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中国农学通报 ›› 2005, Vol. 21 ›› Issue (7): 66-66.

所属专题: 园艺

• 目次 • 上一篇    下一篇

遗传标记及其在园艺植物研究中的应用

陈东明   

  • 出版日期:2005-07-05 发布日期:2005-07-05

Genetic Markers and Their Application in Gardening Plants′ Studies

Chen Dongming,   

  • Online:2005-07-05 Published:2005-07-05

摘要: 评述了形态学标记(morphological markers)、细胞学标记(cytological markers)、生化标记(biochemical markers)、DNA分子标记(DNA molecular markers)等技术的发展现状,着重介绍了近年来DNA指纹图谱技术,即限制性片段长度多态性(Restriction Fragment Length Polymorphism,RFLP)、随机扩增片段长度多态性 (Random Amplified Polymophismic DNA,RAPD)、简单重复序列 (simple sequence repeats,SSR)、扩增片段长度多态性(Amplified Fragment Length Polymorphism,AFLP)、单核苷酸多态性 (single nucleotide polymorphism,SNP) 等标记技术的特点及其在园艺植物品种的分类鉴定、纯度的分析检测及品种间亲缘关系等研究中的应用。

Abstract: The evolutive status of morphological markers, cytological markers, biochemical markers and DNA molecular markers were summarized in the article. This paper mainly introduce DNA fingerprint techniques such as restriction fragment length polymorphism, random amplified polymophismic DNA, simple sequence repeats, amplified fragment length polymorphism, single nucleotide polymorphism and so on. This paper also studies the application of the classification and indentification of these techniques, the analysis and detection of the cultivars’ purity and the relationship among the cultivars on garden plants.