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中国农学通报 ›› 2024, Vol. 40 ›› Issue (8): 12-20.doi: 10.11924/j.issn.1000-6850.casb2023-0267

• 林学·园艺·园林 • 上一篇    下一篇

皂荚枝条对低温胁迫的生理响应及抗寒性评价

陈思1(), 韩丽君1, 郝向春1, 周帅1,2(), 张峻尘2   

  1. 1 山西省林业和草原科学研究院,太原 030012
    2 山西农业大学林学院,山西太谷 030800
  • 收稿日期:2023-04-11 修回日期:2023-06-13 出版日期:2024-03-15 发布日期:2024-03-10
  • 通讯作者:
    周帅,男,1987年出生,山西太原人,工程师,博士研究生,研究方向:林木良种选育与森林培育。通信地址:030012 山西省太原市新建南路105号 山西省林业和草原科学研究院,Tel:0351-7224376,E-mail:
  • 作者简介:

    陈思,女,1989年出生,山西交城人,工程师,硕士研究生,研究方向:林木良种选育与森林培育。通信地址:030012 山西省太原市新建南路105号 山西省林业和草原科学研究院,Tel:0351-7224376,E-mail:

  • 基金资助:
    山西省科技成果转化引导专项“皂荚良种培育及野皂荚改造技术成果推广”(202204021301069); 山西省林业重点研发计划专项“皂荚无性系抗寒性研究及评价”(2022LYCX03); 中央财政林业科技推广示范项目“皂荚良种‘帅荚1号’繁育与栽培技术示范”(晋〔2022〕TG13号)

Physiological Response and Cold Resistance Evaluation of the Shoots of Gleditsia sinensis under Low Temperature Stress

CHEN Si1(), HAN Lijun1, HAO Xiangchun1, ZHOU Shuai1,2(), ZHANG Junchen2   

  1. 1 Shanxi Academy of Forestry and Grassland Sciences, Taiyuan 030012
    2 College of Forestry, Shanxi Agricultural University, Taigu, Shanxi 030800
  • Received:2023-04-11 Revised:2023-06-13 Published-:2024-03-15 Online:2024-03-10

摘要:

通过对皂荚不同无性系枝条抗寒能力差异进行分析,为皂荚抗寒品种的选育及栽培提供理论依据。以11个皂荚无性系1年生枝条为试验材料,测定分析其在不同低温条件下(5℃、0℃、-5℃、-10℃、-15℃、-20℃、-25℃ )的相对电导率,并以此为基础进行主成分分析及聚类分析,测定不同抗寒能力的皂荚无性系枝条组织解剖结构、反射光谱及MDA、SOD含量,并探讨无性系间抗寒性差异的原因。皂荚枝条相对电导率对低温的响应随着温度的降低呈现上升趋势,但部分无性系呈“升-降-升”的趋势;抗寒性较强的无性系特点是枝条的木质部在枝横截面积中所占比例较大,皮层和韧皮部所占比例较小,NDVI、ARI2、SIPI、CRI2参数较高,MDA含量低而SOD酶活性高。11个供试皂荚无性系枝条抗寒性由高到低排序为:‘羊角皂’>‘LKHD5’>‘北山底’>‘帅荚三号’>‘牛家峪’>‘302’>‘河林一号’>‘涧头村’>‘西贾二号’>‘LKHD4’>‘LKSD’。

关键词: 皂荚, 无性系, 低温, 相对电导率, 反射光谱, 保护酶

Abstract:

This study aims to assess the cold resistance of the shoots of G. sinensis by analyzing the difference of cold resistance among its different clones, providing theoretical basis for the cultivation and breeding of cold resistant varieties of G. sinensis. One-year-old shoots of 11 clones of G. sinensis were selected as experimental materials, the relative electrical conductivity (REC) of them under different low temperature conditions (5 ℃, 0 ℃, -5 ℃, -10 ℃, -15 ℃, -20 ℃, -25 ℃) was measured and analyzed, respectively. Principal component analysis and cluster analysis were used based on the results of REC. The anatomical structure of shoots, reflection spectrum, contents of malondialdehyde (MDA) and activities of super oxide dismutase (SOD) were studied under low temperature stress to analyze the difference of cold resistance among the clones. The results showed that there were some differences in the variations of REC in the shoots of all clones under the low temperature stress. There were some characteristics in clones with a stronger cold resistance, including a larger ratio of xylem, a smaller ratio of cortex and phloem in the cross-sectional area of branches, and higher values of NDVI, ARI2, SIPI and CRI2 parameters. At the same time, these clones presented the characteristics of low MDA contents and high SOD activities. The cold resistance of the 11 shoots of G. sinensis was in order of ‘Yangjiaozao’>‘LKHD5’>‘Beishandi’>‘Shuaijia 3 hao’>‘Niujiayu’>‘302’>‘Helin 1 hao’>‘Jiantoucun’>‘Xijia 2 hao’>‘LKHD4’>‘LKSD’.

Key words: Gleditsia sinensis, clones, low temperature, relative electrical conductivity, reflection spectrum, protective enzyme