GDF9与BMP15在绵羊腔前卵泡及卵母细胞体外成熟过程中的差异表达

doi:10.11924/j.issn.1000-6850.2014-0786

中国农学通报 ›› 2014, Vol. 30 ›› Issue (35): 35-41.doi: 10.11924/j.issn.1000-6850.2014-0786

所属专题：生物技术；畜牧兽医

GDF9与BMP15在绵羊腔前卵泡及卵母细胞体外成熟过程中的差异表达

张帆

北京农学院动物科学技术学院

收稿日期:2014-03-21 修回日期:2014-03-21 接受日期:2014-06-17 出版日期:2015-03-18 发布日期:2015-03-18
通讯作者: 张帆
基金资助:
2013年度北京市教委北京市属高等学校创新团队建设与教师职业发展计划项目“体细胞转基因克隆肉牛新品系培育与利用”(PXM2013_ 014207_000067)；2013年度国家自然基金面上项目“牛体内外植入前胚胎IFNτ的差异表达及对子宫上皮细胞ISG15和Wnt7a表达的体外诱导”(31272526)；2011年度国家自然基金面上项目“不同来源植入前期绵羊胚胎IFNτ差异表达及其体外诱导子宫上皮细胞Galectin15表达的研究”(31072185)。

The Expressional Differences of Growth Differentiation Factor 9 and Bone Morphogenetic Protein 15 on sheep pre-antral follicles and their oocytes matured in vitro

Received:2014-03-21 Revised:2014-03-21 Accepted:2014-06-17 Online:2015-03-18 Published:2015-03-18

摘要/Abstract

摘要： 为了探讨GDF9与BMP15在绵羊腔前卵泡发育及卵母细胞体外成熟过程中的作用，运用腔前卵泡的显微分离方法和RT-PCR技术，检测了GDF9、BMP15 mRNA在绵羊腔前卵泡及卵母细胞体外成熟过程中(0、8、16、24 h)的相对表达丰度。结果表明：绵羊COCs在体外培养的不同时期，卵丘细胞扩展呈现不同的形态学变化；腔前卵泡中GDF9、BMP15 mRNA表达量均低于体外成熟不同时期卵母细胞，且腔前卵泡中GDF9 mRNA表达量显著低于体外成熟不同时期卵母细胞。揭示GDF9和BMP15对卵巢卵泡发育和卵母细胞体外成熟发挥重要作用，在绵羊卵母细胞体外成熟过程中，这2个因子可能是独立或协同地影响卵丘细胞增殖扩散。

关键词: 信息服务, 信息服务

Abstract: The study aimed to investigate the expressional changes of GDF9 and BMP15 on sheep follicle development and their in vitro matured oocytes. The micro-dissected technique was applied to isolate ovine pre-antral follicles. The expression of GDF9 and BMP15 on their pre-antral follicles and oocytes matured in vitro (0, 8, 16 and 24 h) were detected respectively by RT-PCR. The results showed that the cumulus cell expansion presented different alterations in morphology during vitro maturation of sheep COCs. GDF9 and BMP15 mRNA levels detected in pre-antral follicles were lower than those oocytes of different developmental stages as well as in vitro maturation. There were significantly transcriptional differences of GDF9 between preantral follicles and those different developing stages of oocytes, which suggested that GDF9 and BMP15 play an essential role during follicle development and in vitro maturation of sheep oocytes. All of these implied these two factors may independently or collectively affect the granulose cell proliferation and cumulus expansion.

张帆,倪和民,马欣,杨洪武,刘云海,郭勇,杨佐君. GDF9与BMP15在绵羊腔前卵泡及卵母细胞体外成熟过程中的差异表达[J]. 中国农学通报, 2014, 30(35): 35-41.

倪和民, 马欣, 杨洪武, 刘云海, 郭勇 and 杨佐君. The Expressional Differences of Growth Differentiation Factor 9 and Bone Morphogenetic Protein 15 on sheep pre-antral follicles and their oocytes matured in vitro[J]. Chinese Agricultural Science Bulletin, 2014, 30(35): 35-41.

