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中国农学通报

中国农学通报 ›› 2024, Vol. 40 ›› Issue (15): 117-123.doi: 10.11924/j.issn.1000-6850.casb2023-0163

• 生物科学 • 上一篇    下一篇

枯草芽孢杆菌CH-1降解羽毛产物对Cr(Ⅵ)的还原作用

李茹桥(), 刘颖, 陈清旺, 吴嘉沛, 陈金军()   

  1. 湖南农业大学生物科学技术学院，长沙 410128
  • 收稿日期:2023-02-28 修回日期:2023-06-15 出版日期:2024-05-23 发布日期:2024-05-23
  • 通讯作者:
    陈金军，女，1975年出生，湖南益阳人，教授，研究生，博士，研究方向：遗传学。通信地址：410000 湖南省长沙市芙蓉区农大路一号湖南农业大学，Tel：0731-84673601，E-mail：。
  • 作者简介:

    李茹桥，女，2002年出生，湖南长沙人，本科，研究方向：生物科学专业。通信地址：410000 湖南省长沙市芙蓉区农大路一号湖南农业大学，Tel：19507499221，E-mail：。

  • 基金资助:
    国家自然科学基金“三种蜘蛛毒素多肽作用于α2δ-1-NMDAR的镇痛功能与机制研究”(32170529); 湖南省自然科学基金“介导神经病理疼痛的重要受体复合物α2δ-1-NMDAR的分子细胞机制”(2023JJ30286)

Reduction of Cr(Ⅵ) by Feather Degradation Products of Bacillus subtilis CH-1

LI Ruqiao(), LIU Ying, CHEN Qingwang, WU Jiapei, CHEN Jinjun()   

  1. College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128
  • Received:2023-02-28 Revised:2023-06-15 Published:2024-05-23 Online:2024-05-23

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摘要：

本研究旨在探索一种对环境友好的方法以减少重金属六价铬Cr(Ⅵ)的毒性。采用枯草芽孢杆菌(Bacillus subtilis) CH-1发酵羽毛，制备出富含半胱氨酸的羽毛发酵液(Feather Fermentation Broth, FFB)。实验结果显示FFB能够有效地将有毒的六价铬还原为相对无害的三价铬。通过对FFB的β-角蛋白酶酶活性、半胱氨酸浓度以及对六价铬Cr(VI)的还原率的测定。我们发现FFB中β-角蛋白酶酶活性在21 h左右达到最大值，而半胱氨酸含量在36 h达到最高值，Cr(VI)的还原效果最佳时间也是36 h。值得注意的是，即使经过高压高温处理，FFB对Cr(VI)的还原力并未受到影响，表明还原过程不依赖于生物酶的作用。进一步实验，将FFB上清原液浓缩5倍后处理重铬酸钾溶液，发现浓缩液的还原效果是原始上清液效果的1.5倍。研究表明枯草芽孢杆菌CH-1能较好地降解羽毛废弃物，水解产物中含大量半胱氨酸，对Cr(VI) 具有显著的还原能力。综上所述，本研究提供了一种使用枯草芽孢杆菌CH-1无公害处理羽毛废弃物的新策略，并为其在循环农业生态系统中的应用提供了理论依据和精确计算方法。这一发现对于实现农业废弃物资源化利用，以及环境中有毒六价铬的安全处理具有重要意义。

关键词: Cr(Ⅵ)还原, 羽毛水解产物, 枯草芽孢杆菌CH-1, 角蛋白酶活性, 半胱氨酸, 环境治理

Abstract:

In order to develop a strategy for reducing hexavalent chromium Cr(VI) in heavy metals, feather fermentation with Bacillus subtilis CH-1 was conducted to produce feather fermentation liquid (FFB). The high toxicity of Cr(VI) was reduced to low toxicity trivalent chromium using the abundant cysteine in FFB. The activity of β-keratinase, concentration of cysteine and reduction rate of Cr(VI) in FFB were determined. In FFB, the maximum β-keratinase activity of FFB was reached at about 21st hour, the highest cysteine concentration was reached at 36th hour, and the optimal time for Cr(VI) reduction was also 36 hours. The reduction effect of FFB on Cr(VI) was not affected by high pressure and high temperature treatment, indicating that no biological enzymes were involved in the reduction process. Furthermore, when the FFB supernatant stock solution was concentrated 5 times and then treated with potassium dichromate solution, it was found that the reduction effect of the concentrated solution was 1.5 times that of the original supernatant. These results show that Bacillus subtilis CH-1 can effectively degrade feather waste, and the hydrolysate contains a large amount of cysteine, which has a strong reduction effect on Cr(VI). The present study provides a candidate strain and accurate theoretical basis for the pollution-free treatment and utilization of feather waste, which has a good application prospect and value in circular agro-ecosystem.

Key words: Cr(Ⅵ) reduction, feather hydrolysate, Bacillus subtilis CH-1, keratinase activity, L-cysteine, environmental governance