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中国农学通报

中国农学通报 ›› 2004, Vol. 20 ›› Issue (2): 24-24.

所属专题： 园艺

• 目次 • 上一篇    下一篇

结球甘蓝的离体快繁技术研究

柴向华,张秀珊,李军,朱饱卿,曾宝珰   

  • 出版日期:2004-04-05 发布日期:2004-04-05

Study on Rapid Propagation of Brassica oleracea L. var. capitata

Chai Xianghua, Zhang Xiushan, Li Jun, Zhu Baoqing, Zeng Baodang   

  • Online:2004-04-05 Published:2004-04-05

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651

摘要： 以结球甘蓝无菌苗的胚轴和子叶为材料，几乎不生长愈伤组织，分化出不定芽。不定芽在添加6-BA的MS培养基上增殖很快，适宜的生根培养基为1/2MS+IBA0.5mg/L+ NAA0.1mg/L。通过增加培养基中糖和琼脂粉的用量，提高培养时的光照强度等有效控制了玻璃苗的产生。

Abstract: Adventitious buds were inducted almost without the development of calli with the hypocotyl segments and cotyledons of aseptic seedlings of Brassica oleracea L. var. capitata as explants. Cultured on the MS medium supplemented with 6-BA, its propagation rate was highest. The optimum medium for rooting was 1/2MS supplemented with 0.5mg/L IBA and 0.1mg/L NAA. High proportion of sucrose and agar in the medium and intense lightening could control the occurrence of glassy shoot effectively.