Abstract: Through developing HPLC on studying betaine content of Lycium barbarum in different regions using the Lycium barbarum material the betaine in content of Lycium barbarum was contrasted in different regions in July 2004 .The results showed the difference of betaine content of Lycium barbarum planted in Ningxia regions by analyzing the soil physics and chemistry factors and betaine content of Lycium barbarum in four main regions of Ningxia .The relations between soil factors and betaine of Lycium barbarum was discussed, The results showed that the correlation coefficient between betaine in different regions was significant ,the betaine content in old producing area-- Zhongning was more than that in Huinong .The correlation coefficient between betaine and soil factors was not significant ,but it presented positive correlation against efficient P，K. Betaine content in the fruit of Lycium barbarum L in Ningxia invariably presented positive correlation to the salt content of the earth, in which it showed markable .