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中国农学通报

中国农学通报 ›› 2017, Vol. 33 ›› Issue (23): 68-75.doi: 10.11924/j.issn.1000-6850.casb17010040

• 资源 环境 生态 土壤 气象 • 上一篇    下一篇

黑龙江省盐碱湖泊中微藻的分布及理化因素对优势藻种生物量的影响

何剑波,凌宏志,辛星,平文祥,葛菁萍   

  1. 黑龙江大学生命科学学院/微生物省高校重点实验室,黑龙江大学生命科学学院/微生物省高校重点实验室,黑龙江大学生命科学学院/微生物省高校重点实验室,黑龙江大学生命科学学院/微生物省高校重点实验室,黑龙江大学生命科学学院/微生物省高校重点实验室
  • 收稿日期:2017-01-10 修回日期:2017-07-19 接受日期:2017-02-24 出版日期:2017-08-21 发布日期:2017-08-21
  • 通讯作者: 葛菁萍
  • 基金资助:
    国家自然科学基金面上项目“从2.3 丁二醇代谢角度构建工程微生物群体及其生态学机制研究”(31570492)；国家自然科学基金面上项目 “菌群演替与温水沤麻系统关键酶产生菌代谢组学特征的耦合机制”(31270534)；国家自然科学基金面上项目“副干酪乳杆菌与枯草芽孢杆菌等菌群 种间关系及协同合作策略的研究”(31470537)；黑龙江省高等学校科技创新团队“农业微生物发酵技术”(2012td009)。

Distribution of Microalgae in Saline Lakes of Heilongjiang and Effects of Physical and Chemical Factors on the Biomass of Dominant Algal Strains

  • Received:2017-01-10 Revised:2017-07-19 Accepted:2017-02-24 Online:2017-08-21 Published:2017-08-21

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摘要： 旨在分析黑龙江省盐碱湖泊中微藻的分布及理化因素对优势藻种生物量的影响，本研究以黑龙江省大庆、肇东和扎龙盐碱地区湖泊作为研究对象，从中共分离筛选得到7株藻种，采用Plackett-Burman试验设计筛选出显著影响因子，利用响应面设计对重要影响因素进行优化。结果表明，肇东盐碱湖泊分离筛选出的淡水小球藻（Chorella Sorokiniana）HDA04的生物量和含油量均达到最高。藻种的生物量与环境因子有一定的相关性。在一定条件下，pH和盐度越高，C. sorokiniana HDA04的生物量越高。当葡萄糖21.46 g/L、FeC6H5O7?5H2O 12 mg/L、K2HPO4?3H2O 120 mg/L、MgSO4?7H2O 75 mg/L、pH 8.89、发酵温度32.91℃、转速为120 r/min时，以20%的接种量接种于100 mL的BG11培养基中，C. sorokiniana HDA04生物量达7.855±0.08 g/L，是优化前的1.99倍，生物量水平提高了98.9%。本研究明确了理化因素对C. sorokiniana HDA04生长速率的影响，为微藻的进一步研究奠定基础。

关键词: 龙虾, 龙虾, 水温, 溶解氧, 预报模型

Abstract: To analyze the distribution of microalgae in saline lakes of Heilongjiang Province and the influence of physical and chemical factors on the biomass of dominant algae, the saline area lakes of Daqing, Zhaodong and Zhalong in Heilongjiang Province were taken as the research objects, a total of 7 algae strains were isolated and screened in this study, Plackett- Burman test design was used to screen out the significant influence factors, and response surface design was used to optimize the important factors. The results showed that the biomass and oil content of Chorella sorokiniana HDA04, which was separated in saline lakes of Zhaodong, reached the highest. The algal biomass had certain correlation with environmental factors. Under certain conditions, the higher the pH value and salinity, the higher the biomass of C. sorokiniana HDA04 was. When glucose was 21.46 g/L, FeC6H5O7·5H2O was 12 mg/L, K2HPO4·3H2O was 120 mg/L, MgSO4·7H2O was 75 mg/L, pH value was 8.89, fermentation temperature was 32.91℃, the speed was 120 r/min, inoculated in 100 mL BG11 medium with 20% inculation quantity, C. sorokiniana HDA04 biomass was 7.855±0.08 g/L, which was 1.99 times as much as that before optimization, the biomass level increased by 98.9%. This study clarified the influence of physical and chemical factors on the growth rate of C. sorokiniana HDA04, and laid a foundation for further study on microalgae.

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