德国洋甘菊精油促进皮肤创伤愈合作用研究

doi:10.11924/j.issn.1000-6850.casb18110096

中国农学通报 ›› 2020, Vol. 36 ›› Issue (4): 67-75.doi: 10.11924/j.issn.1000-6850.casb18110096

德国洋甘菊精油促进皮肤创伤愈合作用研究

沈珺莲¹, 张楠¹, 李玉红²(), 姚雷²(), 李双月³

¹ 上海交通大学农业与生物学院,上海 200240
² 上海交通大学设计学院,上海 200240
³ 浙江财经大学东方学院,浙江海宁 314408

收稿日期:2018-11-22 修回日期:2019-03-29 出版日期:2020-02-05 发布日期:2020-02-08
通讯作者: 李玉红,姚雷
作者简介:沈珺莲,女,1994年出生,浙江湖州人,硕士研究生,研究方向为芳香植物精油功效性研究。通信地址：200240 上海市闵行区东川路800号上海交通大学农业与生物学院2-103室,Tel：021-34206606,E-mail：violet316@sjtu.edu.cn。
基金资助:
科技创新专项-重大项目培育（千万级项目培育）基金“芳香植物精油功效性及保健机理研究”

The Effect of Matricaria chamomilla Essential Oil on Skin Wound Healing

Shen Junlian¹, Zhang Nan¹, Li Yuhong²(), Yao Lei²(), Li Shuangyue³

¹ School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240
² School of Design, Shanghai Jiaotong University, Shanghai 200240
³ Zhejiang University of Finance & Economics Dongfang College, Haining Zhejiang 314408;

Received:2018-11-22 Revised:2019-03-29 Online:2020-02-05 Published:2020-02-08
Contact: Li Yuhong,Yao Lei

摘要/Abstract

摘要：

探讨不同浓度的德国洋甘菊(Matricaria chamomilla)精油对成纤维细胞增殖和迁移的影响以及对小鼠皮肤创伤愈合的作用。用CCK-8法检测不同浓度德国洋甘菊精油对成纤维细胞增殖的影响,用划痕法检测其对成纤维细胞迁移的影响,用小鼠切除伤模型检测其对小鼠创伤愈合的影响。结果表明,德国洋甘菊精油可显著促进成纤维细胞增殖和迁移,其促进作用呈剂量依赖性;可显著提高小鼠皮肤创伤愈合速度,增加创面皮肤真皮厚度和成纤维细胞数目。德国洋甘菊精油能促进皮肤创伤愈合,其机制可能与促进成纤维细胞增殖和迁移有关。

关键词: 德国洋甘菊精油, 创伤愈合, 成纤维细胞, 切除伤模型

Abstract:

This study aims to investigate the effects of Matricaria chamomilla essential oil on the proliferation and migration of fibroblasts and the wound healing in mice. The effects of different concentrations of M. chamomilla essential oil on the proliferation of fibroblasts were detected by CCK8 method, while the effects on the migration of fibroblasts were detected by scratch method. Excision wound model was established to evaluate the effects of M. chamomilla essential oil on the wound healing in mice. The results showed that: M. chamomilla essential oil significantly promoted the proliferation and migration of fibroblasts in a dose-dependent manner. Meanwhile, it significantly improved the wound healing rate of mice skin. The number of fibroblasts and the thickness of dermis of the wound skin in essential oil treatment group increased significantly compared with that in the control group. These results indicated that M. chamomilla essential oil could promote skin wound healing, and its mechanism might be related to promoting fibroblast proliferation and migration.

Key words: Matricaria chamomilla essential oil, wound healing, fibroblasts, excision wound model

中图分类号:

R285.5

沈珺莲, 张楠, 李玉红, 姚雷, 李双月. 德国洋甘菊精油促进皮肤创伤愈合作用研究[J]. 中国农学通报, 2020, 36(4): 67-75.

