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中国农学通报

中国农学通报 ›› 2014, Vol. 30 ›› Issue (30): 1-5.doi: 10.11924/j.issn.1000-6850.2014-1400

所属专题： 生物技术； 水稻

• 农学 农业基础科学 •    下一篇

转3α-HSD 基因水稻对土壤根际微生物影响及 胆固醇降解菌的筛选鉴定

白泽方,潘大仁,霍煜璟,李清,林芳敏   

  1. 福建农林大学,福建农林大学,福建农林大学生命科学学院,福建农林大学作物科学学院,福建农林大学生命科学学院
  • 收稿日期:2014-05-15 修回日期:2014-05-15 接受日期:2014-06-17 出版日期:2014-10-31 发布日期:2014-10-31
  • 通讯作者: 白泽方
  • 基金资助:
    教育部科学技术重点项目“高效降解类固醇的工程菌菌株的改造与筛选”(No.207056)；国家自然科学基金“不同化感型杉木无性系连栽过 程根际酚类物质动态变化及其代谢机理”(31270676)

Effect of 3α-HSD Transgenic Rice on the Microorganism in the Soil and Isolation and Identification of Cholesterol-Degrading Strains in the Soil

  • Received:2014-05-15 Revised:2014-05-15 Accepted:2014-06-17 Online:2014-10-31 Published:2014-10-31

摘要： 通过对转3α-HSD 基因水稻各个时期根际微生物的数量的研究，探究转基因水稻对土壤微生物的影响，并且从种植转基因水稻的土壤中分离纯化出两株具有较强降解胆固醇的菌株。结果表明，转基因水稻的根际微生物在数量上大于常规水稻。通过从土壤中富集分离纯化分离出来的2 个菌株在30℃，180 r/min 的条件下培养4 天后对0.1 mg/mL胆固醇的胆固醇降解率分别达到38.75%和64.64%，通过经形态特征以及16S rDNA序列分析，初步鉴定为肠杆菌科克氏杆菌(Klebsiella pneumoniae)。

关键词: 日较差, 日较差

Abstract: In this paper,3α-HSD transgenic rice rhizosphere microorganism of the soil were cultured and counted to reveal the effects of rhizosphere microbial secretions on the number of microorganisms at different times. And 2 strains that utilized cholesterol as sole carbon and energy sources were isolated from the soil by the enrichment culture method. The results showed that, the quantities of3α-HSD transgenic rice rhizosphere microorganism were greater than that of conventional rice. Two strains were selected for degradation of cholesterol. In the culture conditions of 30℃ and 180 r/min, the degradation rate to cholesterol of 2 strains were 38.75% and 64.64%, with 0.1 mg/mL concentrations within four days. 2 strains were identified to be Klebsiella pneumoniae by morphological characteristics and the similarity analysis of the 16S rDNA sequence.