Abstract: Content of crude protein and 18 amino acid of 9 kinds of Euonymus fortunei were measured and analysed. The results showed that the leaves of Euonymus fortunei have higher content of crude protein, many kinds of amino acid, and 8 kinds necessary amino acid in animal’s body. The contents of crude protein and amino acid among varieties were extremely remarkable. The difference of the contents of different amino acid that belong to same variety were extremely remarkable. Contents of glutamic acid is the highest in 18 amino acids and cystine, tryptophan and ammonia were lower than elso and relatively stable. But they were positively correlated with the contents of crude protein, and the difference between them was not remarkable. The else 16 kinds of amino acid were positively correlated with the contents of crude protein and reached an extremely remarkable level. So authors draw a conclusion on the traits of crude protein and amino acids in leaves have homegeneity and suggest to develop researching of pasture, feedstuff and health tea in Euonymus fortunei.