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中国农学通报

中国农学通报 ›› 2022, Vol. 38 ›› Issue (33): 28-33.doi: 10.11924/j.issn.1000-6850.casb2022-0023

所属专题： 生物技术； 园艺

• 农学·农业基础科学 • 上一篇    下一篇

甲醇老化处理对甜菜种子发芽的影响

刘镎1(), 胡华兵2, 王荣华2, 刘小越1, 刘朝阳1, 刘晓晗1, 王茂芊1()   

  1. 1黑龙江大学现代农业与生态环境学院，哈尔滨 150080
    2新疆石河子农业科学研究院甜菜所，新疆石河子 832000
  • 收稿日期:2022-01-10 修回日期:2022-05-02 出版日期:2022-11-25 发布日期:2022-11-22
  • 通讯作者: 王茂芊
  • 作者简介:刘镎，男，1998年出生，湖南张家界人，在读硕士，研究方向：种子引发和甜菜分子遗传育种。通信地址：150080 黑龙江省南岗区学府路74号 黑龙江大学现代农业与生态环境学院，Tel：0451-86604650，E-mail：liunaliuna1227@163.com。
  • 基金资助:
    国家糖料产业体系子项目“甜菜优良单胚不育系选育与优势组合创新”(CARS-170113-1);国家糖料产业体系子项目“甜菜种子引发与检测技术研究”(CARS-17011306);国家青年科学基金项目“能源甜菜应答重金属Cd逆境胁迫分子机制的转录组学研究及差异表达基因功能鉴定”(31601229);黑龙江省自然科学基金项目“BvHIPP24基因在能源甜菜重金属镉污染生物修复中的分子机制研究”(LH2019C057)

Methanol Aging Treatment: Effect on Germination of Sugar Beet Seeds

LIU Na1(), HU Huabing2, WANG Ronghua2, LIU Xiaoyue1, LIU Zhaoyang1, LIU Xiaohan1, WANG Maoqian1()   

  1. 1College of Advanced Agriculture and Ecological Environment of Heilongjiang University, Harbin 150080
    2Research Institute of Sugarbeet, Xinjiang Shihezi Academy of Agricultural Sciences, Shihezi, Xinjiang 832000
  • Received:2022-01-10 Revised:2022-05-02 Online:2022-11-25 Published:2022-11-22
  • Contact: WANG Maoqian

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摘要：

为了研究甲醇溶液人工老化处理对甜菜种子发芽指标的影响，探究甜菜种子的耐储性和老化机理，为甜菜种子的人工老化研究提供理论依据。本研究以2020年新收获甜菜种TX2020单粒种为实验材料，设置6个不同时间的老化时间(5、10、15、20、25、30 min)，用甲醇老化法处理种子，通过对甜菜种子进行发芽试验，测定它们的发芽势、发芽率、发芽指数、活力指数、胚根胚轴总长以及种子浸出液电导率。结果显示，种子经过老化处理后各发芽指标均有所下降，与对照相比，种子的发芽势、发芽率、发芽指数、活力指数、胚根胚轴总长分别下降了8.8%~31.41%、13.96%~34.23%、2.66%~22.44%、16.63%~36.44%、7.54%~23.06%，而种子的浸出液电导率则出现上升的情况，较对照上升了34.73%~76.19%。研究结果表明，甲醇溶液能够抑制甜菜种子的萌发，随着时间的不同，甲醇溶液处理甜菜种子在发芽指标上的抑制效果也不同。

关键词: 甜菜种子, 甲醇, 种子老化, 发芽指标

Abstract:

The paper aims to study the effect of artificial aging treatment with methanol solution on the germination index of sugar beet seeds, explore the storage resistance and aging mechanism of sugar beet seeds, and provide a theoretical basis for understanding the artificial aging of sugar beet seeds. In this study, TX2020, a newly harvested single seed, was used as experimental material, and 6 aging time (5, 10, 15, 20, 25 and 30 min) were set up. The seeds were treated with methanol aging method, and the germination test was carried out on the seeds. The germination potential, germination rate, germination index, vigor index, total length of radicle and hypocotyl and conductivity of seed extract were determined. The results showed that germination indexes of seeds decreased after aging treatment. Compared with those of the control, the germination potential, germination rate, germination index, vigor index and total length of radicle and hypocotyl were decreased by 8.8%-31.41%, 13.96%-34.23%, 2.66%-22.44%, 16.63%-36.44% and 7.54%-23.06%, respectively, while the conductivity of seed extract was increased compared with that of the control by 34.73%-76.19%. The results showed that methanol solution could inhibit the germination of sugar beet seeds. With different aging time, the inhibition effect of methanol solution on the germination indexes of sugar beet seeds was also different.

Key words: sugar beet, methanol, seed aging, germination index

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