Shen Junlian, Zhang Nan, Li Yuhong, Yao Lei, Li Shuangyue. The Effect of Matricaria chamomilla Essential Oil on Skin Wound Healing[J]. Chinese Agricultural Science Bulletin, 2020, 36(4): 67-75.

图/表 8

参考文献 27

[1]	王恩华 . 病理学[M]. 北京: 高等教育出版社, 2008: 85-95.
[2]	姚雷, 黄健, 池强, 等. 两种甘菊生长习性及精油成分差异性分析[A].广州:中国香料香精学术研讨会[C]. 2004.
[3]	Glowania, Raulin H J, Swoboda C . Effect of chamomile on wound healing-a clinical double-blind study[J]. Zeitschrift Für Hautkrankheiten, 1987,62(17):1267-1271. URL pmid: 3318194
[4]	Jarrahi M . An experimental study of the effects of Matricaria chamomilla extract on cutaneous burn wound healing in albino rats[J]. Nat Prod Res, 2008,22(5):422-427. doi: 10.1080/14786410701591713 URL pmid: 18404562
[5]	Jarrahi M, Vafaei A A, Taherian A A , et al. Evaluation of topical Matricaria chamomilla extract activity on linear incisional wound healing in albino rats[J]. Natural Product Research, 2010,24(8):697-702. doi: 10.1080/14786410701654875 URL pmid: 20432150
[6]	Abdolghaffari, Amir Hossein . Wound healing effect of Rosemary and Chamomile combination in rat[J]. Pharmacologyonline, 2010,3:139-145.
[7]	Rezaie Ali, Mohajeri Daryoush . Mehrdad Comparative assessment of Matricaria chamomilla and zinc oxide on healing of experimental skin wounds on rats[J]. Annals of Biological Research, 2012,3(1):550.
[8]	Tubaro A, Zilli C, Redaelli C , et al. Evaluation of antiinflammatory activity of a chamomile extract after topical application[J]. Planta Med, 1984,50(4):359. doi: 10.1055/s-2007-969734 URL pmid: 17340328
[9]	Stanojevic L P, Marjanovic-Balaban Z R, Kalaba V D , et al. Chemical Composition, Antioxidant and Antimicrobial Activity of Chamomile Flowers Essential Oil (Matricaria chamomilla L.)[J]. Journal of Essential Oil Bearing Plants, 2016,19(8):2017-2028.
[10]	袁艺, 龙子江, 杨俊杰 , 等. 洋甘菊挥发油抗炎作用的研究[J]. 药物生物技术, 2011(1):52-55.
[11]	Sazegar M R, Banakar A, Bahrami N , et al. The antioxidant activity of Chamomile (Matricaria chamomilla L.) extract in sunflower oil[J]. World Applied Sciences Journal, 2010,9(8):973-978.
[12]	Davidson J M . Animal models for wound repair[J]. Archives of Dermatological Research, 1998,290(1):S1-S11. doi: 10.1007/s004030050270 URL pmid: 9522996
[13]	付小兵, 孙同柱, 盛志勇 . 几种用于创伤修复研究的动物模型[J]. 中华实验外科杂志, 1999(5):101-102.
[14]	屈纪富, 程天民, 郝利 . 参与皮肤伤口愈合的细胞及其作用的研究进展[J]. 中国组织工程研究, 2001,5(24):74-75.
[15]	甘桦丽, 解慧琪, 陈晓禾 , 等. NGF对人真皮成纤维细胞增殖、细胞周期、胶原合成及迁移影响的体外研究[J]. 中国修复重建外科杂志, 2009,11:1350-1354.
[16]	Shamsardakani M, Ghannadi A, Rahimzadeh A . Volatile Constituents of Matricaria chamomilla L. from Isfahan, Iran[J]. Iranian Journal of Pharmaceuticalences, 2006,2(1):57-60.
[17]	Aya K L, Stern R . Hyaluronan in wound healing: rediscovering a major player[J]. Wound Repair Regen, 2014,22(5):579-593. doi: 10.1111/wrr.12214 URL pmid: 25039417
[18]	Agyare C, Boakye Y D, Bekoe E O , et al. Review: African medicinal plants with wound healing properties[J]. Journal of Ethnopharmacology, 2016,177:85-100. doi: 10.1016/j.jep.2015.11.008 URL pmid: 26549271
[19]	Pierce G . Platelet-derived growth factor (BB homodimer), transforming growth factor-beta 1, and basic fibroblast growth factor in dermal wound healing. Neovessel and matrix formation and cessation of repair[J]. Am J Pathol, 1992,140(6):1375-1388. URL pmid: 1376557
[20]	Lekic P, McCulloch C A . Periodontal ligament cell population: the central role of fibroblasts in creating a unique tissue[J]. Anat Rec, 1996,245(2):327-341. doi: 10.1002/(SICI)1097-0185(199606)245:2<327::AID-AR15>3.0.CO;2-R URL pmid: 8769671
[21]	张再超, 叶希韵, 刘霞 , 等. 高糖对大鼠皮肤成纤维细胞生长及胶原蛋白合成的影响[J]. 上海交通大学学报:医学版, 2009(3):323-326.
[22]	Darby I A, Hewitson T D . Fibroblast differentiation in wound healing and fibrosis[J]. International review of cytology, 2007,257:143. doi: 10.1016/S0074-7696(07)57004-X URL pmid: 17280897
[23]	Pattanayak S P, Sunita P . Wound healing, anti-microbial and antioxidant potential of Dendrophthoe falcata (L.f) Ettingsh[J]. Journal of Ethnopharmacology, 2008,120(2):241-247. doi: 10.1016/j.jep.2008.08.019 URL pmid: 18790035
[24]	Süntar I, Akkol E K, Keleş H , et al. A novel wound healing ointment: A formulation of Hypericum perforatum oil and sage and oregano essential oils based on traditional Turkish knowledge[J]. Journal of Ethnopharmacology, 2011,134(1):89-96. doi: 10.1016/j.jep.2010.11.061 URL pmid: 21130859
[25]	Hata S, Okamura K, Hatta M , et al. Proteolytic and Non-proteolytic Activation of Keratinocyte-Derived Latent TGF-β1 Induces Fibroblast Differentiation in a Wound-Healing Model Using Rat Skin[J]. Journal of Pharmacological Sciences, 2014,124(2):230-243. doi: 10.1254/jphs.13209fp URL pmid: 24492413
[26]	王益民, 韦福康, 刘敏 . 成纤维细胞与创伤修复的研究进展[J]. 中国修复重建外科杂志, 2000,14(2):126-128.
[27]	Baum C L, Arpey C J . Normal cutaneous wound healing: clinical correlation with cellular and molecular events[J]. Dermatol Surg, 2005,31(6):674-686. doi: 10.1111/j.1524-4725.2005.31612 URL pmid: 15996419

