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中国农学通报

中国农学通报 ›› 2026, Vol. 42 ›› Issue (17): 34-41.doi: 10.11924/j.issn.1000-6850.casb2025-0644

• 生物科学 • 上一篇    下一篇

转cry2A*基因抗虫早粳稻目的基因整合及其表达

田崇兵(), 赵凤民, 孙海正, 孙淑红, 薛菁芳, 张希瑞, 冯延江, 马文东, 胡月婷()   

  1. 黑龙江省农业科学院水稻研究所， 黑龙江佳木斯 154026
  • 收稿日期:2025-08-01 修回日期:2025-12-11 出版日期:2026-09-15 发布日期:2026-09-09
  • 通讯作者:
    胡月婷，女，1987年出生，黑龙江大庆人，副研究员，硕士，主要从事水稻遗传育种及新品种选育工作。通信地址：154026 黑龙江省佳木斯市东风区建国镇 黑龙江省农业科学院水稻研究所，E-mail：。
  • 作者简介:

    田崇兵，男，1987年出生，黑龙江佳木斯人，助理研究员，硕士，主要从事水稻遗传育种及新品种选育工作。通信地址：154026 黑龙江省佳木斯市东风区建国镇 黑龙江省农业科学院水稻研究所，E-mail：。

  • 基金资助:
    黑龙江省省属科研院所科研业务费项目“基于BSA-seq的寒地粳稻高产机理遗传解析”(CZKYF2026-1-B121)

Integration and Expression of Target Gene in cry2A*-Transgenic Early Japonica Rice with Insect Resistance

TIAN Chongbing(), ZHAO Fengmin, SUN Haizheng, SUN Shuhong, XUE Jingfang, ZHANG Xirui, FENG Yanjiang, MA Wendong, HU Yueting()   

  1. Rice Research Institute, Heilongjiang Academy of Agricultural Sciences, Jiamusi, Heilongjiang 154026
  • Received:2025-08-01 Revised:2025-12-11 Published:2026-09-15 Online:2026-09-09

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摘要：

本研究旨在探究转cry2A*基因抗虫早粳稻中目的基因的表达特性。本研究选取了T9代转cry2A*基因早粳稻‘松粳9号(cry2A*)’为材料，采用qPCR技术测定了其在分蘖期、抽穗期各器官的cry2A*基因转录水平，通过ELISA技术定量分析了分蘖期、抽穗期、灌浆期各器官及完熟期糙米中的Bt蛋白表达量。结果显示，cry2A*基因的转录水平在不同转化事件的相同组织间以及同一转化事件的不同组织间均表现出显著差异，抽穗期的转录水平高于分蘖期，在组织间的分布规律为：叶片>幼穗>茎鞘；Bt蛋白表达量在时间上表现为：叶片与茎鞘的蛋白含量呈现灌浆期>抽穗期>分蘖期的趋势，在幼穗中，抽穗期的蛋白含量高于灌浆期，完熟期糙米中的Bt蛋白浓度最低，空间上表现为：分蘖期叶片>茎鞘，抽穗期叶片>幼穗>茎鞘，灌浆期叶片>茎鞘>幼穗，所有这些时空表达量均高于完熟期糙米的表达量。综上，cry2A*基因在早粳稻中的转录水平与其Bt蛋白表达量之间存在显著正相关关系，并且存在明显的时间和空间特异性，整体而言，其表达量在营养器官中随生长发育的推进而升高，而在生殖器官中随生育期的推进而降低。

关键词: 转cry2A*基因早粳稻, ‘松粳9号(cry2A*)’, qPCR技术, ELISA技术, Bt蛋白表达量, 时空表达

Abstract:

To investigate the expression characteristics of the target gene in transgenic cry2A* insect resistant early japonica rice, the study employed the T9 generation of transgenic cry2A* early japonica rice line ‘Songjing 9 (cry2A*)’ as experimental material. The transcription levels of the cry2A* gene in various organs at the tillering and heading stages were detected by quantitative real-time PCR (qPCR), and the expression levels of Bt protein in various organs at the tillering, heading and filling stages as well as in brown rice at the full ripening stage were determined by enzyme-linked immunosorbent assay (ELISA).The results showed that the transcription levels of the cry2A* gene varied among the same tissues of different transformation events and among different tissues of the same transformation event. The transcription levels at the heading stage were higher than those at the tillering stage, and the tissue-specific expression pattern was in the order of leaves > young panicles > culm-sheaths. In terms of temporal expression, the Bt protein levels showed the pattern of filling stage > heading stage > tillering stage in leaves and culm-sheaths, and heading stage > filling stage in young panicles; the Bt protein concentration in brown rice was the lowest at the full ripening stage. In terms of spatial expression, the pattern was leaves> culm-sheaths at the tillering stage, leaves>young panicles>culm-sheaths at the heading stage, and leaves > culm-sheaths > young panicles at the filling stage, with the Bt protein levels in all the above tissues consistently higher than those in brown rice at the full ripening stage. In conclusion, the transcription levels of the cry2A* gene in early japonica rice were significantly positively correlated with the Bt protein expression levels, with distinct temporal and spatial variations. Collectively, the expression increased with the progression of growth and development in vegetative organs, while it decreased with the advance of growth stages in reproductive organs.

Key words: cry2A*-transgenic early japonica rice, ‘Songjing 9 (cry2A*)’, qPCR technology, ELISA technology, Bt protein expression levels, temporal and spatial expression

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