外源褪黑素对低温胁迫下菜豆种子萌发及抗性的影响

doi:10.11924/j.issn.1000-6850.casb2021-1218

中国农学通报 ›› 2022, Vol. 38 ›› Issue (33): 34-38.doi: 10.11924/j.issn.1000-6850.casb2021-1218

所属专题：生物技术

外源褪黑素对低温胁迫下菜豆种子萌发及抗性的影响

杨晓旭¹(), 李梦娣², 刘大军¹, 冯国军¹, 刘畅¹()

¹黑龙江大学现代农业与生态环境学院，哈尔滨 150080
²黑龙江大学生命科学学院，哈尔滨 150080

收稿日期:2021-12-28 修回日期:2022-05-02 出版日期:2022-11-25 发布日期:2022-11-22
通讯作者: 刘畅
作者简介:杨晓旭，男，1988年生人，吉林松原人，助理研究员，博士，研究方向：蔬菜遗传育种与生物技术。通信地址：150006 黑龙江大学现代农业与生态环境学院,农学楼331，Tel：15546226629，E-mail：sunny19880106@126.com。
基金资助:
黑龙江省高校基本科研业务费“菜豆光周期开花调控相关基因的筛选与验证”(2020-KYYWF-1027);“菜豆叶片黄转绿突变体ytg的定位与克隆”(2020-KYYWF-1026)

Exogenous Melatonin Affects the Germination and Resistance Ability of Common Bean Seeds Under Chilling Stress

YANG Xiaoxu¹(), LI Mengdi², LIU Dajun¹, FENG Guojun¹, LIU Chang¹()

¹College of Modern Agriculture and Ecological Environment, Heilongjiang University, Harbin 150080
²College of Life Sciences, Heilongjiang University, Harbin 150080

Received:2021-12-28 Revised:2022-05-02 Online:2022-11-25 Published:2022-11-22
Contact: LIU Chang

摘要/Abstract

摘要：

为了探究褪黑素(Mt)对低温环境下菜豆种子的影响，本研究对菜豆种子进行低温处理(LT)和低温加褪黑素的处理(LT+Mt)，并设置常温处理作为对照(CK)，对同时期的各项生理指标进行测定。结果表明：Mt可以提高低温胁迫下的种子萌发率，但萌发率要低于对照；同时对渗透调节物质、O₂^-和MDA、抗氧化酶活性进行测定，发现低温增加了渗透调节物质的含量，当加入Mt后其含量进一步增加；低温处理后O₂^-和MDA的含量增加，当加入Mt后其含量明显降低，但是要高于对照；低温处理后抗氧化酶活性略低于对照，当加入Mt后其含量显著升高。研究表明外源Mt有效的减轻了低温对种子萌发的损害，从而促进种子萌发过程中抵御低温的能力。

关键词: 菜豆, 褪黑素, 种子, 低温胁迫

Abstract:

In order to explore the effect of melatonin (Mt) on common bean seeds under chilling stress, the seeds of common bean were treated with chilling stress (LT) and chilling stress with melatonin (LT+Mt), and the normal temperature treatment was set as the control (CK). The physiological indexes of seeds of the same period were measured. The results showed that Mt could improve the germination rate of seeds under chilling stress, but the germination rate was lower than that of CK. The content of osmoregulatory substances, O₂^- and MDA and the activities of antioxidant enzymes were measured. It was found that the content of osmoregulatory substances increased under chilling stress, and the content further increased when Mt was added. The content of O₂^- and MDA increased after chilling stress, while the content of MDA decreased significantly after Mt addition, but was higher than that of CK. The activities of antioxidant enzymes after low temperature treatment were slightly lower than those of CK, and the content of antioxidant enzymes was significantly increased when Mt was added. To sum up, exogenous Mt could effectively reduce the damage of chilling stress on seed germination, thus promoting the ability of seeds to resist chilling stress during germination.

Key words: common bean, melatonin, seed, chilling stress

中图分类号:

S643.1

杨晓旭, 李梦娣, 刘大军, 冯国军, 刘畅. 外源褪黑素对低温胁迫下菜豆种子萌发及抗性的影响[J]. 中国农学通报, 2022, 38(33): 34-38.

YANG Xiaoxu, LI Mengdi, LIU Dajun, FENG Guojun, LIU Chang. Exogenous Melatonin Affects the Germination and Resistance Ability of Common Bean Seeds Under Chilling Stress[J]. Chinese Agricultural Science Bulletin, 2022, 38(33): 34-38.

