扩张蛋白(Expansin)研究进展

摘要/Abstract

摘要： 扩张蛋白是一类细胞壁酶蛋白，能够通过打断细胞壁多聚物之间的氢键诱导酸依赖的细胞壁延展和压力松弛。其由两类多基因家族编码，分别命名为α-expansin、β-expansin，每类扩张蛋白基因家族又有很多的基因成员。扩张蛋白已在不同的种属植物中被鉴定存在，并且大量的扩张蛋白基因被克隆。研究表明：每一个扩张蛋白基因在高度特定的位点和高度特定的细胞类型中表达，其表达在时间、空间上受到严格的调控且受到激素和环境因子的调控。目前认为扩张蛋白几乎参与了植物的整个发育过程：除具有增大细胞的功能外，扩张蛋白在细胞生长、种子萌发、根毛形成、根系生长、叶原基形成、叶子生长发育、果实成熟、器官脱离，以及花粉管生长方面起着重要的作用。就此最新研究进展，作一综合阐述。

Abstract: Expansins are cell wall proteins that induce pH dependent wall extension and stress relaxation by weakening the non-covalent interactions. It has been realized that expansins belong to two protein families, the alpha-and beta-expansins, Expansin proteins have so far been identified in most plants and most expansin genes have been cloned.The research shows that every expansin expresses in high special location and high special cell And the expression of every expansin is regulated strictly in development time and space and is regulated positivly by hormone and environments factors. It has been proposed that expansins have rather diverse effects throughout the whole developmental process. Besides playing an apparently key role in wall expansion, expansins have been implicated in an increasing number of processes including cell enlargement, seed germination, root hair formation, root growth, leaf primordium initiation,leaf growth, softening of fruits, fruit abscission and growth of the pollen tube of grasses through the stigma and the style. In this article, we expatiate the research proceeding on expansin all-roundly.

高英,王学臣. 扩张蛋白(Expansin)研究进展[J]. 中国农学通报, 2005, 21(7): 82-82.

Gao ying, Wang Xuechen. The Review of the Development of Researches on Expansin[J]. Chinese Agricultural Science Bulletin, 2005, 21(7): 82-82.

参考文献

1 Cosgrove D J. Expansive growth of plant cell walls.Plant Physiol. and bioch.,2000,38(1~2):109~124
2 Cosgrove D J. Loosening of plant cell walls by Expansins. Nature,2000,407:321~326
3 McQueen-Mason S J, Durachko D M,Cosgrove D J. Two endogenous proteins that induce cell wall extension in plant.Plant Cell,1992,4:1425~1433
4 Wang P, Flores H E,Humphrey A E. Production of expansin from light/dark growing Trichosanthes kirilowiivar. Japonicum root cultures.Biotechnol.Lett.,1994,16(9):955~958
5 Cho H T, Kende H. Tissue localization of expansin in deepwater rice.Plant Journal,1998,15(6):805~812
6 Shimizu Y, Aotsuka S, Hasegawa O, et al. Changes in levels of mRNAs for cell wall-related enzymesin growing cotton fiber cells. Plant and Cell Physiol.,1997,38(3):375~378
7 李连朝.植物细胞壁扩张蛋白的研究:[博士学位论文].北京:中国农业大学,1997.61~67
8 Lee D K, Ahn J H,Song S K, et al. Expression of an expansin gene is correlated with root elongation in soybean. Plant Physiology,2003, 131:.985~997
9 Cosgrove D J. New genes and new biological roles for expansins.Curr.Opin.Plant Biology, 2000, 3(1):73~78
10 Cosgrove D J.Enzymes and other agents that enhance cell wall extensibility. Annual. Review of Plant Physiol. and Plant Mol. Biol. ,1999,50:391~417
11 Yi Li, et al. Plant Expansins Are a Complex Multigene Family with an Ancient Evolutionary Origin.Plant Physiology, 2002, 128: 854~864
12 Cho H T, Kende H T.Tissue localization of Expansins in deepwater rice. Plant Journal, 1998,15: 805~812
13 Rose J, Lee K C, Bennett H H. Expression of a divergent expansin gene is fruit-specific and ripening-regulated. Proc.Natl.Acad.Sci.U.S.A., 1997,94(11):5955~5960
14 Lee D K, et al. Expression of an Expansin Gene Is Correlated with Root Elongation in Soybean.Plant Physiology,2003,131:985~997
15 Hutchison K W, Singer P B, McInnis S, et al. Expansins are conserved in conifers and expressed in hypocotyls in response to exogenous auxin. Plant Physiology, 1999,120(3):827~831
16 Vriezen W H, et al.Submergence induces expansin gene expression in flooding-tolerant Rumex palustris and not in flooding-intolerant R. acetosa. Planta ,2000,210: 956-963
17 Cho H T, Kende H. Expression of Expansin Genes is Correlated with Growth in Deepwater Rice. The Plant Cell, 1997 ,9:1661~1671
18 Wu Y.,Sharp R E,Cosgrove D J.Growth maintenance of the maize primary root at low water potentials involves increases in cell extension properties Expansins activity and wall susceptibility to Expansins. Plant Physiology,1996,111:765~772
19 Choi D,et al.Regulation of Expansin Gene Expression Affects Growth and Development in Transgenic rice Plants.The Plant Cell ,2003,15:1386~1398.
20 Feng Chen, Kent J B.Expression of an Expansin Is Associated with Endosperm Weakening during Tomato Seed Germination. Plant Physiology, 2000,124:1265~1274
21 Cho H T,et al. Regulation of Root Hair Initiation and Expansin Gene.The Plant Cell, 2002,14:3237~325322 Stephane Pien, et al. Local expression of expansin induces the entire process of leaf development and modifies leaf shape. Proc.Natl.Acad.Sci.U.S.A. ,2001 ,98 : 11812~11817
23 Cho H T,et al.Altered expression of expansin modulates leaf growth and pedicel abscission in Arabidopsis thaliana. Proc.Natl.Acad.Sci.U.S.A.,2000 ,97 ( 17 ):9783~978
24 Brummoll D A, Harpster M H, Dunsmuir P. Differential expression of expansin gene family members during growth and ripening of tomato fruit. Plant Mol. Biol.,1999,39(1):161~169
25 Brummel D A, et al. Modification of Expansin Protein Abundance in Tomato Fruit Alters Softening and Cell Wall Polymer Metabolism during Ripening. The Plant Cell, 1999,11: 2203~2216
26 Civello P M, Powell A L T, Sabehat A, et al. An expansin gene expressed in ripening strawberry fruit. Plant Physiology, 1999,121 (4):1273-1279.
27 Cosgrove D J. Creeping walls,softening fruit,and penetrating pollen tubes:the growing roles of expansins.Proc.Natl.Acad.Sci.U.S.A.,1997,94(11):5504~5505
28 Cosgrove D J, Bedinger P, Durachko D M.Group I allergens of grass pollen as cell wall-loosening agents.Proc.Natl.Acad.Sci.U.S.A .,1997,94(12):655~6564