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中国农学通报

中国农学通报 ›› 2023, Vol. 39 ›› Issue (35): 144-149.doi: 10.11924/j.issn.1000-6850.casb2023-0046

• 植物保护 • 上一篇    下一篇

低温对绿盲蝽成虫耐寒性及主要耐寒物质的影响

许敏1,2(), 陈利珍2, 李文静1(), 万鹏1()   

  1. 1 农业农村部华中作物有害生物综合治理重点实验室/农作物病虫草害防控湖北省重点实验室/湖北省农业科学院植保土肥研究所，武汉 430064
    2 华中农业大学植物科技学院，武汉 430070
  • 收稿日期:2023-01-24 修回日期:2023-04-19 出版日期:2023-12-11 发布日期:2023-12-11
  • 通讯作者:
    李文静，女，副研究员，博士，主要从事作物害虫综合防治研究。通信地址：430064 湖北省武汉市洪山区南湖大道18号 湖北省农业科学院植保土肥研究所，Tel：027-88430561，E-mail：；万鹏，男，研究员，博士，主要从事作物病虫害防治工作，E-mail：。
    李文静，女，副研究员，博士，主要从事作物害虫综合防治研究。通信地址：430064 湖北省武汉市洪山区南湖大道18号 湖北省农业科学院植保土肥研究所，Tel：027-88430561，E-mail：；万鹏，男，研究员，博士，主要从事作物病虫害防治工作，E-mail：。
  • 作者简介:

    许敏，女，1994年出生，博士研究生，研究方向：农业昆虫与害虫防治。通信地址：430064 湖北省武汉市洪山区南湖大道18号 湖北省农业科学院植保土肥研究所，E-mail：。

  • 基金资助:
    国家重点研发计划项目“棉花化肥农药减施技术集成研究与示范”(2017YDF0201900); 湖北省农业科技创新项目“农作物重要害虫防控技术研究”(2021-620-000-001-011)

Effect of Low Temperature on Cold Hardiness and the Main Cold Hardiness Substances of Apolygus lucorum Adults

XU Min1,2(), CHEN Lizhen2, LI Wenjing1(), WAN Peng1()   

  1. 1 Key Laboratory of Integrated Pest Management on Crops in Central China, Ministry of Agriculture.P.R.China/Hubei Key Laboratory of Crop Diseases Insect Pests and Weeds Control/Institute of Plant Protection and Soil Fertilizer, Hubei Academy of Agricultural Sciences, Wuhan 430064
    2 College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070
  • Received:2023-01-24 Revised:2023-04-19 Published-:2023-12-11 Online:2023-12-11

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摘要：

旨在明确低温下绿盲蝽的存活能力、过冷却点和结冰点以及体内主要耐寒物质含量的变化，探究低温下绿盲蝽的耐寒能力以及生理响应。设置不同低温处理，测定绿盲蝽成虫的存活率；采用热电偶法，测定不同低温和时间组合下绿盲蝽成虫的过冷却点和结冰点；利用生理生化测定法比较山梨醇、葡萄糖、海藻糖、果糖等生化物质含量的变化。结果表明，低温-5、-10℃处理24 h及以上时间后，绿盲蝽成虫存活率均为0；0、5、15℃处理24 h及以上时间后，绿盲蝽成虫存活率均达96%以上。低温处理能够降低绿盲蝽成虫的过冷却点和结冰点，以5℃处理24 h时下降幅度最大；但处理时间进一步延长至48 h和96 h后，不同低温均不能有效降低过冷却点和结冰点。低温下绿盲蝽成虫体内的耐寒物质含量发生变化：处理24 h后，果糖含量在15℃下增加；处理48 h后，在10~15℃的低温下，果糖、葡萄糖、海藻糖、山梨醇含量增加。研究结果为揭示绿盲蝽的耐寒机理及其预测预报工作提供有益参考。

关键词: 绿盲蝽, 成虫, 低温, 耐寒性, 耐寒物质

Abstract:

This study aims to investigate the physiological response mechanism of A. lucorum adults to low temperature by clarifying the survival, the supercooling point (SCP) and freezing point (FP), as well as the main cold hardiness substances under low temperature. The survival rate of A. lucorum adults was measured by different low temperature treatments. Thermocouple method was used to determine the SCP and FP under different temperature and time. The content of sorbitol, glucose, trehalose and fructose were compared by physiological and biochemical assay. The results showed that after being treated at -5℃ and -10℃ for 24 h or more, the survival rate of A. lucorum adults was 0. After treatment at 0, 5 and 15℃ for 24 h or more, the survival rate was above 96%. After different temperature treatments at 0, 5 and 15℃ for 24 h, the SCP and FP of the adult were decreased. When treated at 5℃ for 24 hours, there was the largest decline. However, when the treatment time extended to 48 h and 96 h, the SCP and FP of the adult were no longer decline. The content of biochemical substances in A. lucorum changed at low temperature. After treatment at 15℃ for 24 h, the content of fructose was increased. After 48 h treatment at a low temperature of 10℃ to 15℃, the contents of fructose, glucose, trehalose and sorbitol were increased. These results provide a reference for revealing the cold tolerance mechanism of A. lucorum and its forecasting.

Key words: Apolygus lucorum, adult, low temperature, cold hardiness, cold hardiness substances