欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2014, Vol. 30 ›› Issue (36): 120-123.doi: 10.11924/j.issn.1000-6850.2014-1161

所属专题： 生物技术； 水稻

• 生物技术科学 • 上一篇    下一篇

水稻草状矮化病毒侵染下水稻根发育相关基因的表达分析

丁新伦,谢荔岩,潘 贤,吴祖建   

  1. (福建农林大学植物病毒研究所/福建省植物病毒重点实验室/农业部闽台作物有害生物综合治理重点实验室,福州 350028）
  • 收稿日期:2014-04-21 修回日期:2014-11-19 接受日期:2014-11-24 出版日期:2015-03-20 发布日期:2015-03-20
  • 通讯作者: 丁新伦
  • 基金资助:
    福建省教育厅资助科技项目“低温调控水稻条纹病毒与寄主水稻互作的机理研究”(JA12107)。

Transcriptional Analysis of the Root Development Related Genes in Host Rice (Oryza Sativa)
Infected with Rice Grassy Stunt Virus

Ding Xinlun, Xie Liyan, Pan Xian, Wu Zujian   

  1. (Institute of Plant Virology, Fujian Agriculture and Forestry University/Key Laboratory of Plant Virology of Fujian Province/Key Laboratory of Integrated Pest Management for Fujian-Taiwan Crops, Fuzhou 350028)
  • Received:2014-04-21 Revised:2014-11-19 Accepted:2014-11-24 Online:2015-03-20 Published:2015-03-20

摘要： 水稻草状矮化病毒(Rice grassy stunt virus, RGSV)引起的水稻草矮病是热带和亚热带稻区的重要病害，RGSV侵染引起水稻植株根系严重发育不良。为解析RGSV侵染引致水稻根系发育不良的分子机制，以水稻品种‘汕优63’为材料，通过实时荧光定量RT-PCR技术分析水稻经RGSV侵染后根系发育相关基因OsPHR2、QHB、OsRR1、OsEXPB5、Crl-5和OsEXPA17等基因的mRNA表达量变化。结果表明，RGSV侵染引起OsPHR2和Crl-5基因表达下调，下调比率分别为2.18和4.86，而水稻病株QHB、OsRR1、OsEXPB5和OsEXPA17等基因的表达量与健株相比，各基因差异比率分别为1/1.49、1.01、1.39、1/1.37，表明RGSV侵染未引起这些基因的表达量发生变化。RGSV可能通过直接或间接调控根系发育相关基因表达水平而使根系发育异常。

关键词: 凯里市, 凯里市

Abstract: Rice grassy stunt virus (RGSV) in tropical and subtropical areas can cause large losses in rice production and quality. RGSV-infected rice plants show root disorders and the molecular mechanism is still unclear. The transcript levels of root development related genes OsPHR2, QHB, OsRR1, OsEXPB5, Crl-5 and OsEXPA17 in rice plants infected with RGSV versus control rice plants were determined by using real-time quantitative reverse-transcription PCR. The results showed that RGSV infection led to reducing transcript levels of OsPHR2 and Crl-5 genes and the relative expressions reducing rate of OsPHR2 and Crl-5 genes were 2.18 and 4.86, respectively. RGSV infection did not alter the transcript levels of QHB, OsRR1, OsEXPB5 and OsEXPA17 genes whose relative expression levels were 1/1.49, 1.01, 1.39, 1/1.37, respectively. These data suggested that RGSV could cause root disorders by regulating transcription levels of root development related genes directly or indirectly.