编号	化学成分		相对百分含量/%
编号	中文名	英文名	相对百分含量/%
1	反式β-罗勒烯	trans-β-Ocimene	0.5807
2	β-罗勒烯	β-Ocimene	4.1890
3	蒿酮	ArteMisia ketone	0.2388
4	榄香烯异构体	Elemene isomer	0.8598
5	α环异戊二烯	α-Isocomene	0.3354
6	β-榄香烯	β-Elemene	0.3064
7	β-石竹烯	β-Caryophyllene	0.5165
8	α-律草烯	α-Humulene	0.1634
9	(E)-β-金合欢烯	(E)-β-Farnesene	19.1319
10	脱氢倍半桉叶素	Dehydrosequicineole	0.2322
11	右旋大根香叶烯	Germacrene D	9.8802
12	(Z,E)-α-金合欢烯	(Z,E)-α-farnesene	1.3652
13	双环大根香叶烯	Bicyclogermacrene	7.9543
14	α-金合欢烯	α-Farnesene	13.4396
15	(+)-δ-荜澄茄烯	(+)-δ-Cadinene	0.3832
16	橙花叔醇	Nerolidol	0.7125
17	蓝桉醇	Globulol	0.5952
18	斯巴醇	Spathulenol	1.0679
19	绿花白千层醇	Viridiflorol	0.3488
20	β-桉叶油醇	β-Eudesmol	0.1845
21	澳白檀醇	Lanceol	0.2245
22	τ-荜澄茄醇	τ-Cadinol	0.2903
23	α-氧化红没药醇 B	α-Bisabolol oxide B	4.3743
24	香橙烯氧化物	Aromadendrene oxide	0.304
25	氧化红没药酮 A	Bisabolone oxide A	7.3854
26	母菊薁	Chamazulene	7.9475
27	α-氧化红没药醇 A	α-Bisabolol oxide A	4.4495
28	2-(己-2, 4-二炔-1-叉)-1, 6-二氧螺[4, 4]-3-烯	2-(Hexa-2,4-diyn-1-ylidene)-1,6-dioxaspiro[4.4]non-3-ene	9.9377
29	2-(庚-2, 4-二炔-1-叉)-1, 6-二氧螺[4, 4]-3-烯	2-(Hepta-2,4-diyn-1-ylidene)-1,6-dioxaspiro[4.4]non-3-ene	0.5699
30	二十五烷烃	Pentacosane	0.2634

