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中国农学通报

中国农学通报 ›› 2022, Vol. 38 ›› Issue (27): 19-25.doi: 10.11924/j.issn.1000-6850.casb2021-1193

所属专题： 生物技术； 园艺

• 农学·农业基础科学 • 上一篇    下一篇

不同浓度褪黑素对甜菜种子的引发影响

杨然(), 兴旺, 刘大丽, 吴则东, 王茂芊()   

  1. 黑龙江大学现代农业与生态环境学院,哈尔滨 150080
  • 收稿日期:2021-11-20 修回日期:2022-03-28 出版日期:2022-10-05 发布日期:2022-09-21
  • 通讯作者: 王茂芊
  • 作者简介:杨然,女,1998年出生,江苏连云港人,硕士研究生,研究方向：甜菜种子引发和遗传育种。通信地址：150080 黑龙江省南岗区学府路74号 黑龙江大学现代农业与生态环境学院,Tel：0451-86604650,E-mail： 785125876@qq.com。
  • 基金资助:
    国家糖料产业体系项目“甜菜种质资源鉴定与新种质创制”(CARS-170102);国家糖料产业体系子项目“甜菜种子引发与检测技术研究”(CARS-17011306);国家青年科学基金项目“能源甜菜应答重金属Cd逆境胁迫分子机制的转录组学研究及差异表达基因功能鉴定”(31601229);黑龙江省自然科学基金项目“BvHIPP24基因在能源甜菜重金属镉污染生物修复中的分子机制研究”(LH2019C057);黑龙江省省属高等学校基本科研业务费基础研究项目“不同根冠比甜菜品种的生理特性及转录组-蛋白组联合分析研究”(2021-KYYWF-0022)

Initiation Effects of Different Concentrations of Melatonin on Sugar Beet Seeds

YANG Ran(), XING Wang, LIU Dali, WU Zedong, WANG Maoqian()   

  1. College of Advanced Agriculture and Ecological Environment of Heilongjiang University, Harbin 150080
  • Received:2021-11-20 Revised:2022-03-28 Online:2022-10-05 Published:2022-09-21
  • Contact: WANG Maoqian

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摘要：

研究不同浓度的褪黑素对同一类型甜菜种子的引发作用,为甜菜种子包衣材料的筛选奠定了理论基础。本研究以STm1619甜菜种子为试验材料,设置了3种浓度和3个时间梯度的9个处理,通过对甜菜种子浸泡回干发芽培养,测定它们的发芽势、发芽率、发芽指数以及活力指数。结果显示,50 μmol/L浓度处理的STm1619甜菜种子在8、12、24 h的发芽势、发芽率、发芽指数以及活力指数均明显高于对照种子;100 μmol/L浓度处理的STm1619甜菜种子在不同时间的发芽势、发芽率、发芽指数和活力指数高于对照组;200 μmol/L浓度处理的STm1619甜菜种子在8、12、24 h的发芽势、发芽率、发芽指数和活力指数也都较高于对照组。不同浓度褪黑素对STm1619甜菜种子的萌发随着时间不同,效果也各不相同。处理时间过长或过短都会对引发效果产生一定的影响。

关键词: 甜菜, 种子, 褪黑素, 种子引发, 种子包衣剂

Abstract:

The study aims to explore the initiation effect of different concentrations of melatonin on the same type of beet seeds, and lay a theoretical foundation for the screening of beet seed coating materials. In this study, STm1619 beet seeds were used as experimental materials, and 9 treatments with 3 concentrations and 3 time gradients were set up. By soaking beet seeds back to dry germination culture, the germination potential, germination rate, germination index and vigor index were measured. The results showed that the germination potential, germination rate, germination index and vigor index of STm1619 beet seeds treated with 50 μmol/L for 8, 12 and 24 h were significantly higher than those of the control. The germination potential, germination rate, germination index and vigor index of STm1619 beet seeds treated with 100 μmol/L for different time were higher than those of the control. The germination potential, germination rate, germination index and vigor index of beet seeds treated with 200 μmol/L for 8, 12 and 24 h were also higher than those of the control. The effects of different concentrations of melatonin on seed germination of STm1619 sugar beet varied with time. Too long or too short processing time will have a certain impact on the initiation effect.

Key words: sugar beet, seed, melatonin, seed initiation, seed coating agent

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