虎杖对注射无乳链球菌红罗非鱼肝脏酶活性的影响

doi:10.11924/j.issn.1000-6850.casb2022-0163

中国农学通报 ›› 2023, Vol. 39 ›› Issue (18): 151-157.doi: 10.11924/j.issn.1000-6850.casb2022-0163

虎杖对注射无乳链球菌红罗非鱼肝脏酶活性的影响

敬小军¹^,²(), 孔欣茹¹, 华皓坤¹, 刘婷燕¹, 安树伟³, 顾玲玲³

¹ 南京农业大学无锡渔业学院，江苏无锡 214081
² 中国水产科学研究院淡水渔业研究中心，江苏无锡 214081
³ 苏州市吴江区水产技术推广站，江苏苏州 215200

收稿日期:2022-03-09 修回日期:2022-09-22 出版日期:2023-06-25 发布日期:2023-06-25
作者简介:
敬小军，男，1981年出生，四川阆中人，副研究员，博士，研究方向：渔业生态环境保护。通信地址：214081 江苏省无锡市山水西路9号淡水渔业研究中心，Tel：0510-85551590，E-mail：jingxiaojun@ffrc.cn。
基金资助:
现代农业产业技术体系项目“特色淡水鱼产业技术体系岗位科学家”(CARS-46)

Effects of Polygonum cuspidatum on Liver Enzyme Activity of Red Tilapia Injected with Streptococcus agalactiae

JING Xiaojun¹^,²(), KONG Xinru¹, HUA Haokun¹, LIU Tingyan¹, AN Shuwei³, GU Lingling³

¹ Wuxi Fishery College, Nanjing Agricultural University, Wuxi, Jiangsu 214081
² Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, Jiangsu 214081
³ Fisheries Technology Extension Station in Wujiang District of Suzhou City, Suzhou, Jiangsu 215200

Received:2022-03-09 Revised:2022-09-22 Online:2023-06-25 Published:2023-06-25

摘要/Abstract

摘要：

为探究虎杖粗提物和白藜芦醇对注射无乳链球菌红罗非鱼肝脏酶活的影响，在本实验中将红罗非鱼随机分为24组，12组投喂基础日粮，为不加药组，另外12组投喂添加了虎杖粗提物和白藜芦醇的基础日粮，为加药组。在加药组与不加药组中各选取3小组鱼分别注射3个浓度梯度的无乳链球菌菌液，同时设置不注射菌液的对照组，连续观察21 d并记录死亡数量。21 d后检测活鱼肝脏组织中的超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、丙二醛(MDA)、酸性磷酸酶(ACP)、碱性磷酸酶(AKP)和溶菌酶(LZM)的活性。结果显示，当受到低浓度(7×10³CFU/mL)无乳链球菌胁迫时，虎杖粗提物和白藜芦醇可通过增强红罗非鱼鱼体的抗氧化能力来降低链球菌对机体的损害；当受到高浓度(7×10⁵ CFU/mL)无乳链球菌胁迫时，虎杖粗提物和白藜芦醇则通过诱导肝脏中磷酸酶的活性来提高鱼体的抗病力。同时，虎杖粗提物和白藜芦醇在投喂12 d后可使注射无乳链球菌红罗非鱼的死亡率不再增加。该实验表明高、低浓度无乳链球菌胁迫时，虎杖粗提物和白藜芦醇可降低链球菌对鱼体的损害，且控制死亡率。

关键词: 红罗非鱼, 无乳链球菌, 虎杖粗提物, 白藜芦醇

Abstract:

In order to explore the effect of Polygonum cuspidatum crude extract and resveratrol on the liver enzyme activity of red tilapia injected with Streptococcus agalactiae, the red tilapia (Oreochromis mossambicus×O. niloticus) were randomly divided into 24 groups, 12 groups were fed normal diet (no drug addition group), and other 12 groups were fed diet supplemented with P. cuspidatum crude extract and resveratrol (drug addition group) in this study. Three groups of fish were selected from the drug addition group and the no drug addition group to inject S. agalactiae bacterial solution at three concentration gradients respectively. At the same time, the control group was set up without injection of bacterial solution, and all groups were continuously observed for 21 days, and the number of deaths was recorded. The activity of superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA), acid phosphatase (ACP), alkaline phosphatase (AKP) and lysozyme (LZM) in the liver of red tilapia were tested after 21 days. The result showed that P. cuspidatum crude extract and resveratrol reduced the body damage of S. agalactiae by enhancing the antioxidant ability of red tilapia in the low concentration (7×10³ CFU/mL) of S. agalactiae groups. When fish were exposed to high concentration (7×10⁵ CFU/mL) of S. agalactiae, P. cuspidatum crude extract and resveratrol increased the resistance of fish by inducing the activity of phosphatase in the liver. At the same time, the crude extract of P. cuspidatum and resveratrol kept the mortality rate of red tilapia injected with S. agalactiae unchanged after 12 days. This experiment showed that P. cuspidatum crude extract and resveratrol could reduce the damage of S. agalactiae to fish and control the mortality under high and low concentrations of S. agalactiae stress.

Key words: red tilapia, Streptococcus agalactiae, crude extract from Polygonum cuspidatum, resveratrol

敬小军, 孔欣茹, 华皓坤, 刘婷燕, 安树伟, 顾玲玲. 虎杖对注射无乳链球菌红罗非鱼肝脏酶活性的影响[J]. 中国农学通报, 2023, 39(18): 151-157.

JING Xiaojun, KONG Xinru, HUA Haokun, LIU Tingyan, AN Shuwei, GU Lingling. Effects of Polygonum cuspidatum on Liver Enzyme Activity of Red Tilapia Injected with Streptococcus agalactiae[J]. Chinese Agricultural Science Bulletin, 2023, 39(18): 151-157.

图/表 4

图1. 虎杖对注射无乳链球菌红罗非鱼肝脏抗氧化性能的影响数据柱形图无标注字母或标注相同小写字母表示同一实验组（加药组或不加药组）不同处理组组间差异不显著(P>0.05)，标注不同小写字母表示同一实验组不同处理组组间差异显著(P<0.05)。*表示不同实验组在同一处理组水平上差异显著(P<0.05)。下图同。

图2. 虎杖对注射无乳链球菌红罗非鱼肝脏中磷酸酶活性的影响

图3. 虎杖对注射无乳链球菌红罗非鱼肝脏中溶菌酶(LZM)活性的影响

图4. 虎杖对注射无乳链球菌红罗非鱼死亡率的影响

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虎杖对注射无乳链球菌红罗非鱼肝脏酶活性的影响

Effects of Polygonum cuspidatum on Liver Enzyme Activity of Red Tilapia Injected with Streptococcus agalactiae

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