图/表 4

图1. 低温胁迫下外源Mt对菜豆种子发芽的影响

图2. 低温胁迫下外源Mt对菜豆种子脯氨酸和可溶性糖含量的影响

图3. 低温胁迫下外源Mt对菜豆种子O2-和MDA含量的影响

图4. 低温胁迫下外源Mt对菜豆种子SOD和POD活性的影响

参考文献 27

[1]	KIDD F, WEST C. The influence of temperature on the soaking of seeds[J]. New phytologist, 1919, 18(1-2):35-39. doi: 10.1111/j.1469-8137.1919.tb07284.x URL
[2]	HUANG Z, ZOU Z, HE C, et al. Physiological and photosynthetic responses of melon (Cucumis melo L.) seedlings to three Glomus species under water deficit[J]. Plant soil, 2011, 339(1-2):391-399. doi: 10.1007/s11104-010-0591-z URL
[3]	郝晶, 张立军, 谢甫绨. 低温对大豆不同耐冷性中萌发期保护酶活性的影响[J]. 大豆科学, 2007, 26(2):171-175.
[4]	郭志强, 侯立白, 李霞, 等. 抗低温助长剂对低温胁迫下玉米生长发育及生理生化变化的影响[J]. 玉米科学, 2008, 16(3):82-85.
[5]	刘日林, 章玉婷, 潘凌洁, 等. 低温对不同抗性菜豆品种生理机制的影响[J]. 浙江农业学报, 2015, 27(2):189-193.
[6]	HARDELAND R, FUHRBERG B. Ubiquitous melatonin-Presence and effects in unicells,plants and animals[J]. Trends in comparative biochemistry physiology, 1996, 2:25-45.
[7]	REITER R J, TAN D X. Melatonin: an antioxidant in edible plants[J]. Annals of the new york academy of sciences, 2002, 957(1):341-344. doi: 10.1111/j.1749-6632.2002.tb02938.x URL
[8]	CHEN G, HUO Y, TAN D X, et al. Melatonin in chinese medicinal herbs[J]. Life sciences, 2003, 73(01):19-26. doi: 10.1016/S0024-3205(03)00252-2 URL
[9]	HARDELAND R, POEGGELER B. Non vertebrate melatonin[J]. Journal of pineal research, 2003, 34:233-241. doi: 10.1034/j.1600-079X.2003.00040.x URL
[10]	HARDELAND R, PANDI-PERUMAL S R. Melatonin, a potent agent in antioxidative defense: actions as a natural food constituent,gastrointestinal factor, drug and prodrug[J]. Nutrition metabolism, 2005, 2(1):22. doi: 10.1186/1743-7075-2-22 URL
[11]	POSMYK M M, BAABUSTA M, WIECZOREK M, et al. Melatonin applied to cucumber (Cucumis sativus L.) seeds improves germination during chilling stress[J]. Journal of pineal research, 2009, 46(2):14-223.
[12]	包宇. 外源褪黑素对低温胁迫下番茄幼苗生理指标的影响[D]. 重庆: 西南大学, 2014:34-35.
[13]	SAIRAM R K, SRIVASTAVA G C. Changes in antioxidant activity in sub-cellular fractions of tolerant and susceptible wheat genotypes in response to long term salt stress[J]. Plant sci, 2002, 162(6):897-904. doi: 10.1016/S0168-9452(02)00037-7 URL
[14]	DHINDSA R S, MATOWE W. Drought tolerance in rwo mosses: correlated with enzymatic defence against lipid peroxidation[J]. Journal of experimental botany, 1981, 32(126):79-91. doi: 10.1093/jxb/32.1.79 URL
[15]	张志良, 瞿伟菁. 植物生理学实验指导. 第3版[M]. 北京: 高等教育出版社, 2003:25-28.
[16]	李合生. 植物生理生化实验原理和技术[M]. 北京: 高等教育出版社, 2000:28-29.
[17]	GIANNOPOLITES C N, RIES S K. Superoxide dismutase occurrence in higher plants[J]. Plant physiology, 1977, 59:309-314. doi: 10.1104/pp.59.2.309 URL
[18]	PATTERSON B D, MACRAE E A, FERGUSON I B. Estimation of hydrogen-peroxide in plant-extracts using titanium(iv)[J]. Analytical biochemistry, 1984, 139:487-492. doi: 10.1016/0003-2697(84)90039-3 URL
[19]	刘建新, 胡浩斌, 王鑫. 外源一氧化氮供体对镉胁迫下黑麦草幼苗活性氧代谢、光合作用和叶黄素循环的影响[J]. 环境科学学报, 2009, 29(3):626-633.
[20]	ZHANG R P, MA J, CAI G Z, et al. Effects of low temperature stress during flowering stage on flowering and seed setting of rice in Panxi Region, Sichuan Province[J]. Acta agronomica sinica, 2012, 38(9):1734-1742. doi: 10.3724/SP.J.1006.2012.01734 URL
[21]	郑光华, 梁峥, 林坚, 等. 种子吸胀冷害和渗透调控的研究[J]. 中国科学院院刊, 2001, 16(3):182-187.
[22]	李佳荫, 冯国军, 刘大军, 等. 低温胁迫对菜豆种子吸胀期间生理特性和发芽能力的影响[J]. 中国农学通报, 2020, 36(1):24-29.
[23]	胡俊杰, 张古文, 胡齐赞, 等. 低温胁迫对菜用大豆生长、叶片活性氧及多胺代谢的影响[J]. 浙江农业学报, 2011, 23(6):1113-1118.
[24]	郭云平. NaCl胁迫对西瓜幼苗的影响及腐植酸的缓解效应[D]. 泰安: 山东农业大学, 2016:25-26.
[25]	李欠敏, 杨琳, 段巧红, 等. 外源褪黑素对低温胁迫下大白菜幼苗生长及生理特性的影响[J]. 山东农业大学学报:自然科学版, 2021, 52(2):182-186.
[26]	史中飞, 梁娟红, 张小花, 等. 外源褪黑素对低温胁迫下油菜幼苗抗寒性的影响[J]. 干旱地区农业研究, 2019, 37(4):163-170.
[27]	田雨菁, 胡雅琦. 外源褪黑素对非生物胁迫下植物生长发育的影响[J]. 生物化工, 2020, 6(4):163-164,170.