编号	化学成分		相对百分含量/%
编号	中文名	英文名	相对百分含量/%
1	反式β-罗勒烯	trans-β-Ocimene	0.5807
2	β-罗勒烯	β-Ocimene	4.1890
3	蒿酮	ArteMisia ketone	0.2388
4	榄香烯异构体	Elemene isomer	0.8598
5	α环异戊二烯	α-Isocomene	0.3354
6	β-榄香烯	β-Elemene	0.3064
7	β-石竹烯	β-Caryophyllene	0.5165
8	α-律草烯	α-Humulene	0.1634
9	(E)-β-金合欢烯	(E)-β-Farnesene	19.1319
10	脱氢倍半桉叶素	Dehydrosequicineole	0.2322
11	右旋大根香叶烯	Germacrene D	9.8802
12	(Z,E)-α-金合欢烯	(Z,E)-α-farnesene	1.3652
13	双环大根香叶烯	Bicyclogermacrene	7.9543
14	α-金合欢烯	α-Farnesene	13.4396
15	(+)-δ-荜澄茄烯	(+)-δ-Cadinene	0.3832
16	橙花叔醇	Nerolidol	0.7125
17	蓝桉醇	Globulol	0.5952
18	斯巴醇	Spathulenol	1.0679
19	绿花白千层醇	Viridiflorol	0.3488
20	β-桉叶油醇	β-Eudesmol	0.1845
21	澳白檀醇	Lanceol	0.2245
22	τ-荜澄茄醇	τ-Cadinol	0.2903
23	α-氧化红没药醇 B	α-Bisabolol oxide B	4.3743
24	香橙烯氧化物	Aromadendrene oxide	0.304
25	氧化红没药酮 A	Bisabolone oxide A	7.3854
26	母菊薁	Chamazulene	7.9475
27	α-氧化红没药醇 A	α-Bisabolol oxide A	4.4495
28	2-(己-2, 4-二炔-1-叉)-1, 6-二氧螺[4, 4]-3-烯	2-(Hexa-2,4-diyn-1-ylidene)-1,6-dioxaspiro[4.4]non-3-ene	9.9377
29	2-(庚-2, 4-二炔-1-叉)-1, 6-二氧螺[4, 4]-3-烯	2-(Hepta-2,4-diyn-1-ylidene)-1,6-dioxaspiro[4.4]non-3-ene	0.5699
30	二十五烷烃	Pentacosane	0.2634

