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中国农学通报

中国农学通报 ›› 2021, Vol. 37 ›› Issue (23): 15-22.doi: 10.11924/j.issn.1000-6850.casb2021-0070

所属专题： 生物技术； 水稻

• 生物科学 • 上一篇    下一篇

转基因抗虫水稻‘HD2’不同生长发育阶段cry2A*基因表达量

李荣田1,2(), 于晓晨1,2, 孔梦莹1,2, 刘长华1,2,3()   

  1. 1黑龙江大学生命科学学院,黑龙江省普通高等学校分子生物学重点实验室,哈尔滨 150080
    2黑龙江大学农业微生物技术教育部工程研究中心,哈尔滨 150080
    3黑龙江大学现代农业与生态环境学院,哈尔滨 150080
  • 收稿日期:2021-01-20 修回日期:2021-05-27 出版日期:2021-08-15 发布日期:2021-08-26
  • 通讯作者: 刘长华
  • 作者简介:李荣田,男,1963年出生,吉林德惠人,教授,博士,研究方向:水稻分子育种与分子生物学。通信地址:150080 哈尔滨市南岗区学府路74号 黑龙江大学生命科学学院,Tel:0451-86608243,E-mail: lirongtian07@aliyun.com。
  • 基金资助:
    转基因生物新品种培育国家科技重大专项“粳稻种植区抗虫水稻新品种的培育”(2016ZX08001001-001-007)

Expression Amount of cry2A* Gene at Different Growth and Development Stages of Transgenic Pest-resistant Rice ‘HD2’

Li Rongtian1,2(), Yu Xiaochen1,2, Kong Mengying1,2, Liu Changhua1,2,3()   

  1. 1Key Laboratory of Molecular Biology, College of Heilongjiang Province, School of Life Sciences, Heilongjiang University, Harbin 150080
    2Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang University, Harbin 150080
    3College of Advanced Agriculture and Ecological Environment, Heilongjiang University, Harbin 150080
  • Received:2021-01-20 Revised:2021-05-27 Online:2021-08-15 Published:2021-08-26
  • Contact: Liu Changhua

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摘要：

本研究旨在探究转cry2A*基因抗虫水稻‘HD2-1’等独立系在田间生长发育过程中cry2A*基因表达量及糙米Bt蛋白含量(BPC)。利用qRT-PCR方法和酶联免疫吸附法(ELISA)检测水稻‘HD2-1’等独立系田间不同生长发育阶段组织器官及糙米的cry2A*基因表达量。结果显示,不同水稻独立系cry2A*基因表达量及糙米BPC明显不同,叶片、茎鞘及幼穗cry2A*基因表达量在不同生长发育阶段间往往呈正相关,cry2A*基因mRNA表达量和对应的BPC呈极显著的正相关,糙米BPC和各生长发育阶段各器官cry2A*基因表达量存在正相关趋势。水稻‘HD2’cry2A*基因表达量不同生长发育阶段间及其和糙米BPC间呈正相关。cry2A*基因转录表达越高,BPC越高。

关键词: 转基因抗虫水稻, ‘空育131’, 生长发育阶段, cry2A*基因表达量, Bt蛋白含量(BPC)

Abstract:

The purpose is to investigate the expressing amount of cry2A* gene and brown rice Bt protein content (BPC) in transgenic cry2A* Gene Resistant Rice ‘HD2-1’ and other independent lines during the growth and development stages. qRT-PCR and enzyme-linked immunosorbent assay (ELISA) were used to detect the expression amount of cry2A*gene of organs at different growth and development stages and brown rice BPC. The results showed that there was a significant difference among rice independent lines in expression amount of cry2A* gene and BPC of brown rice. There were often positive correlation of the expression amount of cry2A* gene of leaf blade, sheath-culm and young panicle among different growth and development stages. The mRNA expression of cry2A* gene was positively correlated with the corresponding BPC. And there was a positive correlation trend between BPC of brown rice and expression amount of cry2A* gene of each organ at each stage. In expression amount of cry2A* gene of rice‘HD2’, there were positive relations among different growth and development stages and between the ones and BPC of brown rice. The higher the cry2A* gene transcribed, the higher the BPC was.

Key words: transgenic pest-resistant rice, ‘Kongyu 131’, growth and development stage, expression amount of cry2A* gene, Bt protein content (BPC)

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