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中国农学通报

中国农学通报 ›› 2018, Vol. 34 ›› Issue (6): 30-38.doi: 10.11924/j.issn.1000-6850.casb17070148

所属专题： 棉花

• 农学 农业基础科学 • 上一篇    下一篇

NaCl胁迫对海岛棉幼苗光合和荧光特性的影响

赵海燕,林兴,王建设   

  1. 安阳工学院生物与食品工程学院,安阳工学院生物与食品工程学院,安阳工学院生物与食品工程学院
  • 收稿日期:2017-07-31 修回日期:2018-02-01 接受日期:2017-11-24 出版日期:2018-02-26 发布日期:2018-02-26
  • 通讯作者: 王建设
  • 基金资助:
    棉花生物学国家重点实验室开放课题“毛棉盐胁迫下细胞结构和蛋白质 SWATH 定量分析”(No. CB2015A23)、河南省高等学校重点科研项目“毛棉苗期根部盐胁迫差异蛋白质组学研究”(No. 15A180026)、安阳工学院校科研“海岛棉盐胁迫下生理生化和细胞结构研究”(No. YJJ2015014)、博士启动金项目“毛棉应答盐胁迫转录组测序分析”(No. 400721)和安阳市科技开放项目“异源四倍体棉种耐盐蛋白质资源发掘与鉴定”(No. 57)。

Impacts of NaCl Stress on Characteristics of Photosynthesis and Chlorophyll Fluorescence of Gossypium barbadense at Seedling Stage

  • Received:2017-07-31 Revised:2018-02-01 Accepted:2017-11-24 Online:2018-02-26 Published:2018-02-26

摘要： 本文旨在研究盐胁迫对不同海岛棉品系幼苗生长指标、叶绿素含量、光合及叶绿素荧光参数的影响,为海岛棉耐盐材料的选育、栽培管理提供理论依据。以两份海岛棉—耐盐性强的品系越海9号和耐盐性弱的品系PS-7为材料,采用水培法,从发芽至三叶期,进行150 mM NaCl的持续胁迫,研究盐胁迫下不同海岛棉品系的生长指标、叶绿素含量、光合及荧光参数的变化。结果显示：持续NaCl胁迫降低了越海9号和PS-7的叶面积、根长、茎长、根/茎比值、叶绿素 a、叶绿素 b和叶绿素 a与b总量,提高了叶绿素a/b比值。盐胁迫促进了越海9号的净光合速率(Pn)、气孔导度(Gs)、胞间CO2浓度(Ci)、蒸腾速率(Tr)、水分利用率(WUE)、PSⅡ实际光化学量子产量(Y(Ⅱ))、光化学淬灭系数(qP)、光合电子传递相对速率(rETR)上升,PSII的最大光化学量子产量(Fv/Fm)和非光化学猝灭系数(NPQ)下降。盐胁迫导致PS-7的净光合速率(Pn)、气孔导度(Gs)、蒸腾速率(Tr)、水分利用率(WUE)、PSII的最大光化学量子产量(Fv/Fm)、PSⅡ实际光化学量子产量(Y(Ⅱ))、光化学淬灭系数(qP)和光合电子传递相对速率(rETR)降低,胞间CO2浓度(Ci)和非光化学猝灭系数(NPQ)提高。PS-7的根/茎比值大于越海9号,其余指标均小于越海9号。上述结果提示,持续盐胁迫抑制了海岛棉生长指标及叶绿素等生物量积累,对耐盐性弱的品系抑制程度大于耐盐性强的品系。非气孔限制因素是导致盐胁迫下PS-7净光合速率下降的主要原因。PSⅡ实际原初光能转化效率提高是引起盐胁迫下越海9号净光合速率上升的主要原因。生长指标、叶绿素含量、光合参数、叶绿素荧光参数可作为鉴定棉花耐盐材料的生理学指标。

Abstract: The impacts of salt stress on the growth index, chlorophyll content, photosynthesis and chlorophyll fluorescence parameters of Gossypium barbadense seedlings were studied for providing theoretical evidence on materials breeding, cultivation of salt stress. Two Gossypium barbadense strains, Yuehai 9 (salt tolerance) and PS-7 (salt sensitive) were selected, and germinated in full nutrient solution. 150mM of NaCl was added into solution from germination stage to 3-leaf stage. The response of Gossypium barbadense seedlings to NaCl stress was assessed by measuring the growth index, chlorophyll content, photosynthesis and chlorophyll fluorescence parameters. The results showed that, under continual NaCl stress, the leaf area, root length, stem length, root/stem ratio, chlorophyll a, chlorophyll b, total chlorophyll content were reduced and chlorophyll a/b ratio was enhanced in the Yuehai 9 and PS-7. Salt stress increased net photosynthetic rate (Pn), stomatal conductance (Gs), intercellular CO2 concentration (Ci), transpiration rate (Tr), water use efficiency (WUE), actual photochemical efficiency of PSⅡ (Y(Ⅱ)), photochemical quenching (qP), relative photosynthetic electron transport rate (rETR), and decreased maximal quantum yield of PSⅡ (Fv/Fm), non-photochemical quenching (NPQ) in the Yuehai 9. Salt stress reduced net photosynthetic rate (Pn), stomatal conductance (Gs), transpiration rate (Tr), water use efficiency (WUE), maximal quantum yield of PSⅡ(Fv/Fm), actual photochemical efficiency of PSⅡ(Y(Ⅱ)), photochemical quenching (qP), relative photosynthetic electron transport rate (rETR), and enhanced intercellular CO2 concentration (Ci), non-photochemical quenching (NPQ) in the PS-7.The root/stem ratio in the PS-7 was greater than this in the Yuehai 9, the other indexes in the PS-7 were less than that in the Yuehai 9. Continuing NaCl stress exerted an obviously adverse effect on Gossypium barbadense seedlings growth and biomass accumulation. The strong salt tolerance strain were more tolerant to salt stress than the salt sensitive strain. Under salt stress, the decline of net photosynthetic rate in the PS-7 was restricted by non-stomatal factors, the major reason on the increase of net photosynthetic rate in the Yuehai 9 was the advance of actual photochemical efficiency of PSⅡ. The growth index, chlorophyll content, photosynthesis and chlorophyll fluorescence parameters can be used as reference indexes on the identification of the salt tolerance of the material.