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中国农学通报

中国农学通报 ›› 2005, Vol. 21 ›› Issue (7): 79-79.

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多因子正交试验对甜叶菊丛生芽诱导条件的筛选

赫福霞,于 晶,李茫雪,郝再彬,周进兴,苍 晶   

  • 出版日期:2005-07-05 发布日期:2005-07-05

Selection of Caespitose Shoots of Stevia Rebaudiana Bertoni using Orthogonal Experimental Design

He Fuxia, Yu Jing, Li Mangxue, Hao Zaibin Cang Jing   

  • Online:2005-07-05 Published:2005-07-05

摘要： 利用正交试验设计方法探讨了植物生长物质（6-苄基腺嘌呤、奈乙酸）、蔗糖、水解酪蛋白（CH）对甜叶菊丛生芽诱导的影响。结果表明：蔗糖对丛生芽诱导影响最大，其次是6-BA，NAA、CH影响较小。筛选出甜叶菊丛生芽诱导的最适培养基为MS + 6-BA 1mg/L+ 琼脂6g/L + 蔗糖30g/L。继代培养采用MS + 6-BA 0.5mg/L+ NAA0.05mg/L + 琼脂6g/L + 蔗糖30g/L。生根最适培养基为1/4MS + 0.1mg/L IBA +琼脂6g/L + 蔗糖30g/L +1g/L活性炭，生根率达100%。已获得驯化移栽成活的植株,移栽成活率为92.13﹪。

Abstract: Studies on the effect of plant hormones including 6-BA、NAA、and sucrose、CH on inducing Stevia Rebaudiana Bertoni’s Poly shoots using orthogonal experimental design. The results showed that the most important factor affecting on inducing caespitose shoots is sucrose, next is 6-BA、NAA, the lest one is CH. The best medium inducing poly shoots is MS+6-BA 1mg/L+ Agar6g/L+Sucrose30g/L. The developed medium is MS+6-BA 0.5mg/L+NAA0.05mg/L +Agar6g/L+Sucrose30g/L.The root shoots medium is 1/4MS+IBA 0.1mg/L+Agar6g/L +Sucrose 30g/L+active carbon1g/L, the rate of shooting root reach 100%. Now we have obtained many regeneration plantlets.