天数/d	空白组	对照组	rhEGF组	低浓度德国洋甘菊精油组	中浓度德国洋甘菊精油组	高浓度德国洋甘菊精油组
1	4.14±2.48	5.84±3.84	8.51±3.00^*	7.00±2.60^*	7.58±2.97^*	5.83±3.79
4	26.71±3.66	27.57±4.65	37.39±4.91^*	35.82±5.03^*	39.40±4.16^*	28.90±4.32
7	37.99±2.80	38.92±3.34	51.57±3.07^*	49.33±2.17^*	50.64±4.16^*	45.77±2.54^*
10	86.48±5.84	86.54±6.49	93.99±2.80^*	92.52±3.18^*	95.11±2.79^*	92.79±3.29^*
14	93.31±3.03	93.69±4.78	97.59±0.95^*	96.29±1.67^*	97.04±0.77^*	97.69±0.91^*

天数/d	空白组	对照组	rhEGF组	低浓度德国洋甘菊精油组	中浓度德国洋甘菊精油组	高浓度德国洋甘菊精油组
1	4.14±2.48	5.84±3.84	8.51±3.00^*	7.00±2.60^*	7.58±2.97^*	5.83±3.79
4	26.71±3.66	27.57±4.65	37.39±4.91^*	35.82±5.03^*	39.40±4.16^*	28.90±4.32
7	37.99±2.80	38.92±3.34	51.57±3.07^*	49.33±2.17^*	50.64±4.16^*	45.77±2.54^*
10	86.48±5.84	86.54±6.49	93.99±2.80^*	92.52±3.18^*	95.11±2.79^*	92.79±3.29^*
14	93.31±3.03	93.69±4.78	97.59±0.95^*	96.29±1.67^*	97.04±0.77^*	97.69±0.91^*

项目	空白组	对照组	rhEGF组	低浓度德国洋甘菊精油组	中浓度德国洋甘菊精油组	高浓度德国洋甘菊精油组
成纤维细胞数目	61.42±4.94	67.93±5.67	94.63±9.33^*	80.37±5.51^*	88.45±6.25^*	83.26±6.73^*
炎性细胞数目	45.43±5.78	42.59±3.86	35.39±6.02^*	38.67±4.38	34.42±5.21^*	31.53±3.44^*

德国洋甘菊精油促进皮肤创伤愈合作用研究

The Effect of Matricaria chamomilla Essential Oil on Skin Wound Healing

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 8

参考文献 27

相关文章 7

编辑推荐

Metrics

本文评价

关于本刊

作者中心

审者中心

在线期刊

联系我们

[1]	詹同彤,倪和民,郭勇,王相国. 西门塔尔牛耳皮肤成纤维细胞的体外培养[J]. 中国农学通报, 2019, 35(3): 47-50.
[2]	粟文俊. 脂质体转染陆川猪胎儿成纤维细胞条件的优化[J]. 中国农学通报, 2011, 27(7): 300-303.
[3]	王铁东,逄大欣,欧阳红生. 逆转录病毒载体RNAi技术抑制猪瘟病毒在猪胚胎成纤维细胞的增殖[J]. 中国农学通报, 2009, 25(13): 14-17.
[4]	曹鸿国,李运生,曲秀霞,邓宏魁. 拟胚体对成体细胞参与胚胎嵌合的影响[J]. 中国农学通报, 2008, 24(9): 25-30.
[5]	曹鸿国, ,李运生,曲秀霞. 慢病毒载体介导成纤维细胞高效稳定表达绿色荧光蛋白[J]. 中国农学通报, 2008, 24(7): 6-10.
[6]	效梅,张土保,吴凌锋,安立龙,黎月坚,许英梅,王喜波. 甜菜碱对三黄鸡胚胎原代成纤维细胞生长和增殖的影响[J]. 中国农学通报, 2007, 23(12): 27-27.
[7]	赵晓娥,安志兴,李向臣,高立功,吴月红,山灵,刘凤军,王永胜,张涌. 山羊转t-PA突变体基因的体细胞核移植研究[J]. 中国农学通报, 2005, 21(6): 1-1.