参考文献

[1] Findlay JK, Drummond AE, Dyson ML, et al. Recruitment and development of the follicle: the roles of the transforming growth factor-b superfamily [J]. Mol Cell Endocrinol, 2002,191(1):35-43.
[2] Gilchrist RB, Lane M, Thompson JG. Oocyte- secreted factors: regulators of cumulus cell function and oocyte quality [J]. Hum Reprod Update, 2008,14(2):159-177.
[3] Knight PG, Glister C. Local roles of TGF- beta superfamily members in the control of ovarian follicle development [J]. Anim Reprod Sci,2003,78(3):165-183.
[4] Lin SY, Morrison JR, Phillips DJ, et al. Regulation of ovarian function by the TGF-b superfamily and follistatin [J]. Reproduction, 2003,126(1):133-148.
[5] 潘慧慧,黄荷凤.生长分化因子9、骨形态发生蛋白15信号转导机制研究进展[J].国际生殖健康/计划生育杂志,2009,28(3):162-165.
[6] 李碧侠,储明星,王金玉.生长分化因子9基因的研究进展[J].中国畜牧兽医,2002,29(6):33-36.
[7] Duffy DM. Growth differentiation factor- 9 is expressed by the primate follicle throughout the periovulatory interval [J]. Biology of Reproduction,2003,69(2):725-732．
[8] Dong J, Albertini DF, Nishimori K, et al. Growth differentiation factor-9 is required during early ovarian folliculogenesis [J]. Nature, 1996,383(6600):531-535.
[9] Galloway SM, McNatty KP, Cambridge LM, et al. Mutations in an oocyte- derived growth factor gene (BMP15) cause increased ovulation rate and infertility in a dosage- sensitive manner[J]. Nature Genetics,2000,25(3):279-283.
[10] 董传河,杜立新.BMP15对卵巢功能的调节及对生殖的影响[J]. 动物学杂志,2008,43(4):140-145.
[11] Parrott J A, Skinner M K. Kit ligand actions on ovarian stromal cells: effects on theca cell recruitment and steroid production [J]. Molecular Reproduction and Development, 2000, 55(1): 55 -64.
[12] Otsuka F, Shimasaki S. A negative feedback system between oocyte bone morphogenetic protein 15 and granulosa cell kit ligand: its role in regulating granulose cellmitosis [J]. PNAS,2002,99(12): 8060-8065.
[13] Shimasaki S, Moore R K, Otsuka F, et al. The bone morphogenetic protein system in mammalian reproduction [J]. Endocrine Reviews, 2004,25(1):72-101.
[14] 董传河,杜立新.山东地方山羊BMP15基因多态性与产羔数性状关联分析[J].动物学杂志,2010,45(1):58-69.
[15] 常迪,崔旭,高雅等.不同分离方法及培养方式对绵羊腔前卵泡发育能力的影响[C].见:中国畜牧兽医学会论文集编写组.中国畜牧兽医学会动物繁殖学分会第十五届学术研讨会论文集.北京:中国畜牧兽医学会编辑部,2010:12-16.
[16] 姚桂东,陶勇,等.哺乳动物腔前卵泡培养方法研究进展[J].中国畜牧兽医,2007,34(1):58-61.
[17] 沈霞芬.家畜组织学与胚胎学[M].第3版.北京:中国农业出版社, 2005:202-207.
[18] Prochazka R, Nemcova L, Nagyova E, et al. Expression of growth differentiation factor 9 messenger RNA in porcine growing and preovulatoy ovarian follicles [J]. Biol Reprod,2004,71(4):1290- 1295.
[19] 梁洋,刘娣,孙兴参,等.哺乳动物腔前卵泡体外培养及研究进展[J].动物医学进展,2005,26(3):32-35.
[20] 张耀君,王昭辉,文瑞丽等.家畜类腔前卵泡体外分离方法及其影响因素研究进展[J].四川动物,2009,28(6):949-951.
[21] 欧阳效晴,杨淑青,张春强,等.生长分化因子９在牛卵丘卵母细胞复合体体外成熟过程中的表达[J].畜牧兽医学报,2013,44(10): 1569-1575.
[22] Amar Nath, Veena Sharma, Pawan K. Dubey, et al. Impact of gonadotropin supplementation on the expression of germ cell marker genes (MATER, ZAR1, GDF9, and BMP15) during in vitro maturation of buffalo (Bubalus bubalis) oocyte [J]. In Vitro Cell. Dev. Biol.-Animal,2013,49(1):34–41.
[23] 管峰,艾君涛,庞训胜等.绵羊GDF9和BMP15基因多态性检测[J].生命科学研究,2005,9(2):184-188.
[24] Jia Peng, Qinglei Li, Karen Wigglesworth, et al. Growth differentiation factor 9:bone morphogenetic protein 15 heterodimers are potent regulators of ovarian functions[J].Current Issue, 2013,110 (8):776-785.
[25] Nisson E E, Skinner M K. Growth differentiation factor 9 stimulate progresson of early primary but not primordial rat ovarian follicle development [J]. Biol Reprod, 2002,67(3):1018-1024.
[26] Fenwick M A, Mora J M, Mansour Y T, et al. Investigations of transforming growth factor beta (TGF- β) signalling in preantral follicles of female mice reveal differential roles for bone morphogenetic protein 15 (BMP15) [J]. Endocrinology, 2013,154 (9):3011-3025
[27] Fenwick M A, Mansour Y T, Franks S, et al. Identification and regulation of bone morphogenetic protein antagonists associated with preantral follicle development in the ovary [J]. Endocrinology, 2011,152(9):3515-3526.
[28] Vogt J, Traynor R, Sapkota G P. The specificities of small molecule inhibitors of the TGFss and BMP pathways [J]. Cell Signal, 2011,23 (11):1831-1842.
[29] Miyoshi T, Otsuka F, Nakamura E, et al. Regulatory role of kit ligand-c-kit interaction and oocyte factors in steroidogenesis by rat granulose cells [J]. Mol Cell Endocrinol,2012,358(1):18-26.
[30] Hou-Kuan Li, Ting-Yung Kuo, Hsiu-Shan Yang, et al. Differential gene expression of bone morphogenetic protein 15 and growth differentiation facter 9 during in vitro maturation of porcine oocytes and early embryos [J]. Anim Reprod Sci,2008,103(3/4):312-322.
[31] Funda Gode, Bulent Gulekli, Erbil Dogan, et al.Influence of follicular fluid GDF9 and BMP15 on embryo quality[J].Fertility and Sterility,2011,95(7):2274-2278.

