基质胶提取制备与应用的研究进展

doi:10.11924/j.issn.1000-6850.casb2023-0327

中国农学通报 ›› 2024, Vol. 40 ›› Issue (8): 6-11.doi: 10.11924/j.issn.1000-6850.casb2023-0327

基质胶提取制备与应用的研究进展

张涵漪(), 王天一, 路惠添, 张涛()

北京农学院动物科学技术学院，北京 102206

收稿日期:2023-04-28 修回日期:2023-07-18 出版日期:2024-03-15 发布日期:2024-03-10
通讯作者:
张涛，男，1980年出生，河南夏邑人，副教授，博士，主要从事中兽医药对微血管内皮细胞功能调控的研究。Tel：010-80793027，E-mail：zhangtao@bua.edu.cn。
作者简介:
张涵漪，女，2002年出生，北京人，本科，研究方向：小鼠肿瘤模型的建立。通信地址：102206 北京市昌平区史各庄街道北农路7号北京农学院科技综合楼714，E-mail：1206769860@qq.com。
基金资助:
国家自然科学基金面上项目“黄芪多糖对PRRSV感染PAMs致肺MVECs功能障碍的调控作用及其机制研究”(32273050)

Research Progress on the Extraction, Preparation and Uses of Matrigel

ZHANG Hanyi(), WANG Tianyi, LU Huitian, ZHANG Tao()

Animal Science and Technology College, Beijing University of Agriculture, Beijing 102206

Received:2023-04-28 Revised:2023-07-18 Published-:2024-03-15 Online:2024-03-10

摘要/Abstract

摘要：

基底膜是细胞外基质的特化结构，存在于大多数的组织之中，不仅为上皮细胞、内皮细胞、神经细胞、肌肉细胞等提供支持结构，而且发挥着维持组织结构和功能的重要作用。基质胶是从富含胞外基质蛋白的EHS小鼠肿瘤中提取的可溶性基底膜制备物，它含有几乎所有的基底膜成分，能良好模拟基底膜细胞外基质的生物学特性，为生命科学研究中多种实验模型的构建提供了关键生物材料，尤其是类器官培养的重要载体。综述了基质胶的生物学特性、提取制备方法及主要用途，讨论了基质胶潜在应用领域与价值，分析了国内外基质胶生产的现状与存在的问题，并对基质胶产品的未来发展趋势进行了展望，以期对中国基质胶产品的研发及应用提供参考。

关键词: EHS肿瘤, 基质胶, 基底膜, 细胞外基质, 类器官

Abstract:

The basement membrane is a specialized structure of extracellular matrix, which exists in most tissues. It not only provides supporting structure for various cell types such as epithelial cells, endothelial cells, nerve cells, muscle cells, but also plays an important role in maintaining the tissue structure and function. Matrigel is the soluble basement membrane preparation extracted from the mouse Engelbreth-Holm-Swarm (EHS) tumors rich in extracellular matrix protein. It contains almost all components of the basement membrane and can mimic its biological properties, which becomes the crucial biological material for many experimental models in life science research, especially the important media of organoids. In this review, the biological characteristics of matrigel, its extraction and preparation technology, and its main uses are summarized, and its potential application fields and values are discussed. Additionally, the current status of its production both at home and abroad and the existing problems are analyzed, and the future development trend of matrigel production is prospected. This study aims to provide reference for the development and application of matrigel in China.

Key words: Engelbreth-Holm-Swarm tumor, matrigel, basement membrane, extracellular matrix, organoid

张涵漪, 王天一, 路惠添, 张涛. 基质胶提取制备与应用的研究进展[J]. 中国农学通报, 2024, 40(8): 6-11.

ZHANG Hanyi, WANG Tianyi, LU Huitian, ZHANG Tao. Research Progress on the Extraction, Preparation and Uses of Matrigel[J]. Chinese Agricultural Science Bulletin, 2024, 40(8): 6-11.

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基质胶提取制备与应用的研究进展

Research Progress on the Extraction, Preparation and Uses of Matrigel

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