农杆菌介导的共转化体系的研究进展

中国农学通报 ›› 2004, Vol. 20 ›› Issue (2): 26-26.

农杆菌介导的共转化体系的研究进展

张红宇汪秀志赵秀云汪旭东吴先军

出版日期:2004-04-05 发布日期:2004-04-05

The advance on Agrobacterium-mediated co-transformation systems

ZHANG Hong-yu WANGXiu-zhi ZHAO Xiu-yun WANG Xu-dong WU Xian-jun

Online:2004-04-05 Published:2004-04-05

摘要/Abstract

摘要： 实现多基因在同一受体植株中的共转化在研究植物多基因控制的代谢途径和获得去选择标记转基因植株中具有巨大的应用前景。本文综述了目前国内外在共转化上的研究进展，尤其对农杆菌介导的共转化系统的新类型、作用机理、影响因素及其应用前景进行了阐述。

张红宇汪秀志赵秀云汪旭东吴先军. 农杆菌介导的共转化体系的研究进展[J]. 中国农学通报, 2004, 20(2): 26-26.

ZHANG Hong-yu WANGXiu-zhi ZHAO Xiu-yun WANG Xu-dong WU Xian-jun. The advance on Agrobacterium-mediated co-transformation systems[J]. Chinese Agricultural Science Bulletin, 2004, 20(2): 26-26.

1. Forreiter C, Kirschner M, Nover L. Stable transformation of an Arabidopsis cell suspension culture with firefly luciferase providing a cellular system for analysis of chaperone activity in vivo. Plant Cell(1997)9:2171-2181
2. De Neve M, De Buck S, Jacobs A, et al. T-DNA integration patterns in co-transformed plant cells suggest that T-DNA repeats originate form co-integration of separate T-DNAs .The Plant J(1997)11:15-29
3. De Buck S, Jacobs A, Van Montagu M, et al, Agrobacterium tumefaciens transformation and co-transformation frequencies of Arabidopsis thaliana root explants and tobacoo protoplasts. MP MI,1998,11:449-457
4. 李雷，刘松梅，胡鸢雷，林忠平。 Cre/lox定位重组系统系统共转化烟草及其精确重组. 科学通报(1999)44:2291-2295
5. Lezek A. Lyznik et al, Stable co-transformation of maize protoplasts with gusA and neo genes. Plant Molecular Biology (1989)13:151-161
6. Hadi Mz et al, Transformation of 12 different plasmids into soybean via particle bombardment.Plant cell reports (1996 MAR) 15(7) :500-505
7. Christou P,Swain W F, Yang N S,et al. Inheritance and expression of foreign genes in transgenic soybean plants. Proc Natl Acad Sci USA(1989)86:7500-7504
8. Ritala A, Aspegren K, Kurten U, et al. Fertile transgenic barley by particle bombardment of immature embryos. Plant Mol Biol(1994)24:317-325
9. 金危危，李宗芸，宁顺斌，凌定厚，李立家，宋运淳。基因枪介导质粒共转化整合模式的FISH研究。科学通报(2001)46(15):1281-1285
10. Ann Depicker et al, Frequencies of simultaneous transformation with different T-DNAs and their relevance to the Agrobacterium/plant cell interaction, Mol Gen Genet(1985)201:477-484
11. Komari T et al, Vectors carrying two separate T-DNAs for co-transformation of higher plants mediated by Agrobacterium and segregation of transformation free from selection markers. Plant J(1996)10:165-174
12. 吕德扬，曹学远，唐顺学，田霞。紫花苜蓿外源基因共转化植株的再生。中国科学C辑(2000)30(4):342-348
13. Sukhapinda K. Spivey R. Shahin E. A. Ri-plasmid as a helper for introducing vector DNA into alfalfa plants. Plant Mol Biol(1987)8:209-216
14. Deley et al, Co-transformation with one Agrobacterium tumfaciens strain containing two binary plasmids as a methods for producing marker-free transgenic plants.Plant cell reports(1998)17:489-496
15. De Block et al, Two T-DNAs co-transformed into Brassica napus by a double Agrobacterium tumefaciens infection are mainly integrated at the same locus.Theor Appl Genet(1991)82:257-263
16. Mcknight TD et al, Segregation into one plant cell from two separate Agrobacterium strains. PLant Mol Biol(1987)8:439-445
17. Poirier Y et al, High-frequency linkage of co-expressing T-DNA transgenic Arabidopsis thaliana transformed by vaccum-infiltration of Agrobacterium tumefaciens. Theoret Appl Genet(2000)100:487-493