their classification and breeding.The materials include main citrus cultivars planted in this region (8 samples), other Citrus cultivars (20 samples), Poncirus (1 sample), and Atalantia (1 sample, used as out-group) for PCR amplification with 13 pairs of SSR primers, the amplification product was tested in 1×TBE buffer solution using 1.5% agarose gel electrophoresis. Cluster analysis was performed using UPGMA, and a cluster tree was built using the NTSYSpc2.1 analysis software. Meanwhile, the genetic similarity coefficient was calculated. A total of 13 primer pairs with high polymorphism were selected from the 34 pairs of SSR. The codes of primer sequence with polymorphism are TAA3，CTT01，TAA15，SS18，TAA1，SS16，CMS30，CAC19，CMS24，CSSR036，CMS14，CMS21，CMS20. A total of 83 polymorphic bands were amplified. Each primer has an average of 6.4 polymorphic bands. The average polymorphism is 100%. The clustering results show that the SSR molecular marker technique can reveal genetic diversity and differentiate citrus, Poncitrus, and Atalantia genera from one another. This method is proven effective in identifying citrus species, breeds, and strains in this region. SSR molecular marker technique can effectively differentiate Zhaoqing local citrus germplasm resources . Tested materials showed a closer genetic relationship.