外源褪黑素对低温胁迫下菜豆种子萌发及抗性的影响

Exogenous Melatonin Affects the Germination and Resistance Ability of Common Bean Seeds Under Chilling Stress

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 4

参考文献 27

相关文章 15

编辑推荐

Metrics

本文评价

关于本刊

作者中心

审者中心

在线期刊

联系我们

[1]	王思之, 关文灵, 郝晓函, 宋杰. 平铺白珠种子萌发特性研究[J]. 中国农学通报, 2023, 39(1): 77-84.
[2]	高萌, 张冬野, 冯国军, 杨晓旭, 刘畅, 闫志山, 刘大军. 外源硒对⁶⁰Co-γ辐射下菜豆幼苗生长和生理的影响[J]. 中国农学通报, 2022, 38(7): 35-40.
[3]	许昕阳, 张跃建, 沈佳, 寿伟松. 厚皮甜瓜‘翠雪5号’种子破休眠方法研究[J]. 中国农学通报, 2022, 38(7): 41-44.
[4]	李雪枫, 王坚, 叶晓园, 张秀婷, 王丽学. 苦瓜植株水浸提液对水稻种子萌发和秧苗生长的影响[J]. 中国农学通报, 2022, 38(6): 1-7.
[5]	闫蕴韬, 何兮, 张海清, 贺记外. 水稻种子耐贮性研究进展[J]. 中国农学通报, 2022, 38(5): 1-8.
[6]	刘镎, 胡华兵, 王荣华, 刘小越, 刘朝阳, 刘晓晗, 王茂芊. 甲醇老化处理对甜菜种子发芽的影响[J]. 中国农学通报, 2022, 38(33): 28-33.
[7]	吕薇, 李胜男, 冯国军, 杨晓旭, 刘畅, 闫志山, 刘大军. 外源褪黑素降低黄瓜体内霜霉威残留的生理生化分析[J]. 中国农学通报, 2022, 38(28): 107-113.
[8]	杨然, 兴旺, 刘大丽, 吴则东, 王茂芊. 不同浓度褪黑素对甜菜种子的引发影响[J]. 中国农学通报, 2022, 38(27): 19-25.
[9]	谢丰璞, 徐蓉蓉, 高静, 关思静, 葛甜甜, 张亚丽, 王楠, 颜永刚, 李铂, 黄文静. 褪黑素对扁茎黄芪种子萌发和幼苗生长的影响[J]. 中国农学通报, 2022, 38(25): 60-71.
[10]	丛俊超, 胡华兵, 王荣华, 刘大丽, 吴则东, 王茂芊. 不同浓度柠檬酸对甜菜种子的引发影响[J]. 中国农学通报, 2022, 38(20): 13-19.
[11]	银珊珊, 周国彦, 顾博文, 武春成, 闫立英, 谢洋. 褪黑素引发对干旱胁迫下黄瓜幼苗生理特性的影响[J]. 中国农学通报, 2022, 38(19): 30-36.
[12]	秦尧, 刘畅, 刘大军, 冯国军, 杨晓旭. 外源水杨酸对高温胁迫下菜豆种子萌发的影响[J]. 中国农学通报, 2022, 38(18): 79-85.
[13]	陈思琪, 孙敬爽, 麻文俊, 王军辉, 赵曦阳, 胡瑞阳. 植物低温胁迫调控机制研究进展[J]. 中国农学通报, 2022, 38(17): 51-61.
[14]	孙音, 郝军, 房义福, 张谦, 姜楠南. ⁶⁰Co-γ辐射对兜兰组培苗的诱变效应[J]. 中国农学通报, 2022, 38(15): 45-52.
[15]	刘小越, 兴旺, 刘大丽, 吴则东, 王茂芊. 苹果酸对甜菜种子的活力影响研究[J]. 中国农学通报, 2022, 38(12): 33-40.