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中国农学通报 ›› 2005, Vol. 21 ›› Issue (11): 82-82.

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五株食源性致病菌特异性卵黄抗体的研制

霍乃蕊,张 浩,韩克光   

  • 出版日期:2005-11-05 发布日期:2005-11-05

Preparation of Specific Yolk Antibody against 5 Strains of Food Born Pathogenic Bacteria

Huo Nairui, Zhang Hao, Han Keguang   

  • Online:2005-11-05 Published:2005-11-05

摘要: 目的:确定制备用作食品添加剂的引起食品中毒常见的5种致病菌的高免卵黄抗体的技术参数。方法:以食品中常见的由该实验室分离和鉴定的沙门氏菌,金黄色葡萄球菌,溶血性链球菌,副溶血弧菌,变形杆菌五株地方性致病菌株的等量混合菌液用油佐剂乳化后免疫新开产的蛋鸡,收集高免蛋,利用水稀释法制备多克隆卵黄抗体,并用超滤法对其进行浓缩。结果:免疫后高免蛋收集的最佳时间为三免后第2~8周,水稀释法最佳稀释比例为1:6,pH值为5.2,通过超滤IgY浓度提高了6.48倍,IgY的纯度由原来的24.6%提高到42.3%。结论:通过上

Abstract: Objective: determine the technical parameters for the production of high immunity yolk antibody used as food additives against five strains of food born pathogenic bacteria. Methods: Pathogenic Salmonella spp, staphylococcus aureaus, streptococcus haemolit