桑花叶萎缩类病毒的检测与序列多态性分析

doi:10.11924/j.issn.1000-6850.2014-1075

中国农学通报 ›› 2014, Vol. 30 ›› Issue (28): 306-309.doi: 10.11924/j.issn.1000-6850.2014-1075

桑花叶萎缩类病毒的检测与序列多态性分析

孔卫青,杨金宏,王蓓,朱晓东,雷晓强

安康学院,安康学院,,安康学院,安康学院

收稿日期:2014-04-15 修回日期:2014-04-15 接受日期:2014-08-06 出版日期:2014-10-15 发布日期:2014-10-15
通讯作者: 孔卫青
基金资助:
陕西省青年科技新星项目“桑树类病毒在非自然宿主中的复制潜能研究”(2013KJXX-96)；安康学院教育教学改革研究项目“基于研究的生物科学类本科毕业设计模式的研究与实践”(Jg06105)；大学生创新创业训练计划项目“桑花叶萎缩类病毒基因组序列与二级结构的多态性研究” (2013sxjy006)

The Detection of Mulberry Mosaic Dwarf Viroid and Analysis of Its Sequence Polymorphism

Received:2014-04-15 Revised:2014-04-15 Accepted:2014-08-06 Online:2014-10-15 Published:2014-10-15

摘要/Abstract

摘要： 为调查桑花叶萎缩类病毒的多态性，通过RT-PCR技术分离了安康地区桑花叶型萎缩类病毒，并对其基因组进行了克隆测序和序列分析。结果获得4 个MMDVd变体，核苷酸长度为356 nt 或357 nt，GC含量均约51%，Mfold网站预测二级结构两端为分枝状结构，其中约有58%的碱基配对。

关键词: 接穗, 接穗

Abstract: To detect the polymorphism of mulberry mosaic dwarf viroid, the sequence of mulberry mosaic dwarf viroid isolates in Ankang were investigated by RT-PCR, cloning and sequence analysis. The results showed that 4 variants of MMDVd were obtained, and the length was from 356 nt to 357 nt. The GC content was about 51% for all. There were about 58% pairs in MMDVd secondary structure predicted in Mfold web, and the two end of it were both branched.

孔卫青,杨金宏,王蓓,朱晓东,雷晓强. 桑花叶萎缩类病毒的检测与序列多态性分析[J]. 中国农学通报, 2014, 30(28): 306-309.

参考文献

[1] Diener T A. Potato spindle tuber "virus". A reblicating low molecular weight RNA[J]. Virology,1971,45(2):411-428.
[2] Semancik J S, Weathers L G. Exocortis disease: evidence for a new species of "infectious" low molecular weigh. RNA in plants[J]. Nature New Biology,1972,237(77):242-244.
[3] 中国科学院上海生物化学研究所病毒组,浙江省农业科学院蚕桑研究所桑病组.桑树萎缩病病原的研究Ⅱ.桑树萎缩病及桑花叶型萎缩病病原体的电子显微镜研究[J].中国科学,1974,3:292-296.
[4] 张月季.桑花叶型萎缩病的研究[J].蚕业科学,1983,9(2):74-79.
[5] 蒯元璋,田波.桑花叶萎缩病病原上的新发现[A].第2次全国桑树保护学讨论论文集[C].1990:40-41.
[6] 卢全友,夏志松.桑花叶型萎缩病病原研究初报[J].蚕业科学,2006, 32(2):249-251.
[7] 费建明.桑花叶萎缩病病原和防治技术研究[D].苏州:苏州大学, 2007.
[8] Wang W B, Fei J M, Wu Y, et al. A new report of a mosaic dwarf viroid-like disease on mulberry trees in China[J]. Polish journal of microbiology,2010,59(1):33-36.
[9] 蒯元璋.桑树病毒与病毒病的研究进展(II)[J].蚕业科学,2011,37 (2):278-284.
[10] Nie X. Analysis of Sequence Polymorphism and Population Structure of Tomato chlorotic dwarf viroid and Potato spindle tuber viroid in Viroid-Infected Tomato Plants[J]. Viruses,2012,4(6):940- 953.
[11] 马先锋,韩健,邓子牛,等.柑橘类病毒的分子鉴定与分型[J].中国农业科学,2008,4(9):2670-2677.
[12] 孔卫青,杨金宏.桑花叶型萎缩病病原的检测与序列分析[J].中国农学通报,2012,28(36):237-240.
[13] Altschul S F, Thomas L M, Alejandro A S, et al. Gapped BLAST and PSI- BLAST: a new generation of protein database search programs[J]. Nucleic Acids Res,1997,25(17):3389-3402.
[14] Zuker M. Mfold web server for nucleic acid folding and hybridization prediction[J]. Nucleic Acids Res,2003,31(13):3406- 3415.
[15] Malfitano M, Di S F, Covelli L, et al. Peach latent mosaic viroid variants inducing peach calico (extreme chlorosis) contain a characteristic insertion that is responsible for this symptomatology [J]. Virology,2003,313(2):492-501.
[16] Jiang D, Gao R, Qin L, et al. Infectious cDNA clones of four viroids in Coleus blumei and molecular characterization of their progeny[J]. Virus Res,2014, 180: 97-101.
[17] Fauquet C M, Mayo M A, Maniloff J, et al. Virus Taxonomy-Eight Report of the International Committee on Taxonomy of Viruses[M]. San Diego: Elsevier Academic Press,2005.
[18] Daros J A, Elena S, Flores R. Viroids: an Ariadne’ s thread into the RNA labyrinth[J]. EMBO Rep,2006,7(6):593-598.

桑花叶萎缩类病毒的检测与序列多态性分析

The Detection of Mulberry Mosaic Dwarf Viroid and Analysis of Its Sequence Polymorphism

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 2

编辑推荐

Metrics

本文评价

关于本刊

作者中心

审者中心

在线期刊

联系我们

[1]	王军周珺刘子凡林位夫. 橡胶树砧穗胶乳养分含量与产量的关系[J]. 中国农学通报, 2014, 30(7): 17-21.
[2]	周珺林位夫蔡明道张日庆. 不同砧木处理方法对3种橡胶树芽接苗生长的影响[J]. 中国农学通报, 2013, 29(16): 29-31.