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中国农学通报 ›› 2017, Vol. 33 ›› Issue (32): 34-38.doi: 10.11924/j.issn.1000-6850.casb16110092

• 林学 园艺 园林 • 上一篇    下一篇

山竹果皮总黄酮富集工艺研究

蔡彬新   

  1. 宁德师范学院生物系
  • 收稿日期:2016-11-18 修回日期:2017-02-20 接受日期:2017-02-25 出版日期:2017-11-27 发布日期:2017-11-27
  • 通讯作者: 蔡彬新
  • 基金资助:
    福建教育厅项目“山竹果皮总黄酮富集工艺及抗氧化特性研究”(JA12342)。

Purification Condition of Flavonoids from Garcinia mangostana Pericarp

蔡彬新   

  • Received:2016-11-18 Revised:2017-02-20 Accepted:2017-02-25 Online:2017-11-27 Published:2017-11-27

摘要: 研究 D101大孔树脂富集山竹总黄酮的工艺参数。通过静态、动态相结合的方法,确定最 佳工艺参数。结果表明D101大孔树脂对总黄酮动态解析附的效果好于静态,动态解析附的最佳 条件为:以流速2mL/min,上样30mL 2mg/mL的提取物,且用55mL 70%乙醇以2mL/min的流 速洗脱,此条件下提取物总黄酮含量由原先的57.8mg/mL增加到174.2mg/mL。

关键词: 稻鸭共作, 稻鸭共作, 生态效应, 种养模式

Abstract: The study aims to clarify the condition for purifying total flavonoids from the pericarp of mangostana. Optimal parameters of purification of total flavonoids were studied through static and dynamic adsorption and desorption behaviors. The results showed that effect of the dynamic adsorption and desorption was better than the static one. The optimum condition of purification was as following: 30 mL 2 mg/mL of mangostana was loaded onto the column at a flow rate of 2 mL/min, and eluted with 60 mL of 70% alcohol at a flow rate of 2 mL/min. Under the condition, the content of total flavonoids was increased from 57.8 mg/mL to 174.2 mg/mL.