GDF9与BMP15在绵羊腔前卵泡及卵母细胞体外成熟过程中的差异表达

The Expressional Differences of Growth Differentiation Factor 9 and Bone Morphogenetic Protein 15 on sheep pre-antral follicles and their oocytes matured in vitro

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

关于本刊

作者中心

审者中心

在线期刊

联系我们

[1]	陈秋菲, 贾国强. 农业科学普及助力乡村振兴实证研究——以辽宁12316“三农”综合信息服务平台为例[J]. 中国农学通报, 2022, 38(11): 160-164.
[2]	侯瑞霞,孙伟,曹姗姗,唐小明. 大数据环境下林业资源信息云服务体系架构[J]. 中国农学通报, 2016, 32(2): 170-179.
[3]	赵轶洁,孟宪学,潘淑敏. 优化服务视角下的农科研究生英语学习用户研究[J]. 中国农学通报, 2016, 32(19): 199-204.
[4]	张永强,张泽浩,王刚毅,单宇. 城乡一体化进程下农户信息需求影响因素分析[J]. 中国农学通报, 2015, 31(28): 285-290.
[5]	王晓蓉,贾宝红,马雪,信丽媛,王丽娟. 都市农业农户科技信息服务需求的影响因素分析——以天津设施种植业为例[J]. 中国农学通报, 2015, 31(25): 273-276.
[6]	王晓乔廖桂平史晓慧. 农业信息网站服务质量评价指标优化研究[J]. 中国农学通报, 2014, 30(23): 308-315.
[7]	郑春花臧运平. 农业高校图书馆与地方信息网站共建信息服务平台的研究[J]. 中国农学通报, 2013, 29(35): 396-399.
[8]	史晓慧廖桂平陈天恩. 基于需求驱动的茶叶产业互动式信息服务技术[J]. 中国农学通报, 2013, 29(17): 222-226.
[9]	王小宁. 农户信息需求区域差异的实证分析——以陕西为例[J]. 中国农学通报, 2011, 27(29): 157-161.
[10]	王景辉,王丰,郑海新,刘善文,黄惠珍,包巍. “福建省农业科技综合信息管理系统构建研究”[J]. 中国农学通报, 2009, 25(19): 303-308.
[11]	田春可,孟宪学. 浅论4Cs营销理论在农业信息服务中的应用[J]. 中国农学通报, 2007, 23(4): 426-426.
[12]	张博,李思经. 浅谈新农村建设中农业信息服务模式的创新[J]. 中国农学通报, 2007, 23(4): 430-430.
[13]	余秋华,贺立源. 湖北省农业信息体系建设研究[J]. 中国农学通报, 2006, 22(8): 523-523.
[14]	罗红彬,刘光哲. 农业高校图书馆信息服务工作存在的问题及对策刍议[J]. 中国农学通报, 2005, 21(11): 381-381.
[15]	刘恩博, 马富裕郑重李江全高新奇. GSM技术在棉田水分监测中的应用[J]. 中国农学通报, 2004, 20(5): 259-259.